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. 1987 Apr;84(7):1906–1910. doi: 10.1073/pnas.84.7.1906

Cloning of genes developmentally regulated during plant embryogenesis

Jung H Choi 1,2,*, Liang-shi Liu 1,2,†, Chumpol Borkird 1,2, Z R Sung 1,2
PMCID: PMC304550  PMID: 16593822

Abstract

Genes specifically induced during somatic embryogenesis may play key roles in plant embryo development. An antiserum against an extract of carrot somatic embryos revealed a few rare antigens induced at the onset of embryogenesis. Through differential immunoadsorption techniques, we purified antibodies against the embryo-specific antigens and probed a phage λ gt11 library of cDNA from carrot somatic embryos. This paper describes three distinct cDNA clones that hybridize to embryo-specific RNAs. Monospecific antibodies, purified by affinity to the recombinant phage fusion proteins, confirm that the cloned cDNAs encode unique embryo-specific peptide antigens. One 50-kDa protein correlates with embryogenic ability in cultures of other plant species, including cereals.

Keywords: somatic embryogenesis, differential immunoadsorption, expression vectors

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Selected References

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