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. 1987 Oct 12;15(19):7795–7807. doi: 10.1093/nar/15.19.7795

Promoter efficiency depends upon intragenic sequences.

A M Koltunow 1, K Gregg 1, G E Rogers 1
PMCID: PMC306308  PMID: 2444923

Abstract

Experiments concerning gene transcription in Xenopus oocytes have revealed that the efficiency of the HSV-TK promoter is dependent upon the nature of the attached gene sequences. Transcriptional efficiency of the TK promoter when attached to its own gene was 30-fold higher than that observed when the promoter was attached either to an avian keratin gene or to the chicken histone H2B gene. Furthermore, attachment of the keratin gene promoter to the TK gene resulted in a 20-fold increase in keratin promoter efficiency. It was found by subsequent experiments that the TK gene is able to exert a stimulatory influence upon attached promoter sequences. This cis effect was shown to be independent of orientation but dependent upon the distance between the DNA sequences involved.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banerji J., Rusconi S., Schaffner W. Expression of a beta-globin gene is enhanced by remote SV40 DNA sequences. Cell. 1981 Dec;27(2 Pt 1):299–308. doi: 10.1016/0092-8674(81)90413-x. [DOI] [PubMed] [Google Scholar]
  2. Boston R. S., Becwar M. R., Ryan R. D., Goldsbrough P. B., Larkins B. A., Hodges T. K. Expression from heterologous promoters in electroporated carrot protoplasts. Plant Physiol. 1987 Apr;83(4):742–746. doi: 10.1104/pp.83.4.742. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brinster R. L., Chen H. Y., Warren R., Sarthy A., Palmiter R. D. Regulation of metallothionein--thymidine kinase fusion plasmids injected into mouse eggs. Nature. 1982 Mar 4;296(5852):39–42. doi: 10.1038/296039a0. [DOI] [PubMed] [Google Scholar]
  4. Charnay P., Treisman R., Mellon P., Chao M., Axel R., Maniatis T. Differences in human alpha- and beta-globin gene expression in mouse erythroleukemia cells: the role of intragenic sequences. Cell. 1984 Aug;38(1):251–263. doi: 10.1016/0092-8674(84)90547-6. [DOI] [PubMed] [Google Scholar]
  5. Coraggio I., Compagno C., Martegani E., Ranzi B. M., Sala E., Alberghina L., Viotti A. Transcription and expression of zein sequences in yeast under natural plant or yeast promoters. EMBO J. 1986 Mar;5(3):459–465. doi: 10.1002/j.1460-2075.1986.tb04234.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. D'Andrea R. J., Coles L. S., Lesnikowski C., Tabe L., Wells J. R. Chromosomal organization of chicken histone genes: preferred associations and inverted duplications. Mol Cell Biol. 1985 Nov;5(11):3108–3115. doi: 10.1128/mcb.5.11.3108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fluhr R., Kuhlemeier C., Nagy F., Chua N. H. Organ-specific and light-induced expression of plant genes. Science. 1986 May 30;232(4754):1106–1112. doi: 10.1126/science.232.4754.1106. [DOI] [PubMed] [Google Scholar]
  8. Fujita T., Shibuya H., Ohashi T., Yamanishi K., Taniguchi T. Regulation of human interleukin-2 gene: functional DNA sequences in the 5' flanking region for the gene expression in activated T lymphocytes. Cell. 1986 Aug 1;46(3):401–405. doi: 10.1016/0092-8674(86)90660-4. [DOI] [PubMed] [Google Scholar]
  9. Gorman C. M., Moffat L. F., Howard B. H. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol. 1982 Sep;2(9):1044–1051. doi: 10.1128/mcb.2.9.1044. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Karin M., Haslinger A., Holtgreve H., Richards R. I., Krauter P., Westphal H. M., Beato M. Characterization of DNA sequences through which cadmium and glucocorticoid hormones induce human metallothionein-IIA gene. Nature. 1984 Apr 5;308(5959):513–519. doi: 10.1038/308513a0. [DOI] [PubMed] [Google Scholar]
  11. Koltunow A. M., Gregg K., Rogers G. E. Intron sequences modulate feather keratin gene transcription in Xenopus oocytes. Nucleic Acids Res. 1986 Aug 26;14(16):6375–6392. doi: 10.1093/nar/14.16.6375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  13. Maguire D. J., Day A. R., Borthwick I. A., Srivastava G., Wigley P. L., May B. K., Elliott W. H. Nucleotide sequence of the chicken 5-aminolevulinate synthase gene. Nucleic Acids Res. 1986 Feb 11;14(3):1379–1391. doi: 10.1093/nar/14.3.1379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McKnight S. L., Gavis E. R., Kingsbury R., Axel R. Analysis of transcriptional regulatory signals of the HSV thymidine kinase gene: identification of an upstream control region. Cell. 1981 Aug;25(2):385–398. doi: 10.1016/0092-8674(81)90057-x. [DOI] [PubMed] [Google Scholar]
  15. McKnight S. L., Kingsbury R. C., Spence A., Smith M. The distal transcription signals of the herpesvirus tk gene share a common hexanucleotide control sequence. Cell. 1984 May;37(1):253–262. doi: 10.1016/0092-8674(84)90321-0. [DOI] [PubMed] [Google Scholar]
  16. McKnight S. L. The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene. Nucleic Acids Res. 1980 Dec 20;8(24):5949–5964. doi: 10.1093/nar/8.24.5949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Merrill G. F., Hauschka S. D., McKnight S. L. tk Enzyme expression in differentiating muscle cells is regulated through an internal segment of the cellular tk gene. Mol Cell Biol. 1984 Sep;4(9):1777–1784. doi: 10.1128/mcb.4.9.1777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Miller T. J., Stephens D. L., Mertz J. E. Kinetics of accumulation and processing of simian virus 40 RNA in Xenopus laevis oocytes injected with simian virus 40 DNA. Mol Cell Biol. 1982 Dec;2(12):1581–1594. doi: 10.1128/mcb.2.12.1581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Moore D. D., Marks A. R., Buckley D. I., Kapler G., Payvar F., Goodman H. M. The first intron of the human growth hormone gene contains a binding site for glucocorticoid receptor. Proc Natl Acad Sci U S A. 1985 Feb;82(3):699–702. doi: 10.1073/pnas.82.3.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mous J., Stunnenberg H., Georgiev O., Birnstiel M. L. Stimulation of sea urchin H2B histone gene transcription by a chromatin-associated protein fraction depends on gene sequences downstream of the transcription start site. Mol Cell Biol. 1985 Oct;5(10):2764–2769. doi: 10.1128/mcb.5.10.2764. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Norstedt G., Palmiter R. Secretory rhythm of growth hormone regulates sexual differentiation of mouse liver. Cell. 1984 Apr;36(4):805–812. doi: 10.1016/0092-8674(84)90030-8. [DOI] [PubMed] [Google Scholar]
  22. Palmiter R. D., Brinster R. L., Hammer R. E., Trumbauer M. E., Rosenfeld M. G., Birnberg N. C., Evans R. M. Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes. Nature. 1982 Dec 16;300(5893):611–615. doi: 10.1038/300611a0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Palmiter R. D., Wilkie T. M., Chen H. Y., Brinster R. L. Transmission distortion and mosaicism in an unusual transgenic mouse pedigree. Cell. 1984 Apr;36(4):869–877. doi: 10.1016/0092-8674(84)90036-9. [DOI] [PubMed] [Google Scholar]
  24. Probst E., Kressmann A., Birnstiel M. L. Expression of sea urchin histone genes in the oocyte of Xenopus laevis. J Mol Biol. 1979 Dec 15;135(3):709–732. doi: 10.1016/0022-2836(79)90173-6. [DOI] [PubMed] [Google Scholar]
  25. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Simpson J., VAN Montagu M., Herrera-Estrella L. Photosynthesis-associated gene families: differences in response to tissue-specific and environmental factors. Science. 1986 Jul 4;233(4759):34–38. doi: 10.1126/science.233.4759.34. [DOI] [PubMed] [Google Scholar]
  27. Teeri T. H., Herrera-Estrella L., Depicker A., Van Montagu M., Palva E. T. Identification of plant promoters in situ by T-DNA-mediated transcriptional fusions to the npt-II gene. EMBO J. 1986 Aug;5(8):1755–1760. doi: 10.1002/j.1460-2075.1986.tb04423.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Walker M. D., Edlund T., Boulet A. M., Rutter W. J. Cell-specific expression controlled by the 5'-flanking region of insulin and chymotrypsin genes. Nature. 1983 Dec 8;306(5943):557–561. doi: 10.1038/306557a0. [DOI] [PubMed] [Google Scholar]
  29. Wright S., Rosenthal A., Flavell R., Grosveld F. DNA sequences required for regulated expression of beta-globin genes in murine erythroleukemia cells. Cell. 1984 Aug;38(1):265–273. doi: 10.1016/0092-8674(84)90548-8. [DOI] [PubMed] [Google Scholar]

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