Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1987 Mar;84(6):1492–1496. doi: 10.1073/pnas.84.6.1492

Differential regulation of a Thy-1 gene in transgenic mice.

G Kollias, E Spanopoulou, F Grosveld, M Ritter, J Beech, R Morris
PMCID: PMC304460  PMID: 2882505

Abstract

We have generated Thy-1.1-transgenic Thy-1.2 mice to study the developmental expression of the Thy-1 gene in detail by transcriptional and immunological methods. In brain, the expression of the injected gene was identical to that of the endogenous gene in a tissue- and development-specific manner. In lymphoid tissue, the transferred gene was also expressed correctly in the early phases of T-cell lineage development; however, as the T cells matured, the transcription of the transferred gene, but not the endogenous gene, was suppressed. This result shows that different regulatory elements are used to express the Thy-1 gene in early and late lymphoid development.

Full text

PDF
1492

Images in this article

1493Image
on p.1493
1493Image
on p.1493
1493Image
on p.1493
1493Image
on p.1493
1493Image
on p.1493
1493Image
on p.1493
1494Image
on p.1494
1494Image
on p.1494
1495Image
on p.1495
1495Image
on p.1495
1495Image
on p.1495
1495Image
on p.1495
1495Image
on p.1495
1495Image
on p.1495
1495Image
on p.1495
1495Image
on p.1495
1495Image
on p.1495
1495Image
on p.1495
1495Image
on p.1495
1495Image
on p.1495
1495Image
on p.1495
1495Image
on p.1495
1495Image
on p.1495
1495Image
on p.1495
1495Image
on p.1495
1495Image
on p.1495
1495Image
on p.1495
1495Image
on p.1495

