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. 1989 Jun;83(6):1925–1929. doi: 10.1172/JCI114099

17-beta-estradiol increases mitogenic activity of medium from cultured preadipocytes of massively obese persons.

S C Cooper 1, D A Roncari 1
PMCID: PMC303913  PMID: 2723065

Abstract

Having reported that omental preadipocytes from massively obese persons release into the culture medium proteins mitogenic for preadipocytes, this study aimed to determine whether estrogens contribute to the production of these factors. Sub-cultured omental preadipocytes from 13 massively obese women were grown in the presence or absence of 17-beta-estradiol, and during the last 24 h the conditioned medium was prepared in the absence of serum. Media from cells of 8 of 13 subjects contained significantly higher mitogenic activity when grown in the presence of 17-beta-estradiol. 17-Alpha-estradiol was not effective. The bioassay system involved rat perirenal preadipocytes, since these have been well characterized. Partial purification by gel filtration chromatography indicated that the estrogen-dependent factors had Mr greater than 250,000 and approximately 30,000. Thus, estrogens might contribute to the development of massive obesity in genetically susceptible subjects by promoting the production of paracrine/autocrine principles by adipose cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aitken S. C., Lippman M. E., Kasid A., Schoenberg D. R. Relationship between the expression of estrogen-regulated genes and estrogen-stimulated proliferation of MCF-7 mammary tumor cells. Cancer Res. 1985 Jun;45(6):2608–2615. [PubMed] [Google Scholar]
  2. Dickson R. B., Huff K. K., Spencer E. M., Lippman M. E. Induction of epidermal growth factor-related polypeptides by 17 beta-estradiol in MCF-7 human breast cancer cells. Endocrinology. 1986 Jan;118(1):138–142. doi: 10.1210/endo-118-1-138. [DOI] [PubMed] [Google Scholar]
  3. Dickson R. B., Kasid A., Huff K. K., Bates S. E., Knabbe C., Bronzert D., Gelmann E. P., Lippman M. E. Activation of growth factor secretion in tumorigenic states of breast cancer induced by 17 beta-estradiol or v-Ha-ras oncogene. Proc Natl Acad Sci U S A. 1987 Feb;84(3):837–841. doi: 10.1073/pnas.84.3.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dickson R. B., Lippman M. E. Estrogenic regulation of growth and polypeptide growth factor secretion in human breast carcinoma. Endocr Rev. 1987 Feb;8(1):29–43. doi: 10.1210/edrv-8-1-29. [DOI] [PubMed] [Google Scholar]
  5. Dickson R. B., McManaway M. E., Lippman M. E. Estrogen-induced factors of breast cancer cells partially replace estrogen to promote tumor growth. Science. 1986 Jun 20;232(4757):1540–1543. doi: 10.1126/science.3715461. [DOI] [PubMed] [Google Scholar]
  6. Djian P., Roncari A. K., Hollenberg C. H. Influence of anatomic site and age on the replication and differentiation of rat adipocyte precursors in culture. J Clin Invest. 1983 Oct;72(4):1200–1208. doi: 10.1172/JCI111075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Djian P., Roncari D. A., Hollenberg C. H. Adipocyte precursor clones vary in capacity for differentiation. Metabolism. 1985 Sep;34(9):880–883. doi: 10.1016/0026-0495(85)90114-3. [DOI] [PubMed] [Google Scholar]
  8. Evans R. M. The steroid and thyroid hormone receptor superfamily. Science. 1988 May 13;240(4854):889–895. doi: 10.1126/science.3283939. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Funder J. W. Adrenal steroids: new answers, new questions. Science. 1987 Jul 17;237(4812):236–237. doi: 10.1126/science.3603018. [DOI] [PubMed] [Google Scholar]
  10. Krakower G. R., James R. G., Arnaud C., Etienne J., Keller R. H., Kissebah A. H. Regional adipocyte precursors in the female rat. Influence of ovarian factors. J Clin Invest. 1988 Mar;81(3):641–648. doi: 10.1172/JCI113367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lau D. C., Roncari D. A., Hollenberg C. H. Release of mitogenic factors by cultured preadipocytes from massively obese human subjects. J Clin Invest. 1987 Feb;79(2):632–636. doi: 10.1172/JCI112859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Le Roith D., Shiloach J., Berelowitz M., Frohman L. A., Liotta A. S., Krieger D. T., Roth J. Are messenger molecules in microbes the ancestors of the vertebrate hormones and tissue factors? Fed Proc. 1983 Jun;42(9):2602–2607. [PubMed] [Google Scholar]
  13. Lippman M. E., Dickson R. B., Kasid A., Gelmann E., Davidson N., McManaway M., Huff K., Bronzert D., Bates S., Swain S. Autocrine and paracrine growth regulation of human breast cancer. J Steroid Biochem. 1986 Jan;24(1):147–154. doi: 10.1016/0022-4731(86)90044-0. [DOI] [PubMed] [Google Scholar]
  14. Lippman M. E., Kasid A. Role of receptors in mediating steroid hormone effects in human breast cancer. Cancer Treat Rep. 1984 Jan;68(1):265–279. [PubMed] [Google Scholar]
  15. Roncari D. A., Lau D. C., Kindler S. Exaggerated replication in culture of adipocyte precursors from massively obese persons. Metabolism. 1981 May;30(5):425–427. doi: 10.1016/0026-0495(81)90174-8. [DOI] [PubMed] [Google Scholar]
  16. Roncari D. A., Van R. L. Promotion of human adipocyte precursor replication by 17beta-estradiol in culture. J Clin Invest. 1978 Sep;62(3):503–508. doi: 10.1172/JCI109153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Roth J., LeRoith D., Shiloach J., Rosenzweig J. L., Lesniak M. A., Havrankova J. The evolutionary origins of hormones, neurotransmitters, and other extracellular chemical messengers: implications for mammalian biology. N Engl J Med. 1982 Mar 4;306(9):523–527. doi: 10.1056/NEJM198203043060907. [DOI] [PubMed] [Google Scholar]
  18. Van R. L., Bayliss C. E., Roncari D. A. Cytological and enzymological characterization of adult human adipocyte precursors in culture. J Clin Invest. 1976 Sep;58(3):699–704. doi: 10.1172/JCI108516. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Van R. L., Roncari D. A. Complete differentiation of adipocyte precursors. A culture system for studying the cellular nature of adipose tissue. Cell Tissue Res. 1978 Dec 28;195(2):317–329. doi: 10.1007/BF00236728. [DOI] [PubMed] [Google Scholar]
  20. Van R. L., Roncari D. A. Isolation of fat cell precursors from adult rat adipose tissue. Cell Tissue Res. 1977 Jul 11;181(2):197–203. doi: 10.1007/BF00219980. [DOI] [PubMed] [Google Scholar]
  21. Vignon F., Capony F., Chambon M., Freiss G., Garcia M., Rochefort H. Autocrine growth stimulation of the MCF 7 breast cancer cells by the estrogen-regulated 52 K protein. Endocrinology. 1986 Apr;118(4):1537–1545. doi: 10.1210/endo-118-4-1537. [DOI] [PubMed] [Google Scholar]
  22. Westley B., Rochefort H. A secreted glycoprotein induced by estrogen in human breast cancer cell lines. Cell. 1980 Jun;20(2):353–362. doi: 10.1016/0092-8674(80)90621-2. [DOI] [PubMed] [Google Scholar]

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