Abstract
The capacity of interferon-gamma to regulate the generation and release of leukotriene B4 (LTB4) from human alveolar macrophages of normal nonsmoking individuals was evaluated. When alveolar macrophages were incubated for 60 min with heat aggregated IgG (HAIgG), they generated and released 5.7 +/- 1.7 ng of LT B4 per 10(6) cells compared to 1.9 +/- 0.4 ng from cells incubated with buffer alone, P = 0.02. When alveolar macrophages were preincubated with interferon-gamma for 24 h before activation for 60 min with heat-aggregated IgG, the soluble IgG aggregates became a significantly more effective stimulus for LTB4 release, 17.0 +/- 3.9 ng/10(6) cells, P = 0.001, compared to cells incubated in the absence of interferon-gamma and challenged with HAIgG. Interferon-gamma did not alter the response to A23187. This effect of interferon-gamma was both time and dose dependent; it also was specific since neither interferon-alpha nor interferon-beta had a regulatory effect on the release of LTB4 from cells in response to challenge with HAIgG. Preincubation of the alveolar macrophages with interferon-gamma augmented the density of IgG1 receptors by 81.5 +/- 17.3%; neither interferon-alpha nor interferon-beta effected this parameter. Furthermore, monomeric IgG1 blocked HAIgG induced LTB4 release from alveolar macrophages primed with interferon-gamma. Therefore, at least one of the mechanisms by which interferon-gamma primes alveolar macrophages for the production and release of LTB4 in response to stimulation by aggregates of IgG is that of increasing the number of receptors for this stimulus.
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Selected References
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- Aderem A. A., Cohen D. S., Wright S. D., Cohn Z. A. Bacterial lipopolysaccharides prime macrophages for enhanced release of arachidonic acid metabolites. J Exp Med. 1986 Jul 1;164(1):165–179. doi: 10.1084/jem.164.1.165. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Atluru D., Goodwin J. S. Control of polyclonal immunoglobulin production from human lymphocytes by leukotrienes; leukotriene B4 induces an OKT8(+), radiosensitive suppressor cell from resting, human OKT8(-) T cells. J Clin Invest. 1984 Oct;74(4):1444–1450. doi: 10.1172/JCI111556. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Benveniste J. Paf-acether (platelet-activating factor). Adv Prostaglandin Thromboxane Leukot Res. 1985;13:11–18. [PubMed] [Google Scholar]
- Bigby T. D., Holtzman M. J. Enhanced 5-lipoxygenase activity in lung macrophages compared to monocytes from normal subjects. J Immunol. 1987 Mar 1;138(5):1546–1550. [PubMed] [Google Scholar]
- Boraschi D., Censini S., Bartalini M., Tagliabue A. Regulation of arachidonic acid metabolism in macrophages by immune and nonimmune interferons. J Immunol. 1985 Jul;135(1):502–505. [PubMed] [Google Scholar]
- Bray M. A. The pharmacology and pathophysiology of leukotriene B4. Br Med Bull. 1983 Jul;39(3):249–254. doi: 10.1093/oxfordjournals.bmb.a071828. [DOI] [PubMed] [Google Scholar]
- Bremm K. D., König W., Spur B., Crea A., Galanos C. Generation of slow-reacting substance (leukotrienes) by endotoxin and lipid A from human polymorphonuclear granulocytes. Immunology. 1984 Oct;53(2):299–305. [PMC free article] [PubMed] [Google Scholar]
- Celada A., Gray P. W., Rinderknecht E., Schreiber R. D. Evidence for a gamma-interferon receptor that regulates macrophage tumoricidal activity. J Exp Med. 1984 Jul 1;160(1):55–74. doi: 10.1084/jem.160.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cunningham A. L., Merigan T. C. Leu-3+ T cells produce gamma-interferon in patients with recurrent herpes labialis. J Immunol. 1984 Jan;132(1):197–202. [PubMed] [Google Scholar]
