Table 4.
Histologic differences between iron staining groups
| Histologic Features | No iron stain (N=556) | HC iron only (N=63) | RES iron only (N=91) | Mixed HC/RES iron (N=139) | P value† |
|---|---|---|---|---|---|
| Steatosis grade | p=0.170 | ||||
| 5–33% | 220 (40%) | 27 (43%) | 35 (38%) | 71 (51%) | |
| 34–66% | 190 (34%) | 24 (38%) | 29 (32%) | 35 (25%) | |
| >66% | 146 (26%) | 12 (19%) | 27 (30%) | 33 (24%) | |
| Fibrosis stage | p=0.092 | ||||
| None | 144 (26%) | 25 (40%) | 11 (12%) | 33 (24%) | |
| Mild to moderate, zone 3, perisinusoidal or portal/periportal only | 155 (28%) | 18 (28%) | 27 (30%) | 41 (29%) | |
| Zone 3 and periportal, any combination | 104 (19%) | 8 (13%) | 19 (21%) | 25 (18%) | |
| Bridging | 108 (19%) | 10 (16%) | 24 (26%) | 27 (19%) | |
| Cirrhosis | 45 (8%) | 2 (3%) | 10 (11%) | 13 (9%) | |
| Lobular inflammation | |||||
| 0 | 0 (0%) | 0 (0%) | 0 (0%) | 2 (1%) | p=0.110 |
| <2 under 20x | 275 (50%) | 36 (57%) | 37 (41%) | 73 (53%) | |
| 2–4 under 20x | 213 (38%) | 22 (35%) | 44 (48%) | 53 (38%) | |
| >4 under 20x | 68 (12%) | 5 (8%) | 10 (11%) | 11 (8%) | |
| Portal, chronic inflammation | |||||
| None | 81 (15%) | 9 (14%) | 10 (11%) | 23 (17%) | p=0.017 |
| Mild | 355 (64%) | 48 (76%) | 52 (57%) | 77 (55%) | |
| More than mild | 120 (21%) | 6 (10%) | 29 (32%) | 39 (28%) | |
| Hepatocellular ballooning | |||||
| None | 166 (30%) | 30 (48%) | 25 (28%) | 50 (36%) | p=0.005 |
| Mild | 136 (24%) | 18 (28%) | 22 (24%) | 42 (30%) | |
| More than mild | 254 (46%) | 15 (24%) | 44 (48%) | 47 (34%) | |
| NASH diagnosis | |||||
| No NASH | 106 (19%) | 19 (30%) | 12 (13%) | 33 (24%) | p=0.049 |
| Suspicious/borderline | 105 (19%) | 16 (25%) | 17 (19%) | 30 (21%) | |
| Definite | 345 (62%) | 28 (44%) | 62 (68%) | 76 (55%) | |
| Hepatocellular iron grade | |||||
| Absent or barely discernable, 40x | 556 (100%) | 0 (0%) | 91 (100%) | 0 (0%) | p=0.001 |
| Barely discernable granules, 20x | 0 (0%) | 52 (83%) | 0 (0%) | 80 (58%) | |
| Discrete granules resolved, 10x | 0 (0%) | 11 (17%) | 0 (0%) | 49 (35%) | |
| Discrete granules resolved, 4x | 0 (0%) | 0 (0%) | 0 (0%) | 10 (7%) | |
| RES iron grade | |||||
| None | 556 (100%) | 63 (100%) | 0 (0%) | 0 (0%) | P<0.001 |
| Mild | 0 (0%) | 0 (0%) | 74 (81%) | 79 (57%) | |
| More than mild | 0 (0%) | 0 (0%) | 17 (19%) | 60 (43%) | |
Values are N (%)
†
P-values from either Fisher’s exact or chi-square tests as appropriate.