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Tandem mass spectra of 5XY→F tau. (A) Untreated 5XY→F tau contained unmodified amino acids. (B) In contrast, PN-treated 5XY→F tau has a shift of 28 mass units on Lys385 (K, gray), indicative of a formyl-lysine. Detected N-terminal (b-series) and C-terminal (y-series) ions are indicated on the spectra and peptides comprising amino acids 384–395 are depicted.
