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. Author manuscript; available in PMC: 2012 Mar 21.
Published in final edited form as: Vaccine. 2011 Feb 18;29(14):2637–2647. doi: 10.1016/j.vaccine.2011.01.038

Figure 3. Schematic diagram of in vitro decoration of gpD deficient lambda phage particles with recombinant WT gpD and gp140:gpD fusion proteins.

Figure 3

gpD-deficient bacteriophage lambda procapsids were decorated with recombinant mammalian derived WT gpD protein and gp140:gpD fusion protein. The stability of the decorated phage samples was then tested by treatment with EDTA. Exposing gpD-deficient phage to EDTA disrupts the phage capsid, resulting in loss of infectivity (EDTA sensitive). In contrast, a successfully fully decorated phage particle will remain infectious in the presence of EDTA (EDTA resistant).