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. 1987 Apr;84(8):2297–2301. doi: 10.1073/pnas.84.8.2297

Pressure-sensitive ion channel in Escherichia coli.

B Martinac, M Buechner, A H Delcour, J Adler, C Kung
PMCID: PMC304637  PMID: 2436228

Abstract

We have used the patch-clamp electrical recording technique on giant spheroplasts of Escherichia coli and have discovered pressure-activated ion channels. The channels have the following properties: activation by slight positive or negative pressure; voltage dependence; large conductance; selectivity for anions over cations; dependence of activity on the species of permeant ions. We believe that these channels may be involved in bacterial osmoregulation and osmotaxis.

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Selected References

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  1. BRITTEN R. J., McCLURE F. T. The amino acid pool in Escherichia coli. Bacteriol Rev. 1962 Sep;26:292–335. doi: 10.1128/br.26.3.292-335.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Benz R., Schmid A., Hancock R. E. Ion selectivity of gram-negative bacterial porins. J Bacteriol. 1985 May;162(2):722–727. doi: 10.1128/jb.162.2.722-727.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bukau B., Ehrmann M., Boos W. Osmoregulation of the maltose regulon in Escherichia coli. J Bacteriol. 1986 Jun;166(3):884–891. doi: 10.1128/jb.166.3.884-891.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Conti F., Fioravanti R., Segal J. R., Stühmer W. Pressure dependence of the potassium currents of squid giant axon. J Membr Biol. 1982;69(1):35–40. doi: 10.1007/BF01871239. [DOI] [PubMed] [Google Scholar]
  5. Dargent B., Hofmann W., Pattus F., Rosenbusch J. P. The selectivity filter of voltage-dependent channels formed by phosphoporin (PhoE protein) from E. coli. EMBO J. 1986 Apr;5(4):773–778. doi: 10.1002/j.1460-2075.1986.tb04280.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Engel A., Massalski A., Schindler H., Dorset D. L., Rosenbusch J. P. Porin channel triplets merge into single outlets in Escherichia coli outer membranes. Nature. 1985 Oct 17;317(6038):643–645. doi: 10.1038/317643a0. [DOI] [PubMed] [Google Scholar]
  7. Felle H., Porter J. S., Slayman C. L., Kaback H. R. Quantitative measurements of membrane potential in Escherichia coli. Biochemistry. 1980 Jul 22;19(15):3585–3590. doi: 10.1021/bi00556a026. [DOI] [PubMed] [Google Scholar]
  8. Guharay F., Sachs F. Stretch-activated single ion channel currents in tissue-cultured embryonic chick skeletal muscle. J Physiol. 1984 Jul;352:685–701. doi: 10.1113/jphysiol.1984.sp015317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gustin M. C., Martinac B., Saimi Y., Culbertson M. R., Kung C. Ion channels in yeast. Science. 1986 Sep 12;233(4769):1195–1197. doi: 10.1126/science.2426783. [DOI] [PubMed] [Google Scholar]
  10. Hall M. N., Silhavy T. J. Genetic analysis of the major outer membrane proteins of Escherichia coli. Annu Rev Genet. 1981;15:91–142. doi: 10.1146/annurev.ge.15.120181.000515. [DOI] [PubMed] [Google Scholar]
  11. Hamill O. P., Marty A., Neher E., Sakmann B., Sigworth F. J. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 1981 Aug;391(2):85–100. doi: 10.1007/BF00656997. [DOI] [PubMed] [Google Scholar]
  12. Laimins L. A., Rhoads D. B., Epstein W. Osmotic control of kdp operon expression in Escherichia coli. Proc Natl Acad Sci U S A. 1981 Jan;78(1):464–468. doi: 10.1073/pnas.78.1.464. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Le Rudulier D., Strom A. R., Dandekar A. M., Smith L. T., Valentine R. C. Molecular biology of osmoregulation. Science. 1984 Jun 8;224(4653):1064–1068. doi: 10.1126/science.224.4653.1064. [DOI] [PubMed] [Google Scholar]
  14. Miller K. J., Kennedy E. P., Reinhold V. N. Osmotic adaptation by gram-negative bacteria: possible role for periplasmic oligosaccharides. Science. 1986 Jan 3;231(4733):48–51. doi: 10.1126/science.3941890. [DOI] [PubMed] [Google Scholar]
  15. Nakae T. Identification of the outer membrane protein of E. coli that produces transmembrane channels in reconstituted vesicle membranes. Biochem Biophys Res Commun. 1976 Aug 9;71(3):877–884. doi: 10.1016/0006-291x(76)90913-x. [DOI] [PubMed] [Google Scholar]
  16. Nakae T. Outer-membrane permeability of bacteria. Crit Rev Microbiol. 1986;13(1):1–62. doi: 10.3109/10408418609108734. [DOI] [PubMed] [Google Scholar]
  17. Nelson M. T. Interactions of divalent cations with single calcium channels from rat brain synaptosomes. J Gen Physiol. 1986 Feb;87(2):201–222. doi: 10.1085/jgp.87.2.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nikaido H. Proteins forming large channels from bacterial and mitochondrial outer membranes: porins and phage lambda receptor protein. Methods Enzymol. 1983;97:85–100. doi: 10.1016/0076-6879(83)97122-7. [DOI] [PubMed] [Google Scholar]
  19. Onitsuka M. O., Rikihisa Y., Maruyama H. B. Biochemical and topographical studies on Escherichia coli cell surface. IV. Giant spheroplast formation from a filamentous cell. J Bacteriol. 1979 May;138(2):567–574. doi: 10.1128/jb.138.2.567-574.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rolinson G. N. Effect of beta-lactam antibiotics on bacterial cell growth rate. J Gen Microbiol. 1980 Oct;120(2):317–323. doi: 10.1099/00221287-120-2-317. [DOI] [PubMed] [Google Scholar]
  21. Ruthe H. J., Adler J. Fusion of bacterial spheroplasts by electric fields. Biochim Biophys Acta. 1985 Sep 25;819(1):105–113. doi: 10.1016/0005-2736(85)90200-7. [DOI] [PubMed] [Google Scholar]
  22. Schindler H., Rosenbusch J. P. Matrix protein from Escherichia coli outer membranes forms voltage-controlled channels in lipid bilayers. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3751–3755. doi: 10.1073/pnas.75.8.3751. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tso W. W., Adler J. Negative chemotaxis in Escherichia coli. J Bacteriol. 1974 May;118(2):560–576. doi: 10.1128/jb.118.2.560-576.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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