Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1987 Nov 11;15(21):8607–8620. doi: 10.1093/nar/15.21.8607

Messenger RNAs from the E1 region of bovine papillomavirus type 1 detected in virus-infected bovine cells.

S Burnett 1, J Moreno-Lopez 1, U Pettersson 1
PMCID: PMC306394  PMID: 2825116

Abstract

Bovine papillomavirus type 1 DNA replicated to a high copy number in virus-infected bovine fibroblasts. Infected bovine cells were therefore used as a source of RNA for Northern blotting analysis to search for viral transcripts hybridizing to the E1 gene region, implicated in viral DNA replication. Cytoplasmic polyadenylated RNA preparations contained at least five different E1-region transcripts, ranging from 1200 to approximately 4500 nucleotides in length. All of these species contained sequence information from the 5'-end of the E1 open reading frame, but only the largest species included sequences from its central portion. The latter transcript is a candidate mRNA for a stimulatory replication factor (R) previously mapped by genetic experiments (1).

Full text

PDF
8607

Images in this article

8610Image
on p.8610
8612Image
on p.8612
8613Image
on p.8613
8615Image
on p.8615

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahola H., Stenlund A., Moreno-Lopez J., Pettersson U. Sequences of bovine papillomavirus type 1 DNA--functional and evolutionary implications. Nucleic Acids Res. 1983 May 11;11(9):2639–2650. doi: 10.1093/nar/11.9.2639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ahola H., Stenlund A., Moreno-López J., Pettersson U. Promoters and processing sites within the transforming region of bovine papillomavirus type 1. J Virol. 1987 Jul;61(7):2240–2244. doi: 10.1128/jvi.61.7.2240-2244.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Amtmann E., Sauer G. Bovine papilloma virus transcription: polyadenylated RNA species and assessment of the direction of transcription. J Virol. 1982 Jul;43(1):59–66. doi: 10.1128/jvi.43.1.59-66.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BLACK P. H., HARTLEY J. W., ROWE W. P., HUEBNER R. J. TRANSFORMATION OF BOVINE TISSUE CULTURE CELLS BY BOVINE PAPILLOMA VIRUS. Nature. 1963 Sep 7;199:1016–1018. doi: 10.1038/1991016a0. [DOI] [PubMed] [Google Scholar]
  5. Baker C. C., Howley P. M. Differential promoter utilization by the bovine papillomavirus in transformed cells and productively infected wart tissues. EMBO J. 1987 Apr;6(4):1027–1035. doi: 10.1002/j.1460-2075.1987.tb04855.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Berg L. J., Singh K., Botchan M. Complementation of a bovine papilloma virus low-copy-number mutant: evidence for a temporal requirement of the complementing gene. Mol Cell Biol. 1986 Mar;6(3):859–869. doi: 10.1128/mcb.6.3.859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Berg L., Lusky M., Stenlund A., Botchan M. R. Repression of bovine papilloma virus replication is mediated by a virally encoded trans-acting factor. Cell. 1986 Aug 29;46(5):753–762. doi: 10.1016/0092-8674(86)90351-x. [DOI] [PubMed] [Google Scholar]
  8. Burnett T. S., Gallimore P. H. Establishment of a human keratinocyte cell line carrying complete human papillomavirus type 1 genomes: lack of vegetative viral DNA synthesis upon keratinization. J Gen Virol. 1983 Jul;64(Pt 7):1509–1520. doi: 10.1099/0022-1317-64-7-1509. [DOI] [PubMed] [Google Scholar]
  9. Campo M. S., Spandidos D. A., Lang J., Wilkie N. M. Transcriptional control signals in the genome of bovine papillomavirus type 1. Nature. 1983 May 5;303(5912):77–80. doi: 10.1038/303077a0. [DOI] [PubMed] [Google Scholar]
  10. Chen E. Y., Howley P. M., Levinson A. D., Seeburg P. H. The primary structure and genetic organization of the bovine papillomavirus type 1 genome. Nature. 1982 Oct 7;299(5883):529–534. doi: 10.1038/299529a0. [DOI] [PubMed] [Google Scholar]
