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. 1987 Sep 25;15(18):7265–7282. doi: 10.1093/nar/15.18.7265

Properties of a CCAAT box-binding protein.

R A Hooft van Huijsduijnen 1, J Bollekens 1, A Dorn 1, C Benoist 1, D Mathis 1
PMCID: PMC306247  PMID: 3477778

Abstract

NF-Y is a sequence-specific DNA-binding protein that interacts with the conserved Y motif of the major histocompatibility complex class II gene, E alpha. Since it is actually a CCAAT box-binding protein, NF-Y also attaches to other promoters bearing CCAAT sequences; yet, it is neither of the previously described transcription factors, CBP or CTF/NF-1. In this report, we document the cell-type distribution and various biochemical properties of NF-Y. The most important findings are that this protein is ubiquitously distributed, that it is probably a metallo-protein, that it has a protease-resistant DNA-binding domain and that the NF-Y/E alpha-olgo complex seems extremely large (greater than 200kD). These data should prove useful in comparisons of NF-Y with other sequence-specific DNA-binding proteins; they have already provided new insights into NF-Y's structure.

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