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. Author manuscript; available in PMC: 2011 Nov 9.
Published in final edited form as: Biochemistry. 2010 Nov 9;49(44):9438–9448. doi: 10.1021/bi100532a

Figure 2. PSC inhibits mononucleosomes with one long linker or two linkers better than mononucleosomes with one short linker.

Figure 2

A-C) Representative REAs on a series of mononucleosome templates. The three templates are all 247-bp long. D) PSC does not inhibit restriction enzyme digestion. Naked DNA at 1nM was incubated with PSC under identical conditions as in the remodeling assays either with or without the restriction enzyme HhaI. E) Summary of REAs on the various mononucleosome templates. Note that even though remodeling of templates with linkers can be inhibited by PSC, higher concentrations are required than for 2N templates (compare with Figure 1C). Error bars are SEM.