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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Biochemistry. 2011 Feb 8;50(10):1634–1640. doi: 10.1021/bi101988s

Figure 2. Labeling of single-Cys replacements in helix X.

Figure 2

RSO vesicles containing given single-Cys LacY mutants were labeled with TMRM for 30 min at 0 °C or 25 °C in the absence or presence of αNPG as indicated. Experiments were carried out as described in Materials and Methods and in the legend to Fig. 1. TMRM-labeled (upper panels) and silver-stained (lower panels) bands corresponding to LacY in SDS-PAGE gels were imaged. αNPG is shown as a stick model. Helix X is highlighted in red.