Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1987 Jun 25;15(12):4901–4914. doi: 10.1093/nar/15.12.4901

Evidence for the involvement of the 16kD gene promoter in initiation of chromosomal replication of Escherichia coli strains carrying a B/r-derived replication origin.

N de Wind, P Parren, A R Stuitje, M Meijer
PMCID: PMC305926  PMID: 3037485

Abstract

Initiation of chromosomal DNA replication of several Escherichia coli dnaA (Ts) strains is diminished in cell harbouring pBR322 hybrid plasmids carrying both oriC and the adjacent 16kD gene promoter of E. coli K12. This perturbance, resulting in very slow growth, is caused both by the dnaA allele and the E. coli B/r-derived region of the replication origin of these strains. Cloning and DNA sequence analysis of the E. coli B/r replication origin revealed several base differences as compared to the E. coli K12 sequence. The replication origin of temperature sensitive fast growing mutants, originating from a homologous exchange between chromosomal and plasmid DNA sequences were also cloned. Sequence data showed that a single base change within the promoter of the 16kD gene of these dnaA (Ts) strains is able to suppress the inhibition of chromosomal DNA replication by the mentioned pBR322 hybrid plasmids. Our results strongly indicate a role of the 16kD gene promoter in control of initiation of chromosomal DNA replication.

Full text

PDF
4901

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beyersmann D., Messer W., Schlicht M. Mutants of Escherichia coli B-r defective in deoxyribonucleic acid initiation: dnaI, a new gene for replication. J Bacteriol. 1974 Jun;118(3):783–789. doi: 10.1128/jb.118.3.783-789.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Buhk H. J., Messer W. The replication origin region of Escherichia coli: nucleotide sequence and functional units. Gene. 1983 Oct;24(2-3):265–279. doi: 10.1016/0378-1119(83)90087-2. [DOI] [PubMed] [Google Scholar]
  3. Cleary J. M., Smith D. W., Harding N. E., Zyskind J. W. Primary structure of the chromosomal origins (oriC) of Enterobacter aerogenes and Klebsiella pneumoniae: comparisons and evolutionary relationships. J Bacteriol. 1982 Jun;150(3):1467–1471. doi: 10.1128/jb.150.3.1467-1471.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fuller R. S., Funnell B. E., Kornberg A. The dnaA protein complex with the E. coli chromosomal replication origin (oriC) and other DNA sites. Cell. 1984 Oct;38(3):889–900. doi: 10.1016/0092-8674(84)90284-8. [DOI] [PubMed] [Google Scholar]
  5. Gay N. J. Construction and characterization of an Escherichia coli strain with a uncI mutation. J Bacteriol. 1984 Jun;158(3):820–825. doi: 10.1128/jb.158.3.820-825.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hansen F. G., Koefoed S., Sørensen L., Atlung T. Titration of DnaA protein by oriC DnaA-boxes increases dnaA gene expression in Escherichia coli. EMBO J. 1987 Jan;6(1):255–258. doi: 10.1002/j.1460-2075.1987.tb04747.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hirota Y., Ryter A., Jacob F. Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division. Cold Spring Harb Symp Quant Biol. 1968;33:677–693. doi: 10.1101/sqb.1968.033.01.077. [DOI] [PubMed] [Google Scholar]
  8. Kölling R., Lother H. AsnC: an autogenously regulated activator of asparagine synthetase A transcription in Escherichia coli. J Bacteriol. 1985 Oct;164(1):310–315. doi: 10.1128/jb.164.1.310-315.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lother H., Kölling R., Kücherer C., Schauzu M. dnaA protein-regulated transcription: effects on the in vitro replication of Escherichia coli minichromosomes. EMBO J. 1985 Feb;4(2):555–560. doi: 10.1002/j.1460-2075.1985.tb03664.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Matsui M., Oka A., Takanami M., Yasuda S., Hirota Y. Sites of dnaA protein-binding in the replication origin of the Escherichia coli K-12 chromosome. J Mol Biol. 1985 Aug 5;184(3):529–533. doi: 10.1016/0022-2836(85)90299-2. [DOI] [PubMed] [Google Scholar]
  11. Meijer M., Beck E., Hansen F. G., Bergmans H. E., Messer W., von Meyenburg K., Schaller H. Nucleotide sequence of the origin of replication of the Escherichia coli K-12 chromosome. Proc Natl Acad Sci U S A. 1979 Feb;76(2):580–584. doi: 10.1073/pnas.76.2.580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Messer W. Initiation of deoxyribonucleic acid replication in Escherichia coli B-r: chronology of events and transcriptional control of initiation. J Bacteriol. 1972 Oct;112(1):7–12. doi: 10.1128/jb.112.1.7-12.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Messing J. New M13 vectors for cloning. Methods Enzymol. 1983;101:20–78. doi: 10.1016/0076-6879(83)01005-8. [DOI] [PubMed] [Google Scholar]
  14. Oka A., Sugimoto K., Takanami M., Hirota Y. Replication origin of the Escherichia coli K-12 chromosome: the size and structure of the minimum DNA segment carrying the information for autonomous replication. Mol Gen Genet. 1980 Apr;178(1):9–20. doi: 10.1007/BF00267207. [DOI] [PubMed] [Google Scholar]
  15. Rokeach L. A., Zyskind J. W. RNA terminating within the E. coli origin of replication: stringent regulation and control by DnaA protein. Cell. 1986 Aug 29;46(5):763–771. doi: 10.1016/0092-8674(86)90352-1. [DOI] [PubMed] [Google Scholar]
  16. Stuitje A. R., Meijer M. Maintenance and incompatibility of plasmids carrying the replication origin of the Escherichia coli chromosome: evidence for a control region of replication between oriC and asnA. Nucleic Acids Res. 1983 Aug 25;11(16):5775–5791. doi: 10.1093/nar/11.16.5775. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stuitje A. R., de Wind N., van der Spek J. C., Pors T. H., Meijer M. Dissection of promoter sequences involved in transcriptional activation of the Escherichia coli replication origin. Nucleic Acids Res. 1986 Mar 11;14(5):2333–2344. doi: 10.1093/nar/14.5.2333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wolf B., Newman A., Glaser D. A. On the origin and direction of replication of the Escherichia coli K12 chromosome. J Mol Biol. 1968 Mar 28;32(3):611–629. doi: 10.1016/0022-2836(68)90346-x. [DOI] [PubMed] [Google Scholar]
  19. Yamaguchi K., Yamaguchi M., Tomizawa J. Incompatibility of plasmids containing the replication origin of the Escherichia coli chromosome. Proc Natl Acad Sci U S A. 1982 Sep;79(17):5347–5351. doi: 10.1073/pnas.79.17.5347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Zyskind J. W., Cleary J. M., Brusilow W. S., Harding N. E., Smith D. W. Chromosomal replication origin from the marine bacterium Vibrio harveyi functions in Escherichia coli: oriC consensus sequence. Proc Natl Acad Sci U S A. 1983 Mar;80(5):1164–1168. doi: 10.1073/pnas.80.5.1164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. de Wind N., de Jong M., Meijer M., Stuitje A. R. Site-directed mutagenesis of the Escherichia coli chromosome near oriC: identification and characterization of asnC, a regulatory element in E. coli asparagine metabolism. Nucleic Acids Res. 1985 Dec 20;13(24):8797–8811. doi: 10.1093/nar/13.24.8797. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES