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. 1987 Oct 26;15(20):8467–8478. doi: 10.1093/nar/15.20.8467

Synthesis and physical characterization of DNA fragments containing N4-methylcytosine and 5-methylcytosine.

V Butkus 1, S Klimasauskas 1, L Petrauskiene 1, Z Maneliene 1, A Janulaitis 1, L E Minchenkova 1, A K Schyolkina 1
PMCID: PMC306371  PMID: 3671089

Abstract

The synthesis of N4-methyl-2'-deoxycytidine and its fully protected mononucleotide, suitable for the oligonucleotide synthesis by phosphotriester method is described. A set of octanucleotides - d(CGCGCGCG), d(CG5mCGCGCG), d(CG4mCGCGCG) and dodecanucleotides - d(GGACCCGGGTCC), d(GGA5mCCCGGGTCC), d(GGA4mCCCGGGTCC) has been synthesized in a solution. Physical characterization of the oligonucleotide duplexes by means of UV and CD spectrometry provides the evidence that 4mC similarly to 5mC favours the B--greater than Z transition, although both of these methylated cytosines inhibit the B--greater than A conformational change. N4-Methylcytosine in contrast to 5-methylcytosine reduces the DNA double helix thermal stability.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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