Figure 5.

The nephric duct forms a (transiently) pseudostratified epithelium before ureteric bud formation. (A) Whole mount image of wild-type Nephric duct carrying Hoxb7/myr-Venus transgene at E10.0. Yellow lines indicate approximate planes of the sections at right, which show rostral ND in (B) and pseudostratified caudal ND in (C). (B and C) were stained with anti-GFP to detect myr-Venus (green) and anti-pH3 (red). C′, Hoechst nuclear stain of the section in (C). (D) Schematic diagram of the formation of pseudostratified epithelium preceding ureteric budding. The pseudostratified epithelium persists during early UB branching, but reverts to a simple epithelium before E14.5.⁵⁸ Scale bars 20 µM. Modified from reference ⁵⁸.