Abstract
Fluorometric titration of E. coli single-stranded DNA binding protein with various RNAs showed that the protein specifically and cooperatively binds to its own mRNA. The binding inhibited in vitro expression of ssb and bla but not nusA. This inhibition takes place at a physiological concentration of SSB. The function of the protein in gene regulation is discussed.
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- Brandsma J. A., Bosch D., de Ruÿter M., van de Putte P. Analysis of the regulatory region of the ssb gene of Escherichia coli. Nucleic Acids Res. 1985 Jul 25;13(14):5095–5109. doi: 10.1093/nar/13.14.5095. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chase J. W., Whittier R. F., Auerbach J., Sancar A., Rupp W. D. Amplification of single-strand DNA binding protein in Escherichia coli. Nucleic Acids Res. 1980 Jul 25;8(14):3215–3227. doi: 10.1093/nar/8.14.3215. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Day L. A. Circular dichroism and ultraviolet absorption of a deoxyribonucleic acid binding protein of filamentous bacteriophage. Biochemistry. 1973 Dec 18;12(26):5329–5339. doi: 10.1021/bi00750a017. [DOI] [PubMed] [Google Scholar]
- Glassberg J., Meyer R. R., Kornberg A. Mutant single-strand binding protein of Escherichia coli: genetic and physiological characterization. J Bacteriol. 1979 Oct;140(1):14–19. doi: 10.1128/jb.140.1.14-19.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Glikin G. C., Gargiulo G., Rena-Descalzi L., Worcel A. Escherichia coli single-strand binding protein stabilizes specific denatured sites in superhelical DNA. Nature. 1983 Jun 30;303(5920):770–774. doi: 10.1038/303770a0. [DOI] [PubMed] [Google Scholar]
- Johnson B. F. Genetic mapping of the lexC-113 mutation. Mol Gen Genet. 1977 Nov 29;157(1):91–97. doi: 10.1007/BF00268691. [DOI] [PubMed] [Google Scholar]
- Kuramitsu S., Hamaguchi K., Tachibana H., Horii T., Ogawa T., Ogawa H. Cysteinyl residues of Escherichia coli recA protein. Biochemistry. 1984 May 22;23(11):2363–2367. doi: 10.1021/bi00306a006. [DOI] [PubMed] [Google Scholar]
- Melton D. A., Krieg P. A., Rebagliati M. R., Maniatis T., Zinn K., Green M. R. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 1984 Sep 25;12(18):7035–7056. doi: 10.1093/nar/12.18.7035. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meyer R. R., Glassberg J., Kornberg A. An Escherichia coli mutant defective in single-strand binding protein is defective in DNA replication. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1702–1705. doi: 10.1073/pnas.76.4.1702. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Molineux I. J., Pauli A., Gefter M. L. Physical studies of the interaction between the Escherichia coli DNA binding protein and nucleic acids. Nucleic Acids Res. 1975 Oct;2(10):1821–1837. doi: 10.1093/nar/2.10.1821. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ruyechan W. T., Wetmur J. G. Studies on the cooperative binding of the Escherichia coli DNA unwinding protein to single-stranded DNA. Biochemistry. 1975 Dec 16;14(25):5529–5534. doi: 10.1021/bi00696a023. [DOI] [PubMed] [Google Scholar]
- Sancar A., Rupp W. D. Cloning of uvrA, lexC and ssb genes of Escherichia coli. Biochem Biophys Res Commun. 1979 Sep 12;90(1):123–129. doi: 10.1016/0006-291x(79)91598-5. [DOI] [PubMed] [Google Scholar]
- Scott J. F., Eisenberg S., Bertsch L. L., Kornberg A. A mechanism of duplex DNA replication revealed by enzymatic studies of phage phi X174: catalytic strand separation in advance of replication. Proc Natl Acad Sci U S A. 1977 Jan;74(1):193–197. doi: 10.1073/pnas.74.1.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shigesada K., Tsurushita N., Matsumoto Y., Imai M. Overproduction of transcription termination factor Rho in Escherichia coli. Gene. 1984 Jul-Aug;29(1-2):199–209. doi: 10.1016/0378-1119(84)90180-x. [DOI] [PubMed] [Google Scholar]
- Shimamoto N., Utiyama H. Mechanism and role of cooperative binding of bacteriophage fd gene 5 protein to single-stranded deoxyribonucleic acid. Biochemistry. 1983 Dec 6;22(25):5869–5878. doi: 10.1021/bi00294a028. [DOI] [PubMed] [Google Scholar]
- Sigal N., Delius H., Kornberg T., Gefter M. L., Alberts B. A DNA-unwinding protein isolated from Escherichia coli: its interaction with DNA and with DNA polymerases. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3537–3541. doi: 10.1073/pnas.69.12.3537. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tomizawa J. I., Anraku N., Iwama Y. Molecular mechanisms of genetic recombination in bacteriophage. VI. A mutant defective in the joining of DNA molecules. J Mol Biol. 1966 Nov 14;21(2):247–253. doi: 10.1016/0022-2836(66)90095-7. [DOI] [PubMed] [Google Scholar]
- Weiner J. H., Bertsch L. L., Kornberg A. The deoxyribonucleic acid unwinding protein of Escherichia coli. Properties and functions in replication. J Biol Chem. 1975 Mar 25;250(6):1972–1980. [PubMed] [Google Scholar]
- Williams K. R., Spicer E. K., LoPresti M. B., Guggenheimer R. A., Chase J. W. Limited proteolysis studies on the Escherichia coli single-stranded DNA binding protein. Evidence for a functionally homologous domain in both the Escherichia coli and T4 DNA binding proteins. J Biol Chem. 1983 Mar 10;258(5):3346–3355. [PubMed] [Google Scholar]