- Czarnetzki B. M., Rosenbach T. Chemotaxis of human neutrophils and eosinophils towards leukotriene B4 and its 20-w-oxidation products in vitro. Prostaglandins. 1986 May;31(5):851–858. doi: 10.1016/0090-6980(86)90018-3. [DOI] [PubMed] [Google Scholar]
- Fels A. O., Pawlowski N. A., Cramer E. B., King T. K., Cohn Z. A., Scott W. A. Human alveolar macrophages produce leukotriene B4. Proc Natl Acad Sci U S A. 1982 Dec;79(24):7866–7870. doi: 10.1073/pnas.79.24.7866. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ferreri N. R., Howland W. C., Spiegelberg H. L. Release of leukotrienes C4 and B4 and prostaglandin E2 from human monocytes stimulated with aggregated IgG, IgA, and IgE. J Immunol. 1986 Jun 1;136(11):4188–4193. [PubMed] [Google Scholar]
- Ford-Hutchinson A. W., Bray M. A., Doig M. V., Shipley M. E., Smith M. J. Leukotriene B, a potent chemokinetic and aggregating substance released from polymorphonuclear leukocytes. Nature. 1980 Jul 17;286(5770):264–265. doi: 10.1038/286264a0. [DOI] [PubMed] [Google Scholar]
- Forti R. L., Mitchell W. M., Hubbard W. C., Workman R. J., Forbes J. T. Pleiotropic activities of human interferons are mediated by multiple response pathways. Proc Natl Acad Sci U S A. 1984 Jan;81(1):170–174. doi: 10.1073/pnas.81.1.170. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Friedman R. M., Vogel S. N. Interferons with special emphasis on the immune system. Adv Immunol. 1983;34:97–140. doi: 10.1016/s0065-2776(08)60378-8. [DOI] [PubMed] [Google Scholar]
- Goetzl E. J. Mediators of immediate hypersensitivity derived from arachidonic acid. N Engl J Med. 1980 Oct 2;303(14):822–825. doi: 10.1056/NEJM198010023031421. [DOI] [PubMed] [Google Scholar]
- Gonzalez-Molina A., Spiegelberg H. L. A subpopulation of normal human peripheral B lymphcytes that bind IgE. J Clin Invest. 1977 Apr;59(4):616–624. doi: 10.1172/JCI108679. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goodwin J. S., Atluru D., Sierakowski S., Lianos E. A. Mechanism of action of glucocorticosteroids. Inhibition of T cell proliferation and interleukin 2 production by hydrocortisone is reversed by leukotriene B4. J Clin Invest. 1986 Apr;77(4):1244–1250. doi: 10.1172/JCI112427. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Guyre P. M., Morganelli P. M., Miller R. Recombinant immune interferon increases immunoglobulin G Fc receptors on cultured human mononuclear phagocytes. J Clin Invest. 1983 Jul;72(1):393–397. doi: 10.1172/JCI110980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ham E. A., Soderman D. D., Zanetti M. E., Dougherty H. W., McCauley E., Kuehl F. A., Jr Inhibition by prostaglandins of leukotriene B4 release from activated neutrophils. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4349–4353. doi: 10.1073/pnas.80.14.4349. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hamilton T. A., Rigsbee J. E., Scott W. A., Adams D. O. gamma-Interferon enhances the secretion of arachidonic acid metabolites from murine peritoneal macrophages stimulated with phorbol diesters. J Immunol. 1985 Apr;134(4):2631–2636. [PubMed] [Google Scholar]
- Hayes E. C., Lombardo D. L., Girard Y., Maycock A. L., Rokach J., Rosenthal A. S., Young R. N., Egan R. W., Zweerink H. J. Measuring leukotrienes of slow reacting substance of anaphylaxis: development of a specific radioimmunoassay. J Immunol. 1983 Jul;131(1):429–433. [PubMed] [Google Scholar]
- Henson P. M., Johnson H. B., Spiegelberg H. L. The release of granule enzymes from human neutrophils stimulated by aggregated immunoglobulins of different classes and subclasses. J Immunol. 1972 Dec;109(6):1182–1192. [PubMed] [Google Scholar]
- Hocking W. G., Golde D. W. The pulmonary-alveolar macrophage (second of two parts). N Engl J Med. 1979 Sep 20;301(12):639–645. doi: 10.1056/NEJM197909203011205. [DOI] [PubMed] [Google Scholar]
- Kurlander R. J., Haney A. F., Gartrell J. Human peritoneal macrophages possess two populations of IgG Fc receptors. Cell Immunol. 1984 Jul;86(2):479–490. doi: 10.1016/0008-8749(84)90403-9. [DOI] [PubMed] [Google Scholar]
