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. Author manuscript; available in PMC: 2012 Apr 10.
Published in final edited form as: Virology. 2011 Feb 26;412(2):315–324. doi: 10.1016/j.virol.2011.01.017

Figure 1. Confirmation of CD63 mRNA down regulation via real-time PCR.

Figure 1

(A) MDMs (5 × 105 cells/well) or (B) U373-MAGI-CCR5 cells (1 × 105 cells/well) were plated in 24-well plates and transfected with 50 nM siRNAs (CD63, CD4, and ERBB2IP). For controls, cells were treated with AZT (1 μM) or raltegravir (20 mM). Controls also included untreated cells or cells infected with HIV-1 SX (m.o.i.= 0.02) only. Total mRNA was isolated from each well using the Qiagen RNeasy kit 48 h after transfection. Quantitative RT-PCR was used to determine relative CD63 expression levels, normalizing to GAPDH expression as an internal control.