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. 1987 Jan;84(2):527–531. doi: 10.1073/pnas.84.2.527

Two different biosynthetic pathways for the secretion of Qa region-associated class I antigens by mouse lymphocytes.

P J Robinson
PMCID: PMC304242  PMID: 3491993

Abstract

Treatment of supernates of labeled C57BL/6 mouse lymphocytes with antibodies against beta 2-microglobulin reveals the presence of two different soluble class I molecules. One molecule (Mr, 37,000) is found in supernates of both 125I surface-labeled and [35S]methionine biosynthetically labeled cells and reacts with antibodies against Qa-2 antigens. The other molecule (Mr, 42,000) is found labeled only in supernates of [35S]methionine-labeled cells and reacts with antibodies against Qb-1. Analysis of mutant and recombinant mouse strains demonstrates that both soluble class I molecules are encoded in the Qa region. Pulse-chase experiments show that the Qa-2 molecules are released more slowly than Qb-1. It is proposed that Qb-1 molecules are secreted directly, whereas Qa-2 is first expressed on the cell surface and then processed to a soluble form.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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