- Laviolette M., Coulombe R., Picard S., Braquet P., Borgeat P. Decreased leukotriene B4 synthesis in smokers' alveolar macrophages in vitro. J Clin Invest. 1986 Jan;77(1):54–60. doi: 10.1172/JCI112301. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Le J., Prensky W., Yip Y. K., Chang Z., Hoffman T., Stevenson H. C., Balazs I., Sadlik J. R., Vilcek J. Activation of human monocyte cytotoxicity by natural and recombinant immune interferon. J Immunol. 1983 Dec;131(6):2821–2826. [PubMed] [Google Scholar]
- Lewis R. A., Austen K. F. The biologically active leukotrienes. Biosynthesis, metabolism, receptors, functions, and pharmacology. J Clin Invest. 1984 Apr;73(4):889–897. doi: 10.1172/JCI111312. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lüderitz T., Schade U., Rietschel E. T. Formation and metabolism of leukotriene C4 in macrophages exposed to bacterial lipopolysaccharide. Eur J Biochem. 1986 Mar 3;155(2):377–382. doi: 10.1111/j.1432-1033.1986.tb09501.x. [DOI] [PubMed] [Google Scholar]
- Martin T. R., Altman L. C., Albert R. K., Henderson W. R. Leukotriene B4 production by the human alveolar macrophage: a potential mechanism for amplifying inflammation in the lung. Am Rev Respir Dis. 1984 Jan;129(1):106–111. doi: 10.1164/arrd.1984.129.1.106. [DOI] [PubMed] [Google Scholar]
- Martinez-Maza O., Andersson U., Andersson J., Britton S., De Ley M. Spontaneous production of interferon-gamma in adult and newborn humans. J Immunol. 1984 Jan;132(1):251–255. [PubMed] [Google Scholar]
- Metz S. A., Hall M. E., Harper T. W., Murphy R. C. Rapid extraction of leukotrienes from biologic fluids and quantitation by high-performance liquid chromatography. J Chromatogr. 1982 Dec 10;233:193–201. doi: 10.1016/s0378-4347(00)81746-6. [DOI] [PubMed] [Google Scholar]
- Michel F. B., Godard P., Damon M., Chavis C., Crastes De Paulet A. Chemical mediators of anaphylaxis released by alveolar macrophages in bronchial asthma. Eur J Respir Dis Suppl. 1986;146:189–194. [PubMed] [Google Scholar]
- Migliorisi G., Folkes E., Pawlowski N., Cramer E. B. In vitro studies of human monocyte migration across endothelium in response to leukotriene B4 and f-Met-Leu-Phe. Am J Pathol. 1987 Apr;127(1):157–167. [PMC free article] [PubMed] [Google Scholar]
- Murray H. W., Rubin B. Y., Rothermel C. D. Killing of intracellular Leishmania donovani by lymphokine-stimulated human mononuclear phagocytes. Evidence that interferon-gamma is the activating lymphokine. J Clin Invest. 1983 Oct;72(4):1506–1510. doi: 10.1172/JCI111107. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Naegel G. P., Young K. R., Jr, Reynolds H. Y. Receptors for human IgG subclasses on human alveolar macrophages. Am Rev Respir Dis. 1984 Mar;129(3):413–418. doi: 10.1164/arrd.1984.129.3.413. [DOI] [PubMed] [Google Scholar]
- Nagy L., Lee T. H., Goetzl E. J., Pickett W. C., Kay A. B. Complement receptor enhancement and chemotaxis of human neutrophils and eosinophils by leukotrienes and other lipoxygenase products. Clin Exp Immunol. 1982 Mar;47(3):541–547. [PMC free article] [PubMed] [Google Scholar]
- Náray-Fejes-Tóth A., Guyre P. M. Recombinant human immune interferon induces increased IgE receptor expression on the human monocyte cell line U-937. J Immunol. 1984 Oct;133(4):1914–1919. [PubMed] [Google Scholar]
- Orchansky P., Rubinstein M., Fischer D. G. The interferon-gamma receptor in human monocytes is different from the one in nonhematopoietic cells. J Immunol. 1986 Jan;136(1):169–173. [PubMed] [Google Scholar]
- Payan D. G., Goetzl E. J. Recognition of leukotriene B4 by a unique subset of human T-lymphocytes. J Allergy Clin Immunol. 1984 Sep;74(3 Pt 2):403–406. doi: 10.1016/0091-6749(84)90138-6. [DOI] [PubMed] [Google Scholar]
