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. 1987 Jul 24;15(14):5787–5801. doi: 10.1093/nar/15.14.5787

Crosslinking of elongation factor Tu to tRNA(Phe) by trans-diamminedichloroplatinum (II). Characterization of two crosslinking sites in the tRNA.

F P Wikman, P Romby, M H Metz, J Reinbolt, B F Clark, J P Ebel, C Ehresmann, B Ehresmann
PMCID: PMC306023  PMID: 3302946

Abstract

Trans-diamminedichloroplatinum (II) was used to induce reversible crosslinks between EF-Tu and Phe-tRNA(Phe) within the ternary EF-Tu/GTP/Phe-tRNA(Phe) complex. Up to 40% of the complex was specifically converted into crosslinked species. Two crosslinking sites have been unambiguously identified. The major one encompassing nucleotides 58 to 65 is located in the 3'-part of the T-stem, and the minor one encompassing nucleotides 31 to 42 includes the anticodon loop and part of the 3'-strand of the anticodon stem.

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Selected References

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