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. 1987 Jul 24;15(14):5517–5528. doi: 10.1093/nar/15.14.5517

The use of permanganate as a sequencing reagent for identification of 5-methylcytosine residues in DNA.

E Fritzsche, H Hayatsu, G L Igloi, S Iida, H Kössel
PMCID: PMC306004  PMID: 3039459

Abstract

The use of permanganate as a reagent for DNA sequencing by chemical degradation has been studied with respect to its specificity for 5-methylcytosine residues. At weakly acidic pH and room temperature, 0.2 mM potassium permanganate reacts preferentially with thymine, 5-methylcytosine, and to a lesser extent with purine residues, while cytosine remains essentially intact. Permanganate oxidation is, therefore, a suitable DNA sequencing reaction for positive discrimination between 5-methylcytosine and unmethylated cytosine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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