- Rankin J. A., Hitchcock M., Merrill W. W., Huang S. S., Brashler J. R., Bach M. K., Askenase P. W. IgE immune complexes induce immediate and prolonged release of leukotriene C4 (LTC4) from rat alveolar macrophages. J Immunol. 1984 Apr;132(4):1993–1999. [PubMed] [Google Scholar]
- Rankin J. A. IgE immune complexes induce leukotriene B4 release from rat alveolar macrophages. Ann Inst Pasteur Immunol. 1986 May-Jun;137C(3):364–367. doi: 10.1016/s0771-050x(86)80056-0. [DOI] [PubMed] [Google Scholar]
- Rankin J. A., Naegel G. P., Schrader C. E., Matthay R. A., Reynolds H. Y. Air-space immunoglobulin production and levels in bronchoalveolar lavage fluid of normal subjects and patients with sarcoidosis. Am Rev Respir Dis. 1983 Apr;127(4):442–448. doi: 10.1164/arrd.1983.127.4.442. [DOI] [PubMed] [Google Scholar]
- Reynolds H. Y., Chrétien J. Respiratory tract fluids: analysis of content and contemporary use in understanding lung diseases. Dis Mon. 1984 Feb;30(5):1–103. doi: 10.1016/0011-5029(84)90008-7. [DOI] [PubMed] [Google Scholar]
- Robinson B. W., McLemore T. L., Crystal R. G. Gamma interferon is spontaneously released by alveolar macrophages and lung T lymphocytes in patients with pulmonary sarcoidosis. J Clin Invest. 1985 May;75(5):1488–1495. doi: 10.1172/JCI111852. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rokach J., Hayes E. C., Girard Y., Lombardo D. L., Maycock A. L., Rosenthal A. S., Young R. N., Zamboni R., Zweerink H. J. The development of sensitive and specific radioimmunoassays for leukotrienes. Prostaglandins Leukot Med. 1984 Jan;13(1):21–25. doi: 10.1016/0262-1746(84)90098-2. [DOI] [PubMed] [Google Scholar]
- Rola-Pleszczynski M., Gagnon L., Sirois P. Leukotriene B4 augments human natural cytotoxic cell activity. Biochem Biophys Res Commun. 1983 Jun 15;113(2):531–537. doi: 10.1016/0006-291x(83)91758-8. [DOI] [PubMed] [Google Scholar]
- Showell H. J., Naccache P. H., Borgeat P., Picard S., Vallerand P., Becker E. L., Sha'afi R. I. Characterization of the secretory activity of leukotriene B4 toward rabbit neutrophils. J Immunol. 1982 Feb;128(2):811–816. [PubMed] [Google Scholar]
- Sigal C. E., Valone F. H., Holtzman M. J., Goetzl E. J. Preferential human eosinophil chemotactic activity of the platelet-activating factor (PAF) 1-0-hexadecyl-2-acetyl-sn-glyceryl-3-phosphocholine (AGEPC). J Clin Immunol. 1987 Mar;7(2):179–184. doi: 10.1007/BF00916012. [DOI] [PubMed] [Google Scholar]
- Smith M. J., Ford-Hutchinson A. W., Bray M. A. Leukotriene B: a potential mediator of inflammation. J Pharm Pharmacol. 1980 Jul;32(7):517–518. doi: 10.1111/j.2042-7158.1980.tb12985.x. [DOI] [PubMed] [Google Scholar]
- Sobrero A. F., Moir R. D., Bertino J. R., Handschumacher R. E. Defective facilitated diffusion of nucleosides, a primary mechanism of resistance to 5-fluoro-2'-deoxyuridine in the HCT-8 human carcinoma line. Cancer Res. 1985 Jul;45(7):3155–3160. [PubMed] [Google Scholar]
- Wardlaw A. J., Moqbel R., Cromwell O., Kay A. B. Platelet-activating factor. A potent chemotactic and chemokinetic factor for human eosinophils. J Clin Invest. 1986 Dec;78(6):1701–1706. doi: 10.1172/JCI112765. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Williams J. D., Czop J. K., Austen K. F. Release of leukotrienes by human monocytes on stimulation of their phagocytic receptor for particulate activators. J Immunol. 1984 Jun;132(6):3034–3040. [PubMed] [Google Scholar]
- Williams J. D., Lee T. H., Lewis R. A., Austen F. Intracellular retention of the 5-lipoxygenase pathway product, leukotriene B4, by human neutrophils activated with unopsonized zymosan. J Immunol. 1985 Apr;134(4):2624–2630. [PubMed] [Google Scholar]
- Zlotnik A., Shimonkevitz R. P., Gefter M. L., Kappler J., Marrack P. Characterization of the gamma-interferon-mediated induction of antigen-presenting ability in P388D1 cells. J Immunol. 1983 Dec;131(6):2814–2820. [PubMed] [Google Scholar]