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. 1987 Aug 11;15(15):6159–6169. doi: 10.1093/nar/15.15.6159

Isolation of a sequence which maps close to the human sex determining gene.

C A Pritchard, P J Goodfellow, P N Goodfellow
PMCID: PMC306075  PMID: 3627983

Abstract

A sequence mapping close to the human sex determining gene (TDF) has been isolated from a lambda library constructed with DNA derived from a chromosome transfectant hybrid cell line. This sequence is shown to be present in the DNA of X-Y interchange males at a very high frequency and, based on these studies, it is categorised with the sequence defined by the probe, GMGY3, as the closest known Y chromosome derived marker to TDF. In contrast to GMGY3, however, this locus shares no homology with any other human chromosome. Southern blot analysis also reveals specific hybridization to the Y chromosome of other primates. It therefore defines, for the first time, a conserved and Y chromosome unique locus that is near to TDF.

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Selected References

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