Abstract
Wittner, Masako K. (Presbyterian-St. Luke's Hospital, Chicago, Ill.), and James A. Hayashi. Studies of streptococcal cell walls. VII. Carbohydrate composition of group B cell walls. J. Bacteriol. 89:398–402. 1965.—Group B streptococcal cell walls contain 63% protein, 10% rhamnose, 18% hexose (mainly galactose, but also some glucose), 7% hexosamine (mainly glucosamine, but also galactosamine), and 3% muramic acid. The group and type antigens were extracted from isolated cell walls by acid treatment and enzymatic hydrolysis, and fractionated either with ethanol or on a diethylaminoethyl-cellulose column. Serological and chemical analyses of the fractions obtained in the two fractionation methods show that the group antigen is a rhamnose-rich polysaccharide and that the type antigen is rich in galactose and contains hexosamines.
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Selected References
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- BARKULIS S. S., JONES M. F. Studies of streptococcal cell walls. I. Isolation, chemical composition, and preparation of M protein. J Bacteriol. 1957 Aug;74(2):207–216. doi: 10.1128/jb.74.2.207-216.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CUMMINS C. S., HARRIS H. The chemical composition of the cell wall in some gram-positive bacteria and its possible value as a taxonomic character. J Gen Microbiol. 1956 Jul;14(3):583–600. doi: 10.1099/00221287-14-3-583. [DOI] [PubMed] [Google Scholar]
- DISCHE Z., BORENFREUND E. A spectrophotometric method for the microdetermination of hexosamines. J Biol Chem. 1950 Jun;184(2):517–522. [PubMed] [Google Scholar]
- HAYASHI J. A., BARKULIS S. S. Studies of streptococcal cell walls. III. The amino acids of the trypsin-treated cell wall. J Bacteriol. 1959 Feb;77(2):177–184. doi: 10.1128/jb.77.2.177-184.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HAYASHI J. A., WALSH G. Studies of streptococcal cell walls. VI. Effects of adjuvants on the production of type-specific antibodies to cell walls and isolated M protein. J Bacteriol. 1961 Nov;82:736–742. doi: 10.1128/jb.82.5.736-742.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KRAUSE R. M., McCARTY M. Studies on the chemical structure of the streptococcal cell wall. II. The composition of group C cell walls and chemical basis for serologic specificity of the carbohydrate moiety. J Exp Med. 1962 Jan 1;115:49–62. doi: 10.1084/jem.115.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MCCARTY M. The lysis of group A hemolytic streptococci by extracellular enzymes of Streptomyces albus. I. Production and fractionation of the lytic enzymes. J Exp Med. 1952 Dec;96(6):555–568. doi: 10.1084/jem.96.6.555. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MCCARTY M. Variation in the group-specific carbohydrate of group A streptococci. II. Studies on the chemical basis for serological specificity of the carbohydrates. J Exp Med. 1956 Nov 1;104(5):629–643. doi: 10.1084/jem.104.5.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SHOCKMAN G. D., KOLB J. J., TOENNIES G. A high speed shaker for the disruption of cells at low temperatures. Biochim Biophys Acta. 1957 Apr;24(1):203–204. doi: 10.1016/0006-3002(57)90168-3. [DOI] [PubMed] [Google Scholar]
- SLADE H. D., SLAMP W. C. Cell-wall composition and the grouping antigens of Streptococci. J Bacteriol. 1962 Aug;84:345–351. doi: 10.1128/jb.84.2.345-351.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]