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Alkyne–SAM transfer onto histone H3 by SETDB1. A) Lysine methylation assays performed in the absence or presence of 1 and SETDB1. B) Lysine methylation assays conducted with SETDB2 along with SETDB1 as a control. C) Lysine methylation assays with 1 analyzed with the M5 anti-FLAG antibody to show the differential migration of the alkyne-modified substrate. D) Recombinant histone H3 was immunoprecipitated by using FLAG M2–agarose after CuAAC.