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beech J. N., Morris R. J., Raisman G. Density of Thy-1 on axonal membrane of different rat nerves. J Neurochem. 1983 Aug;41(2):411–417. doi: 10.1111/j.1471-4159.1983.tb04757.x. [DOI] [PubMed] [Google Scholar]
  2. Bird A. P. CpG-rich islands and the function of DNA methylation. Nature. 1986 May 15;321(6067):209–213. doi: 10.1038/321209a0. [DOI] [PubMed] [Google Scholar]
  3. Bolin L. M., Rouse R. V. Localization of Thy-1 expression during postnatal development of the mouse cerebellar cortex. J Neurocytol. 1986 Feb;15(1):29–36. doi: 10.1007/BF02057902. [DOI] [PubMed] [Google Scholar]
  4. Brinster R. L., Chen H. Y., Trumbauer M. E., Yagle M. K., Palmiter R. D. Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs. Proc Natl Acad Sci U S A. 1985 Jul;82(13):4438–4442. doi: 10.1073/pnas.82.13.4438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cobbold S. P., Jayasuriya A., Nash A., Prospero T. D., Waldmann H. Therapy with monoclonal antibodies by elimination of T-cell subsets in vivo. Nature. 1984 Dec 6;312(5994):548–551. doi: 10.1038/312548a0. [DOI] [PubMed] [Google Scholar]
  6. Giguére V., Isobe K., Grosveld F. Structure of the murine Thy-1 gene. EMBO J. 1985 Aug;4(8):2017–2024. doi: 10.1002/j.1460-2075.1985.tb03886.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ingraham H. A., Evans G. A. Characterization of two atypical promoters and alternate mRNA processing in the mouse Thy-1.2 glycoprotein gene. Mol Cell Biol. 1986 Aug;6(8):2923–2931. doi: 10.1128/mcb.6.8.2923. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ingraham H. A., Lawless G. M., Evans G. A. The mouse Thy-1.2 glycoprotein gene: complete sequence and identification of an unusual promoter. J Immunol. 1986 Feb 15;136(4):1482–1489. [PubMed] [Google Scholar]
  9. Isobe K., Kollias G., Kolsto A. B., Grosveld F. T cell independent Thy-1 allo-antibody response with the use of transgenic mice. J Immunol. 1986 Oct 1;137(7):2089–2092. [PubMed] [Google Scholar]
  10. Kollias G., Wrighton N., Hurst J., Grosveld F. Regulated expression of human A gamma-, beta-, and hybrid gamma beta-globin genes in transgenic mice: manipulation of the developmental expression patterns. Cell. 1986 Jul 4;46(1):89–94. doi: 10.1016/0092-8674(86)90862-7. [DOI] [PubMed] [Google Scholar]
  11. Kroczek R. A., Gunter K. C., Germain R. N., Shevach E. M. Thy-1 functions as a signal transduction molecule in T lymphocytes and transfected B lymphocytes. Nature. 1986 Jul 10;322(6075):181–184. doi: 10.1038/322181a0. [DOI] [PubMed] [Google Scholar]
  12. Ledbetter J. A., Herzenberg L. A. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63–90. doi: 10.1111/j.1600-065x.1979.tb00289.x. [DOI] [PubMed] [Google Scholar]
  13. Linial M., Gunderson N., Groudine M. Enhanced transcription of c-myc in bursal lymphoma cells requires continuous protein synthesis. Science. 1985 Dec 6;230(4730):1126–1132. doi: 10.1126/science.2999973. [DOI] [PubMed] [Google Scholar]
  14. Mason D. W., Williams A. F. The kinetics of antibody binding to membrane antigens in solution and at the cell surface. Biochem J. 1980 Apr 1;187(1):1–20. doi: 10.1042/bj1870001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McKenzie J. L., Fabre J. W. Human thy-1: unusual localization and possible functional significance in lymphoid tissues. J Immunol. 1981 Mar;126(3):843–850. [PubMed] [Google Scholar]
  16. Morris R. J., Barber P. C., Beech J., Raisman G. The distribution of Thy-1 antigen in the P.N.S. of the adult rat. J Neurocytol. 1983 Dec;12(6):1017–1039. doi: 10.1007/BF01153348. [DOI] [PubMed] [Google Scholar]
  17. Morris R. J., Williams A. F. Antigens on mouse and rat lymphocytes recognized by rabbit antiserum against rat brain: the quantitative analysis of a xenogeneic antiserum. Eur J Immunol. 1975 Apr;5(4):274–281. doi: 10.1002/eji.1830050412. [DOI] [PubMed] [Google Scholar]
  18. Morris R. Thy-1 in developing nervous tissue. Dev Neurosci. 1985;7(3):133–160. doi: 10.1159/000112283. [DOI] [PubMed] [Google Scholar]
  19. Muller-Sieburg C. E., Whitlock C. A., Weissman I. L. Isolation of two early B lymphocyte progenitors from mouse marrow: a committed pre-pre-B cell and a clonogenic Thy-1-lo hematopoietic stem cell. Cell. 1986 Feb 28;44(4):653–662. doi: 10.1016/0092-8674(86)90274-6. [DOI] [PubMed] [Google Scholar]
  20. REIF A. E., ALLEN J. M. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med. 1964 Sep 1;120:413–433. doi: 10.1084/jem.120.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Raff M. C. Surface antigenic markers for distinguishing T and B lymphocytes in mice. Transplant Rev. 1971;6:52–80. doi: 10.1111/j.1600-065x.1971.tb00459.x. [DOI] [PubMed] [Google Scholar]
  22. Reif A. E., Allen J. M. Mouse thymic iso-antigens. Nature. 1966 Jan 29;209(5022):521–523. doi: 10.1038/209521b0. [DOI] [PubMed] [Google Scholar]
  23. Ritter M. A., Morris R. J., Goldschneider I. Hidden Thy-1 antigen in a subpopulation of mouse bone marrow cells. Immunology. 1980 Mar;39(3):375–384. [PMC free article] [PubMed] [Google Scholar]
  24. Ritter M. A., Sauvage C. A., Delia D. Human Thy-1 antigen: cell surface expression on early T and B lymphocytes. Immunology. 1983 Jul;49(3):555–564. [PMC free article] [PubMed] [Google Scholar]
  25. Seki T., Spurr N., Obata F., Goyert S., Goodfellow P., Silver J. The human Thy-1 gene: structure and chromosomal location. Proc Natl Acad Sci U S A. 1985 Oct;82(19):6657–6661. doi: 10.1073/pnas.82.19.6657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  27. Williams A. F., Gagnon J. Neuronal cell Thy-1 glycoprotein: homology with immunoglobulin. Science. 1982 May 14;216(4547):696–703. doi: 10.1126/science.6177036. [DOI] [PubMed] [Google Scholar]
  28. Williams A. F. Immunoglobulin-related domains for cell surface recognition. Nature. 1985 Apr 18;314(6012):579–580. doi: 10.1038/314579a0. [DOI] [PubMed] [Google Scholar]
  29. van Rijs J., Giguère V., Hurst J., van Agthoven T., Geurts van Kessel A., Goyert S., Grosveld F. Chromosomal localization of the human Thy-1 gene. Proc Natl Acad Sci U S A. 1985 Sep;82(17):5832–5835. doi: 10.1073/pnas.82.17.5832. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES