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. 1987 Apr;84(7):1992–1996. doi: 10.1073/pnas.84.7.1992

Tandem linkage and unusual RNA splicing of the T-cell receptor beta-chain variable-region genes.

H S Chou, S J Anderson, M C Louie, S A Godambe, M R Pozzi, M A Behlke, K Huppi, D Y Loh
PMCID: PMC304569  PMID: 3470773

Abstract

The variable-region (V) genes of the murine T-cell receptor beta chain exist largely as single-element subfamilies. The V beta 5 and V beta 8 genes belong to the only two known three-member V beta subfamilies. We present studies on the linkage of these six genes and show that the genomic organization is that of alternating V beta 5 and V beta 8 genes. Our analysis suggests that these genes were tandemly duplicated, the unit of duplication being a pair of V beta 5 and V beta 8 genes. This tandem organization permits transcripts to initiate from the promoter of an unrearranged V beta located upstream of the rearranged V beta gene. These transcripts can generate functional beta-chain gene messages by novel RNA splicing of the upstream leader exon to the V beta coding exon of the downstream rearranged gene. We extend the analysis of the T-cell receptor genomic organization to include 12 V beta genes and suggest that all V beta genes are closely linked on chromosome 6. In addition, we discuss the possible implications of the close linkage of the V beta genes on the development of the T-cell receptor beta-chain gene repertoire.

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1992

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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