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. 1987 Mar;84(5):1439–1443. doi: 10.1073/pnas.84.5.1439

Depolarization-induced Ca2+ increase in isolated neurosecretory nerve terminals measured with fura-2.

D Brethes, G Dayanithi, L Letellier, J J Nordmann
PMCID: PMC304446  PMID: 3469676

Abstract

The free Ca2+ concentration in isolated rat neurohypophysial nerve endings was measured using the Ca2+ indicator fura-2. Depolarization with high K, veratridine, or electrical stimulation induced an increase in intracellular Ca2+ concentration that was abolished by agents known to block voltage-sensitive Ca channels. Electrical stimulation of the isolated nerve endings with a pulse pattern similar to that recorded in vivo from the hypothalamic magnocellular neurons showed that the increase in intracellular Ca2+ concentration was not only a function of the applied frequency but also of the duration of the silent interburst intervals. The relationship between the cytoplasmic free Ca concentration and the release of neuropeptides is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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