Abstract
To determine the phylogenetic relationships among hominoids and the dates of their divergence, the complete nucleotide sequences of the constant region of the immunoglobulin epsilon-chain (C epsilon 1) genes from chimpanzee and orangutan have been determined. These sequences were compared with the human epsilon-chain constant-region sequence. A molecular clock (silent molecular clock), measured by the degree of sequence divergence at the synonymous (silent) positions of protein-encoding regions, was introduced for the present study. From the comparison of nucleotide sequences of alpha1-antitrypsin and beta- and delta-globin genes between humans and Old World monkeys, the silent molecular clock was calibrated: the mean evolutionary rate of silent substitution was determined to be 1.56 X 10(-9) substitutions per site per year. Using the silent molecular clock, the mean divergence dates of chimpanzee and orangutan from the human lineage were estimated as 6.4 +/- 2.6 million years and 17.3 +/- 4.5 million years, respectively. It was also shown that the evolutionary rate of primate genes is considerably slower than those of other mammalian genes.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Andrews P., Cronin J. E. The relationships of Sivapithecus and Ramapithecus and the evolution of the orang-utan. Nature. 1982 Jun 17;297(5867):541–546. doi: 10.1038/297541a0. [DOI] [PubMed] [Google Scholar]
- Andrews P. Improved timing of hominoid evolution with a DNA clock. Nature. 1985 Apr 11;314(6011):498–499. doi: 10.1038/314498a0. [DOI] [PubMed] [Google Scholar]
- Battey J., Max E. E., McBride W. O., Swan D., Leder P. A processed human immunoglobulin epsilon gene has moved to chromosome 9. Proc Natl Acad Sci U S A. 1982 Oct;79(19):5956–5960. doi: 10.1073/pnas.79.19.5956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brown W. M., George M., Jr, Wilson A. C. Rapid evolution of animal mitochondrial DNA. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1967–1971. doi: 10.1073/pnas.76.4.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brown W. M., Prager E. M., Wang A., Wilson A. C. Mitochondrial DNA sequences of primates: tempo and mode of evolution. J Mol Evol. 1982;18(4):225–239. doi: 10.1007/BF01734101. [DOI] [PubMed] [Google Scholar]
- Busslinger M., Rusconi S., Birnstiel M. L. An unusual evolutionary behaviour of a sea urchin histone gene cluster. EMBO J. 1982;1(1):27–33. doi: 10.1002/j.1460-2075.1982.tb01119.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dickerson R. E. The structures of cytochrome c and the rates of molecular evolution. J Mol Evol. 1971;1(1):26–45. doi: 10.1007/BF01659392. [DOI] [PubMed] [Google Scholar]
- Goodman M., Koop B. F., Czelusniak J., Weiss M. L. The eta-globin gene. Its long evolutionary history in the beta-globin gene family of mammals. J Mol Biol. 1984 Dec 25;180(4):803–823. doi: 10.1016/0022-2836(84)90258-4. [DOI] [PubMed] [Google Scholar]
- Koop B. F., Goodman M., Xu P., Chan K., Slightom J. L. Primate eta-globin DNA sequences and man's place among the great apes. Nature. 1986 Jan 16;319(6050):234–238. doi: 10.1038/319234a0. [DOI] [PubMed] [Google Scholar]
- Kurachi K., Chandra T., Degen S. J., White T. T., Marchioro T. L., Woo S. L., Davie E. W. Cloning and sequence of cDNA coding for alpha 1-antitrypsin. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6826–6830. doi: 10.1073/pnas.78.11.6826. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lawn R. M., Efstratiadis A., O'Connell C., Maniatis T. The nucleotide sequence of the human beta-globin gene. Cell. 1980 Oct;21(3):647–651. doi: 10.1016/0092-8674(80)90428-6. [DOI] [PubMed] [Google Scholar]
