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. Author manuscript; available in PMC: 2011 Mar 15.
Published in final edited form as: Vaccine. 2009 Jan 20;27(10):1589–1595. doi: 10.1016/j.vaccine.2008.12.058

Fig. 3.

Fig. 3

The production of antibody (IgG) to F. nucleatum. For immunization, cultured F. nucleatum and S. mutans were irradiated by UV at a total energy of 7000 J/m2. F. nucleatum lysate (20 µg) was separated by 10% SDS-PAGE and then subjected to Western blotting using sera (1:5000 dilution) obtained from irradiated F. nucleatum (N) and S. mutans (M)-immunized mice; immunoreactivity was developed using anti-mouse IgG conjugated with HRP complex and a Western lighting chemiluminescence kit. Molecular weights (kDa) are indicated.