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. 1987 Oct 26;15(20):8333–8349. doi: 10.1093/nar/15.20.8333

Guanine modification during chemical DNA synthesis.

J S Eadie 1, D S Davidson 1
PMCID: PMC306363  PMID: 3671086

Abstract

Base modification during solid-phase phosphoramidite synthesis of oligodeoxynucleotides has been investigated. We have discovered chemical modification that converts dG and dG-containing oligomers to a fluorescent form. This modification has been linked to N,N-dimethylaminopyridine (DMAP), an acylation catalyst, which can displace phosphate triester adducts at the 6-position of guanine. Further, we have found that this fluorescent intermediate can be converted in ammonium hydroxide solution to 2,6 diaminopurine deoxyribonucleoside (2,6 DAP), a potentially mutagenic nucleoside analog. We have shown that N-methylimidazole (NMI) in place of DMAP eliminates the fluorescent species and reduces 2,6 DAP contamination.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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