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. 1987 Aug 25;15(16):6349–6367. doi: 10.1093/nar/15.16.6349

Generation of linear multigenome-length plasmid molecules in Bacillus subtilis.

J F Viret, J C Alonso
PMCID: PMC306110  PMID: 3114717

Abstract

Linear multigenome-length double and single stranded plasmid DNA was identified in a Bacillus subtilis ATP-dependent DNAase mutant strain (addA5) bearing plasmids pC194 or pBD95ts. Plasmid pBC30, a seg mutant of pC194, as well as some pUB110 derivatives with rearrangements external to the minimal replicon, produce high amounts of such a concatemeric DNA, even in Rec+ cells. The synthesis of this type of plasmid DNA occurs in the absence of an active plasmid-encoded Rep protein and is markedly affected in polA5 and recE4 genetic backgrounds. To account for these observations, we propose that the AddAB complex serves to prevent a sigma-type replication of plasmid DNA.

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Selected References

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