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. 1987 Apr;84(8):2175–2179. doi: 10.1073/pnas.84.8.2175

Identification of atrial natriuretic factor gene transcripts in the central nervous system of the rat.

D G Gardner, G P Vlasuk, J D Baxter, J C Fiddes, J A Lewicki
PMCID: PMC304611  PMID: 2951736

Abstract

Atrial natriuretic factor (ANF) gene transcripts have been identified in the hypothalamus, brainstem, and cerebral cortex of the rat. The hypothalamic transcripts are similar in overall size (approximately 1100 nucleotides), 5' terminus, 3' terminus, and nucleotide sequence to their counterparts synthesized in the cardiac atria. The 5' terminus of the hypothalamic transcripts, determined by both S1 nuclease and primer-extension analysis, was located approximately 20 nucleotides downstream from the TATAAAA sequence that is thought to dictate the start of transcription. Similar 5' termini were identified in RNA from the brainstem and cerebral cortex. The 3' termini of the hypothalamic transcripts mapped approximately 10 nucleotides downstream from each of two successive AATAAA sequences that are believed to provide the cleavage signal required for subsequent polyadenylylation. The same 3' termini were present in atrial, ventricular, lung, and pituitary RNA. To confirm the identity of the hypothalamic gene transcript, a partial-length cDNA clone encoding ANF was isolated from a hypothalamic cDNA library and sequenced. This hypothalamic clone was identical to an analogous atrial cDNA clone at each of its 612 nucleotide positions. These findings suggest that ANF peptides, previously identified in specific loci of the hypothalamus and pontine tegmentum, are synthesized locally in these tissues and support a role for this peptide in the central regulation of cardiovascular physiology.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Antunes-Rodrigues J., McCann S. M., Rogers L. C., Samson W. K. Atrial natriuretic factor inhibits dehydration- and angiotensin II-induced water intake in the conscious, unrestrained rat. Proc Natl Acad Sci U S A. 1985 Dec;82(24):8720–8723. doi: 10.1073/pnas.82.24.8720. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Antunes-Rodrigues J., McCann S. M., Samson W. K. Central administration of atrial natriuretic factor inhibits saline preference in the rat. Endocrinology. 1986 Apr;118(4):1726–1728. doi: 10.1210/endo-118-4-1726. [DOI] [PubMed] [Google Scholar]
  3. Argentin S., Nemer M., Drouin J., Scott G. K., Kennedy B. P., Davies P. L. The gene for rat atrial natriuretic factor. J Biol Chem. 1985 Apr 25;260(8):4568–4571. [PubMed] [Google Scholar]
  4. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
  6. Birnstiel M. L., Busslinger M., Strub K. Transcription termination and 3' processing: the end is in site! Cell. 1985 Jun;41(2):349–359. doi: 10.1016/s0092-8674(85)80007-6. [DOI] [PubMed] [Google Scholar]
  7. Camargo M. J., Kleinert H. D., Atlas S. A., Sealey J. E., Laragh J. H., Maack T. Ca-dependent hemodynamic and natriuretic effects of atrial extract in isolated rat kidney. Am J Physiol. 1984 Apr;246(4 Pt 2):F447–F456. doi: 10.1152/ajprenal.1984.246.4.F447. [DOI] [PubMed] [Google Scholar]
  8. Cathala G., Savouret J. F., Mendez B., West B. L., Karin M., Martial J. A., Baxter J. D. A method for isolation of intact, translationally active ribonucleic acid. DNA. 1983;2(4):329–335. doi: 10.1089/dna.1983.2.329. [DOI] [PubMed] [Google Scholar]
  9. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  10. Currie M. G., Geller D. M., Cole B. R., Boylan J. G., YuSheng W., Holmberg S. W., Needleman P. Bioactive cardiac substances: potent vasorelaxant activity in mammalian atria. Science. 1983 Jul 1;221(4605):71–73. doi: 10.1126/science.6857267. [DOI] [PubMed] [Google Scholar]
  11. Debinski W., Gutkowska J., Kuchel O., Racz K., Buu N. T., Cantin M., Genest J. ANF-like peptide(s) in the peripheral autonomic nervous system. Biochem Biophys Res Commun. 1986 Jan 14;134(1):279–284. doi: 10.1016/0006-291x(86)90559-0. [DOI] [PubMed] [Google Scholar]
  12. Gardner D. G., Deschepper C. F., Ganong W. F., Hane S., Fiddes J., Baxter J. D., Lewicki J. Extra-atrial expression of the gene for atrial natriuretic factor. Proc Natl Acad Sci U S A. 1986 Sep;83(18):6697–6701. doi: 10.1073/pnas.83.18.6697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Glembotski C. C., Wildey G. M., Gibson T. R. Molecular forms of immunoactive atrial natriuretic peptide in the rat hypothalamus and atrium. Biochem Biophys Res Commun. 1985 Jun 28;129(3):671–678. doi: 10.1016/0006-291x(85)91944-8. [DOI] [PubMed] [Google Scholar]
