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. 1987 Feb;84(3):714–718. doi: 10.1073/pnas.84.3.714

Adenovirus type 2 endopeptidase: an unusual phosphoprotein enzyme matured by autocatalysis.

P K Chatterjee, S J Flint
PMCID: PMC304286  PMID: 3543935

Abstract

A 19-kDa protein, present in low copy number in purified adenovirus type 2, has been characterized. Several criteria were used to establish that this protein is neither a degradation product of the known structural proteins of the virion nor a minor, unusually modified, form of protein VII. This 19-kDa protein, unlike other virion proteins, possesses alkali-resistant phosphoamino acids. Analysis by partial proteolysis indicated that it is related to a 23-kDa phosphoprotein present in H2ts-1 virions assembled in infected cells maintained at 39 degrees C. Affinity labeling with [3H]diisopropyl fluorophosphate showed that the 19-kDa protein contains the active site for a serine protease. We, therefore, conclude that the 19-kDa protein is the active form of the adenovirus-encoded endopeptidase, defined by the H2ts-1 mutation, and is synthesized as a 23-kDa precursor that appears to mature by autocatalysis.

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These references are in PubMed. This may not be the complete list of references from this article.

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