- Martin S. L., Vincent K. A., Wilson A. C. Rise and fall of the delta globin gene. J Mol Biol. 1983 Mar 15;164(4):513–528. doi: 10.1016/0022-2836(83)90048-7. [DOI] [PubMed] [Google Scholar]
- Max E. E., Battey J., Ney R., Kirsch I. R., Leder P. Duplication and deletion in the human immunoglobulin epsilon genes. Cell. 1982 Jun;29(2):691–699. doi: 10.1016/0092-8674(82)90185-4. [DOI] [PubMed] [Google Scholar]
- Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
- Messing J. New M13 vectors for cloning. Methods Enzymol. 1983;101:20–78. doi: 10.1016/0076-6879(83)01005-8. [DOI] [PubMed] [Google Scholar]
- Miyata T., Hayashida H., Kikuno R., Toh H., Kawade Y. Evolution of interferon genes. Interferon. 1985;6:1–30. [PubMed] [Google Scholar]
- Miyata T., Yasunaga T. Molecular evolution of mRNA: a method for estimating evolutionary rates of synonymous and amino acid substitutions from homologous nucleotide sequences and its application. J Mol Evol. 1980 Sep;16(1):23–36. doi: 10.1007/BF01732067. [DOI] [PubMed] [Google Scholar]
- Miyata T., Yasunaga T., Nishida T. Nucleotide sequence divergence and functional constraint in mRNA evolution. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7328–7332. doi: 10.1073/pnas.77.12.7328. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nikaido T., Yamawaki-Kataoka Y., Honjo T. Nucleotide sequences of switch regions of immunoglobulin C epsilon and C gamma genes and their comparison. J Biol Chem. 1982 Jul 10;257(13):7322–7329. [PubMed] [Google Scholar]
- Rosenberg S., Barr P. J., Najarian R. C., Hallewell R. A. Synthesis in yeast of a functional oxidation-resistant mutant of human alpha-antitrypsin. Nature. 1984 Nov 1;312(5989):77–80. doi: 10.1038/312077a0. [DOI] [PubMed] [Google Scholar]
- Sarich V. M., Wilson A. C. Immunological time scale for hominid evolution. Science. 1967 Dec 1;158(3805):1200–1203. doi: 10.1126/science.158.3805.1200. [DOI] [PubMed] [Google Scholar]
- Sarich V. M., Wilson A. C. Rates of albumin evolution in primates. Proc Natl Acad Sci U S A. 1967 Jul;58(1):142–148. doi: 10.1073/pnas.58.1.142. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sibley C. G., Ahlquist J. E. The phylogeny of the hominoid primates, as indicated by DNA-DNA hybridization. J Mol Evol. 1984;20(1):2–15. doi: 10.1007/BF02101980. [DOI] [PubMed] [Google Scholar]
- Spritz R. A., DeRiel J. K., Forget B. G., Weissman S. M. Complete nucleotide sequence of the human delta-globin gene. Cell. 1980 Oct;21(3):639–646. doi: 10.1016/0092-8674(80)90427-4. [DOI] [PubMed] [Google Scholar]
- Ueda S., Nakai S., Nishida Y., Hisajima H., Honjo T. Long terminal repeat-like elements flank a human immunoglobulin epsilon pseudogene that lacks introns. EMBO J. 1982;1(12):1539–1544. doi: 10.1002/j.1460-2075.1982.tb01352.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ueda S., Takenaka O., Honjo T. A truncated immunoglobulin epsilon pseudogene is found in gorilla and man but not in chimpanzee. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3712–3715. doi: 10.1073/pnas.82.11.3712. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilson A. C., Carlson S. S., White T. J. Biochemical evolution. Annu Rev Biochem. 1977;46:573–639. doi: 10.1146/annurev.bi.46.070177.003041. [DOI] [PubMed] [Google Scholar]
- Wu C. I., Li W. H. Evidence for higher rates of nucleotide substitution in rodents than in man. Proc Natl Acad Sci U S A. 1985 Mar;82(6):1741–1745. doi: 10.1073/pnas.82.6.1741. [DOI] [PMC free article] [PubMed] [Google Scholar]