Abstract
An artificial amphiploid from a cross between salinity-sensitive bread wheat cultivar Chinese Spring and highly tolerant Elytrigia elongata (Host) Nevski (= Agropyron elongatum Host) shows enhanced salinity tolerance relative to Chinese Spring. Poly(A)+ RNA was isolated from roots, expanding leaves, and old leaves from amphiploid and Chinese Spring plants prior to and after acclimation to high levels of NaCl in solution cultures. Two-dimensional gel electrophoresis of the in vitro translation products was used to compare these mRNA populations. The amphiploid had 10 mRNA species induced or enhanced and 8 species repressed in root tissue during acclimation to saline growth conditions. These 18 transcripts affected by salt treatment were also detected in wheat roots, but only 4 of these were similarly regulated. In Chinese Spring the acclimation to saline stress resulted in a marked change in the level of expression of 34 transcripts in root tissue; of these, 26 were detected in the amphiploid and only 6 were regulated as in the amphiploid. No differences were seen in gene expression between salt-treated and control plants in leaves and meristematic crowns and unexpanded leaves of the amphiploid.
Keywords: gene expression, mRNA, in vitro translation, stress tolerance, interspecific hybrid
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- Anderson C. W., Straus J. W., Dudock B. S. Preparation of a cell-free protein-synthesizing system from wheat germ. Methods Enzymol. 1983;101:635–644. doi: 10.1016/0076-6879(83)01044-7. [DOI] [PubMed] [Google Scholar]
- Cathala G., Savouret J. F., Mendez B., West B. L., Karin M., Martial J. A., Baxter J. D. A method for isolation of intact, translationally active ribonucleic acid. DNA. 1983;2(4):329–335. doi: 10.1089/dna.1983.2.329. [DOI] [PubMed] [Google Scholar]
- Duncan R., Hershey J. W. Evaluation of isoelectric focusing running conditions during two-dimensional isoelectric focusing/sodium dodecyl sulfate-polyacrylamide gel electrophoresis: variation of gel patterns with changing conditions and optimized isoelectric focusing conditions. Anal Biochem. 1984 Apr;138(1):144–155. doi: 10.1016/0003-2697(84)90783-8. [DOI] [PubMed] [Google Scholar]
- Fromm M. E., Taylor L. P., Walbot V. Stable transformation of maize after gene transfer by electroporation. 1986 Feb 27-Mar 5Nature. 319(6056):791–793. doi: 10.1038/319791a0. [DOI] [PubMed] [Google Scholar]
- O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
- Ramagopal S. Differential mRNA transcription during salinity stress in barley. Proc Natl Acad Sci U S A. 1987 Jan;84(1):94–98. doi: 10.1073/pnas.84.1.94. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schöffl F., Key J. L. An analysis of mRNAs for a group of heat shock proteins of soybean using cloned cDNAs. J Mol Appl Genet. 1982;1(4):301–314. [PubMed] [Google Scholar]
- Singh N. K., Handa A. K., Hasegawa P. M., Bressan R. A. Proteins Associated with Adaptation of Cultured Tobacco Cells to NaCl. Plant Physiol. 1985 Sep;79(1):126–137. doi: 10.1104/pp.79.1.126. [DOI] [PMC free article] [PubMed] [Google Scholar]