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. 1987 Aug 25;15(16):6705–6711. doi: 10.1093/nar/15.16.6705

Enzymatic synthesis of RNA oligonucleotides.

L Sharmeen, J Taylor
PMCID: PMC306133  PMID: 3628005

Abstract

Using SP6 RNA polymerase and various synthetic DNA oligomers as templates (12-65 b), we have obtained efficient synthesis of RNA transcripts without the need for either primer or promoter. Gel analysis of transcripts made from templates of known size demonstrated a predominant single species, together with some minor bands. For a given template the major band had the same 5'-nucleotide as predicted from the 3'-nucleotide of the template. This synthesis procedure makes it possible to efficiently and conveniently make labeled or unlabeled RNA from synthetic DNA oligonucleotides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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