  14. Jacobowitz D. M., Skofitsch G., Keiser H. R., Eskay R. L., Zamir N. Evidence for the existence of atrial natriuretic factor-containing neurons in the rat brain. Neuroendocrinology. 1985 Jan;40(1):92–94. doi: 10.1159/000124058. [DOI] [PubMed] [Google Scholar]
  15. Januszewicz P., Thibault G., Garcia R., Gutkowska J., Genest J., Cantin M. Effect of synthetic atrial natriuretic factor on arginine vasopressin release by the rat hypothalamo-neurohypophysial complex in organ culture. Biochem Biophys Res Commun. 1986 Jan 29;134(2):652–658. doi: 10.1016/s0006-291x(86)80469-7. [DOI] [PubMed] [Google Scholar]
  16. McKenzie J. C., Tanaka I., Misono K. S., Inagami T. Immunocytochemical localization of atrial natriuretic factor in the kidney, adrenal medulla, pituitary, and atrium of rat. J Histochem Cytochem. 1985 Aug;33(8):828–832. doi: 10.1177/33.8.3160763. [DOI] [PubMed] [Google Scholar]
  17. Papka R. E., Traurig H. H., Wekstein M. Localization of peptides in nerve terminals in the paracervical ganglion of the rat by light and electron microscopic immunohistochemistry: enkephalin and atrial natriuretic factor. Neurosci Lett. 1985 Nov 11;61(3):285–290. doi: 10.1016/0304-3940(85)90478-1. [DOI] [PubMed] [Google Scholar]
  18. Quirion R., Dalpé M., Dam T. V. Characterization and distribution of receptors for the atrial natriuretic peptides in mammalian brain. Proc Natl Acad Sci U S A. 1986 Jan;83(1):174–178. doi: 10.1073/pnas.83.1.174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Quirion R., Dalpé M., De Lean A., Gutkowska J., Cantin M., Genest J. Atrial natriuretic factor (ANF) binding sites in brain and related structures. Peptides. 1984 Nov-Dec;5(6):1167–1172. doi: 10.1016/0196-9781(84)90183-9. [DOI] [PubMed] [Google Scholar]
  20. Samson W. K. Atrial natriuretic factor inhibits dehydration and hemorrhage-induced vasopressin release. Neuroendocrinology. 1985 Mar;40(3):277–279. doi: 10.1159/000124085. [DOI] [PubMed] [Google Scholar]
  21. Samson W. K. Dehydration-induced alterations in rat brain vasopressin and atrial natriuretic factor immunoreactivity. Endocrinology. 1985 Sep;117(3):1279–1281. doi: 10.1210/endo-117-3-1279. [DOI] [PubMed] [Google Scholar]
  22. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Saper C. B., Standaert D. G., Currie M. G., Schwartz D., Geller D. M., Needleman P. Atriopeptin-immunoreactive neurons in the brain: presence in cardiovascular regulatory areas. Science. 1985 Mar 1;227(4690):1047–1049. doi: 10.1126/science.2858127. [DOI] [PubMed] [Google Scholar]
  24. Schwartz D., Geller D. M., Manning P. T., Siegel N. R., Fok K. F., Smith C. E., Needleman P. Ser-Leu-Arg-Arg-atriopeptin III: the major circulating form of atrial peptide. Science. 1985 Jul 26;229(4711):397–400. doi: 10.1126/science.3160114. [DOI] [PubMed] [Google Scholar]
  25. Shiono S., Nakao K., Morii N., Yamada T., Itoh H., Sakamoto M., Sugawara A., Saito Y., Katsuura G., Imura H. Nature of atrial natriuretic polypeptide in rat brain. Biochem Biophys Res Commun. 1986 Mar 28;135(3):728–734. doi: 10.1016/0006-291x(86)90989-7. [DOI] [PubMed] [Google Scholar]
  26. Tanaka I., Misono K. S., Inagami T. Atrial natriuretic factor in rat hypothalamus, atria and plasma: determination by specific radioimmunoassay. Biochem Biophys Res Commun. 1984 Oct 30;124(2):663–668. doi: 10.1016/0006-291x(84)91606-1. [DOI] [PubMed] [Google Scholar]
  27. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Vuolteenaho O., Arjamaa O., Ling N. Atrial natriuretic polypeptides (ANP): rat atria store high molecular weight precursor but secrete processed peptides of 25-35 amino acids. Biochem Biophys Res Commun. 1985 May 31;129(1):82–88. doi: 10.1016/0006-291x(85)91406-8. [DOI] [PubMed] [Google Scholar]
  29. Yamanaka M., Greenberg B., Johnson L., Seilhamer J., Brewer M., Friedemann T., Miller J., Atlas S., Laragh J., Lewicki J. Cloning and sequence analysis of the cDNA for the rat atrial natriuretic factor precursor. Nature. 1984 Jun 21;309(5970):719–722. doi: 10.1038/309719a0. [DOI] [PubMed] [Google Scholar]
  30. de Bold A. J., Borenstein H. B., Veress A. T., Sonnenberg H. A rapid and potent natriuretic response to intravenous injection of atrial myocardial extract in rats. Life Sci. 1981 Jan 5;28(1):89–94. doi: 10.1016/0024-3205(81)90370-2. [DOI] [PubMed] [Google Scholar]

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