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. 1987 Jan;84(2):493–497. doi: 10.1073/pnas.84.2.493

Genes for the catabolism and synthesis of an opine-like compound in Rhizobium meliloti are closely linked and on the Sym plasmid

Peter J Murphy *,†, Nina Heycke *, Zsofia Banfalvi ‡, Max E Tate §, Frans de Bruijn *, Adam Kondorosi ‡, Jacques Tempé , Jeff Schell *
PMCID: PMC304235  PMID: 16593802

Abstract

In alfalfa nodules induced by Rhizobium meliloti strain L5-30 the compound L-3-O-methyl-scyllo-inosamine (3-O-MSI) is synthesized. This compound is also catabolized specifically by this strain. Its biological properties are therefore similar to the Agrobacterium opines. To answer the question whether opine-like compounds (“Rhizopines”) play a role in a plant symbiotic interaction, we isolated the genes for the catabolism of 3-O-MSI (moc genes) and for the induction of its synthesis in the nodule [mos gene(s)]. moc and mos genes were shown to be closely linked and located on the Sym plasmid of L5-30, suggesting that they have co-evolved and may be important in symbiosis. These genes have been cloned into a broad host-range vector that can be mobilized into other R. meliloti strains where they are expressed. The location of the mos genes in the bacteria extends the opine concept, initially developed for a plant pathological interaction, to a symbiotic one.

Keywords: symbiosis, nodule compounds, plant-bacterial interaction

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