Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1987 Jul;84(13):4655–4659. doi: 10.1073/pnas.84.13.4655

Distinct angiotensin II receptor in primary cultures of glial cells from rat brain.

M K Raizada, M I Phillips, F T Crews, C Sumners
PMCID: PMC305149  PMID: 3474621

Abstract

Angiotensin II (Ang-II) has profound effects on the brain. Receptors for Ang-II have been demonstrated on neurons, but no relationship between glial cells and Ang-II has been established. Glial cells (from the hypothalamus and brain stem of 1-day-old rat brains) in primary culture have been used to demonstrate the presence of specific Ang-II receptors. Binding of 125I-Ang-II to glial cultures was rapid, reversible, saturable, and specific for Ang-II. The rank order of potency of 125I-Ang-II binding was as follows: Ang-II = [sarcosine1,Ala8]Ang-II greater than [sarcosine1,Ile8]Ang-II much greater than Ang-III greater than Ang-I. Scatchard analysis revealed a homogeneous population of high-affinity (Kd = 1.1 nM) binding sites with a Bmax of 110 fmol/mg of protein. Light-microscopic autoradiography of 125I-Ang-II binding supported the kinetic data, documenting specific Ang-II receptors on the glial cells. Ang-II stimulated a dose-dependent hydrolysis of phosphatidylinositols in glial cells, an effect mediated by Ang-II receptors. However, Ang-II failed to influence [3H]norepinephrine uptake, and catecholamines failed to regulate Ang-II receptors, effects that occur in neurons. These observations demonstrate the presence of specific Ang-II receptors on the glial cells in primary cultures derived from normotensive rat brain. The receptors are kinetically similar to, but functionally distinct from, the neuronal Ang-II receptors.

