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. Author manuscript; available in PMC: 2012 Mar 4.
Published in final edited form as: Cell. 2011 Mar 4;144(5):689–702. doi: 10.1016/j.cell.2011.02.010

Figure 2. Parkin interacts with PARIS.

Figure 2

(A) Immunoprecipitated (IP) FLAG-PARIS interacts with MYC-Parkin, but not MYC-XIAP or the PARIS homologue, V5-ZNF398 (lane 6), n = 3.

(B) GST-pull down assay between parkin and PARIS indicates a robust interaction between parkin and PARIS, n = 4.

(C) Immunoprecipitated FLAG-PARIS interacts with WT Parkin and Parkin mutants (C431F, G430D, R275W, Q311X) in SH-SY5Y cells. Lower panel, relative binding, data = mean ± S.E.M., n = 3, *p < 0.05, Student’s t-test.

(D) Immunoblot (IB) shows that Parkin and PARIS co-immunoprecipitate in human striatum, n = 3.

(E) Parkin and PARIS co-immunoprecipitate from WT mouse ventral midbrain, but not parkin KO ventral midbrain, n = 3.

See also Figure S2.