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. 1987 Jul;84(13):4596–4600. doi: 10.1073/pnas.84.13.4596

Identification of HLA-DP polymorphism with DP alpha and DP beta probes and monoclonal antibodies: correlation with primed lymphocyte typing.

J Bodmer, W Bodmer, J Heyes, A So, S Tonks, J Trowsdale, J Young
PMCID: PMC305137  PMID: 2885841

Abstract

Thirty-four lymphoblastoid cell lines that had been previously typed for HLA-DP antigens by primed lymphocyte typing (PLT) were tested by Southern blotting and by ELISA. Using two DP beta probes and a DP alpha probe with a series of enzymes, it is possible to identify restriction fragment length polymorphism (RFLP) patterns characteristic of DPw1, -2, -3, -4, and possibly -5. ELISA typing results, based on two polymorphic DP antibodies DP11.1 and ILR1, were compared with PLT-defined and RFLP-defined types. Thus, using a range of probes and enzymes it is possible to identify DP polymorphism. The value of monoclonal antibodies for such studies is demonstrated, and the molecular data can, in some cases, pinpoint the amino acids responsible for the specificity of the monoclonal antibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Austin P., Trowsdale J., Rudd C., Bodmer W., Feldmann M., Lamb J. Functional expression of HLA-DP genes transfected into mouse fibroblasts. Nature. 1985 Jan 3;313(5997):61–64. doi: 10.1038/313061a0. [DOI] [PubMed] [Google Scholar]
  2. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  3. Heyes J., Austin P., Bodmer J., Bodmer W., Madrigal A., Mazzilli M. C., Trowsdale J. Monoclonal antibodies to HLA-DP-transfected mouse L cells. Proc Natl Acad Sci U S A. 1986 May;83(10):3417–3421. doi: 10.1073/pnas.83.10.3417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kelly A., Trowsdale J. Complete nucleotide sequence of a functional HLA-DP beta gene and the region between the DP beta 1 and DP alpha 1 genes: comparison of the 5' ends of HLA class II genes. Nucleic Acids Res. 1985 Mar 11;13(5):1607–1621. doi: 10.1093/nar/13.5.1607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Nadler L. M., Stashenko P., Hardy R., Tomaselli K. J., Yunis E. J., Schlossman S. F., Pesando J. M. Monoclonal antibody identifies a new Ia-like (p29,34) polymorphic system linked to the HLA-D/DR region. Nature. 1981 Apr 16;290(5807):591–593. doi: 10.1038/290591a0. [DOI] [PubMed] [Google Scholar]
  6. Nose Y., Nakatsuji T., Tsuji K., Reinsmoen N. L. A new HLA-DP (SB) specificity (Cp63) defined in Japanese and Caucasian cell populations by cloned PLT cells. Hum Immunol. 1985 Jul;13(3):145–149. doi: 10.1016/0198-8859(85)90007-2. [DOI] [PubMed] [Google Scholar]
  7. Royston I., Omary M. B., Trowbridge I. S. Monoclonal antibodies to a human T-cell antigen and Ia-like antigen in the characterization of lymphoid leukemia. Transplant Proc. 1981 Mar;13(1 Pt 2):761–766. [PubMed] [Google Scholar]
  8. Shaw S., Johnson A. H., Shearer G. M. Evidence for a new segregant series of B cell antigens that are encoded in the HLA-D region and that stimulate secondary allogenic proliferative and cytotoxic responses. J Exp Med. 1980 Sep 1;152(3):565–580. doi: 10.1084/jem.152.3.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  10. Trowsdale J., Lee J., Carey J., Grosveld F., Bodmer J., Bodmer W. Sequences related to HLA-DR alpha chain on human chromosome 6: restriction enzyme polymorphism detected with DC alpha chain probes. Proc Natl Acad Sci U S A. 1983 Apr;80(7):1972–1976. doi: 10.1073/pnas.80.7.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Trowsdale J., Young J. A., Kelly A. P., Austin P. J., Carson S., Meunier H., So A., Erlich H. A., Spielman R. S., Bodmer J. Structure, sequence and polymorphism in the HLA-D region. Immunol Rev. 1985 Jul;85:5–43. doi: 10.1111/j.1600-065x.1985.tb01129.x. [DOI] [PubMed] [Google Scholar]
  12. Wernet P., Shaw S., Brautbar C., Westphal E., Pawelec G. SB types of HLA-D homozygous typing cells. Immunogenetics. 1983;18(5):547–551. doi: 10.1007/BF00364396. [DOI] [PubMed] [Google Scholar]

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