Full text

PDF
4655

Images in this article

4656Image
on p.4656
4658Image
on p.4658

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clarke D. W., Boyd F. T., Jr, Kappy M. S., Raizada M. K. Insulin binds to specific receptors and stimulates 2-deoxy-D-glucose uptake in cultured glial cells from rat brain. J Biol Chem. 1984 Oct 10;259(19):11672–11675. [PubMed] [Google Scholar]
  2. Epstein A. N., Fitzsimons J. T., Rolls B. J. Drinking induced by injection of angiotensin into the rain of the rat. J Physiol. 1970 Sep;210(2):457–474. doi: 10.1113/jphysiol.1970.sp009220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Feldstein J. B., Gonzales R. A., Baker S. P., Sumners C., Crews F. T., Raizada M. K. Decreased alpha 1-adrenergic receptor-mediated inositide hydrolysis in neurons from hypertensive rat brain. Am J Physiol. 1986 Aug;251(2 Pt 1):C230–C237. doi: 10.1152/ajpcell.1986.251.2.C230. [DOI] [PubMed] [Google Scholar]
  4. Ganong W. F. The brain renin-angiotensin system. Annu Rev Physiol. 1984;46:17–31. doi: 10.1146/annurev.ph.46.030184.000313. [DOI] [PubMed] [Google Scholar]
  5. Ganten D., Hermann K., Bayer C., Unger T., Lang R. E. Angiotensin synthesis in the brain and increased turnover in hypertensive rats. Science. 1983 Aug 26;221(4613):869–871. doi: 10.1126/science.6879184. [DOI] [PubMed] [Google Scholar]
  6. Gonzales R. A., Feldstein J. B., Crews F. T., Raizada M. K. Receptor-mediated inositide hydrolysis is a neuronal response: comparison of primary neuronal and glial cultures. Brain Res. 1985 Oct 21;345(2):350–355. doi: 10.1016/0006-8993(85)91015-7. [DOI] [PubMed] [Google Scholar]
  7. Lind R. W., Swanson L. W., Ganten D. Angiotensin II immunoreactivity in the neural afferents and efferents of the subfornical organ of the rat. Brain Res. 1984 Nov 12;321(2):209–215. doi: 10.1016/0006-8993(84)90174-4. [DOI] [PubMed] [Google Scholar]
  8. Pelton E. W., 2nd, Kimelberg H. K., Shipherd S. V., Bourke R. S. Dopamine and norepinephrine uptake and metabolism by astroglial cells in culture. Life Sci. 1981 Apr 6;28(14):1655–1663. doi: 10.1016/0024-3205(81)90322-2. [DOI] [PubMed] [Google Scholar]
  9. Phillips M. I. Angiotensin in the brain. Neuroendocrinology. 1978;25(6):354–377. doi: 10.1159/000122756. [DOI] [PubMed] [Google Scholar]
  10. Raizada M. K., Muther T. F., Sumners C. Increased angiotensin II receptors in neuronal cultures from hypertensive rat brain. Am J Physiol. 1984 Nov;247(5 Pt 1):C364–C372. doi: 10.1152/ajpcell.1984.247.5.C364. [DOI] [PubMed] [Google Scholar]
  11. Raizada M. K., Phillips M. I., Gerndt J. S. Primary cultures from fetal rat brain incorporate [3H]-isoleucine and [3H]-valine into immunoprecipitable angiotensin II. Neuroendocrinology. 1983;36(1):64–67. doi: 10.1159/000123438. [DOI] [PubMed] [Google Scholar]
  12. Raizada M. K., Stenstrom B., Phillips M. I., Sumners C. Angiotensin II in neuronal cultures from brains of normotensive and hypertensive rats. Am J Physiol. 1984 Jul;247(1 Pt 1):C115–C119. doi: 10.1152/ajpcell.1984.247.1.C115. [DOI] [PubMed] [Google Scholar]
  13. Sirett N. E., McLean A. S., Bray J. J., Hubbard J. I. Distribution of angiotensin II receptors in rat brain. Brain Res. 1977 Feb 18;122(2):299–312. doi: 10.1016/0006-8993(77)90296-7. [DOI] [PubMed] [Google Scholar]
  14. Sumners C., Muther T. F., Raizada M. K. Altered norepinephrine uptake in neuronal cultures from spontaneously hypertensive rat brain. Am J Physiol. 1985 May;248(5 Pt 1):C488–C497. doi: 10.1152/ajpcell.1985.248.5.C488. [DOI] [PubMed] [Google Scholar]
  15. Sumners C., Phillips M. I., Raizada M. K. Angiotensin II stimulates changes in the norepinephrine content of primary cultures of rat brain. Neurosci Lett. 1983 Apr 29;36(3):305–309. doi: 10.1016/0304-3940(83)90017-4. [DOI] [PubMed] [Google Scholar]
  16. Sumners C., Raizada M. K. Angiotensin II stimulates norepinephrine uptake in hypothalamus-brain stem neuronal cultures. Am J Physiol. 1986 Feb;250(2 Pt 1):C236–C244. doi: 10.1152/ajpcell.1986.250.2.C236. [DOI] [PubMed] [Google Scholar]
  17. Sumners C., Raizada M. K. Catecholamine-angiotensin II receptor interaction in primary cultures of rat brain. Am J Physiol. 1984 May;246(5 Pt 1):C502–C509. doi: 10.1152/ajpcell.1984.246.5.C502. [DOI] [PubMed] [Google Scholar]
  18. Sumners C., Watkins L. L., Raizada M. K. Alpha 1-adrenergic receptor-mediated downregulation of angiotensin II receptors in neuronal cultures. J Neurochem. 1986 Oct;47(4):1117–1126. doi: 10.1111/j.1471-4159.1986.tb00729.x. [DOI] [PubMed] [Google Scholar]
  19. Wilson K. M., Sumners C., Hathaway S., Fregly M. J. Mineralocorticoids modulate central angiotensin II receptors in rats. Brain Res. 1986 Sep 10;382(1):87–96. doi: 10.1016/0006-8993(86)90114-9. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES