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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Apoptosis. 2010 Dec;15(12):1425–1434. doi: 10.1007/s10495-010-0539-z

Figure 6. Expression of Dmp53 in the germline lead to loss of primordial germ cells (PGC's).

Figure 6

Gonads from third instar larvae were stained with germ cell marker, vasa (A–B; Green), anti-Adducin-like antibodies (A–B and G–H; Red), anti-phospho-histone 3 (C–D, Green), Lysotracker (E–F, Green), anti-GFP (G–H) and DAPI (C–F, Blue). A) nanos-Gal4VP16 ovary. B) Ovary from nanos-Gal4VP16; UASp- p53 fly, over-expressing of Dmp53 lead to loss pf PGC's. C) nanos-Gal4VP16. D) Ovary from nanos-Gal4VP16; UASp- p53 fly, over-expressing of Dmp53 lead to decrease in numbers of proliferating PGC's. E) Ovary from nanos-Gal4VP16. F) Ovary from nanos-Gal4VP16; UASp- p53 fly, LysoTracker positive puncta (arrows) have begun to accumulate in the ovary. G) Ovary from nanos-Gal4VP16; GFP-LC3 stained with anti-GFP. H) Ovary from nanos-Gal4VP16/UASp-p53; GFP-LC3 stained with anti-GFP.