  11. Chow L. T., Reilly S. S., Broker T. R., Taichman L. B. Identification and mapping of human papillomavirus type 1 RNA transcripts recovered from plantar warts and infected epithelial cell cultures. J Virol. 1987 Jun;61(6):1913–1918. doi: 10.1128/jvi.61.6.1913-1918.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Danos O., Katinka M., Yaniv M. Human papillomavirus 1a complete DNA sequence: a novel type of genome organization among papovaviridae. EMBO J. 1982;1(2):231–236. doi: 10.1002/j.1460-2075.1982.tb01152.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Dvoretzky I., Shober R., Chattopadhyay S. K., Lowy D. R. A quantitative in vitro focus assay for bovine papilloma virus. Virology. 1980 Jun;103(2):369–375. doi: 10.1016/0042-6822(80)90195-6. [DOI] [PubMed] [Google Scholar]
  14. Georges E., Croissant O., Bonneaud N., Orth G. Physical state and transcription of the cottontail rabbit papillomavirus genome in warts and transplantable VX2 and VX7 carcinomas of domestic rabbits. J Virol. 1984 Aug;51(2):530–538. doi: 10.1128/jvi.51.2.530-538.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Heilman C. A., Engel L., Lowy D. R., Howley P. M. Virus-specific transcription in bovine papillomavirus-transformed mouse cells. Virology. 1982 May;119(1):22–34. doi: 10.1016/0042-6822(82)90061-7. [DOI] [PubMed] [Google Scholar]
  16. Kleiner E., Dietrich W., Pfister H. Differential regulation of papilloma virus early gene expression in transformed fibroblasts and carcinoma cell lines. EMBO J. 1986 Aug;5(8):1945–1950. doi: 10.1002/j.1460-2075.1986.tb04448.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lancaster W. D. Apparent lack of integration of bovine papillomavirus DNA in virus-induced equine and bovine tumor cells and virus-transformed mouse cells. Virology. 1981 Jan 30;108(2):251–255. doi: 10.1016/0042-6822(81)90433-5. [DOI] [PubMed] [Google Scholar]
  18. Lancaster W. D., Olson C. Animal papillomaviruses. Microbiol Rev. 1982 Jun;46(2):191–207. doi: 10.1128/mr.46.2.191-207.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Law M. F., Lowy D. R., Dvoretzky I., Howley P. M. Mouse cells transformed by bovine papillomavirus contain only extrachromosomal viral DNA sequences. Proc Natl Acad Sci U S A. 1981 May;78(5):2727–2731. doi: 10.1073/pnas.78.5.2727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lusky M., Berg L., Weiher H., Botchan M. Bovine papilloma virus contains an activator of gene expression at the distal end of the early transcription unit. Mol Cell Biol. 1983 Jun;3(6):1108–1122. doi: 10.1128/mcb.3.6.1108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Lusky M., Botchan M. R. A bovine papillomavirus type 1-encoded modulator function is dispensable for transient viral replication but is required for establishment of the stable plasmid state. J Virol. 1986 Nov;60(2):729–742. doi: 10.1128/jvi.60.2.729-742.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Lusky M., Botchan M. R. Characterization of the bovine papilloma virus plasmid maintenance sequences. Cell. 1984 Feb;36(2):391–401. doi: 10.1016/0092-8674(84)90232-0. [DOI] [PubMed] [Google Scholar]
  23. Lusky M., Botchan M. R. Genetic analysis of bovine papillomavirus type 1 trans-acting replication factors. J Virol. 1985 Mar;53(3):955–965. doi: 10.1128/jvi.53.3.955-965.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Moar M. H., Campo M. S., Laird H., Jarrett W. F. Persistence of non-integrated viral DNA in bovine cells transformed in vitro by bovine papillomavirus type 2. Nature. 1981 Oct 29;293(5835):749–751. doi: 10.1038/293749a0. [DOI] [PubMed] [Google Scholar]
  25. Moreno-Lopez J., Pettersson U., Dinter Z., Philipson L. Characterization of a papilloma virus from the European elk (EEPV). Virology. 1981 Jul 30;112(2):589–595. doi: 10.1016/0042-6822(81)90304-4. [DOI] [PubMed] [Google Scholar]
  26. Nakabayashi Y., Chattopadhyay S. K., Lowy D. R. The transforming function of bovine papillomavirus DNA. Proc Natl Acad Sci U S A. 1983 Oct;80(19):5832–5836. doi: 10.1073/pnas.80.19.5832. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Nasseri M., Wettstein F. O. Differences exist between viral transcripts in cottontail rabbit papillomavirus-induced benign and malignant tumors as well as non-virus-producing and virus-producing tumors. J Virol. 1984 Sep;51(3):706–712. doi: 10.1128/jvi.51.3.706-712.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Olson C., Gordon D. E., Robl M. G., Lee K. P. Oncogenicity of bovine papilloma virus. Arch Environ Health. 1969 Dec;19(6):827–837. doi: 10.1080/00039896.1969.10666938. [DOI] [PubMed] [Google Scholar]
  29. