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. 1987 Oct 26;15(20):8177–8193. doi: 10.1093/nar/15.20.8177

Contribution of different GC-motifs to the control of simian virus 40 late promoter activity.

M Ernoult-Lange 1, F Omilli 1, E May 1
PMCID: PMC306352  PMID: 2823225

Abstract

During the course of lytic infection the 21-bp repeat region regulates differentially the late gene expression; a mutant deleted for this region expresses late genes either to a higher level in the absence of T antigen or to a lower level in the late phase of infection as compared to wild type (23). By analysing a series of clustered point mutations generated within the GC-motifs we show that i) mutations within motifs I and II stimulate late transcription two to three-fold, suggesting that competition for transcription machinery between early-early and late promoters is mediated by these two motifs, ii) after viral replication, simultaneous mutations within motifs IV, V and VI decrease to 23% the efficiency of late transcription, indicating that these motifs are elements of the late promoter. Moreover comparison of results presented in this paper with results published by Barrera-Saldana et al. strongly suggest that late-early and late promoters are regulated in a similar manner.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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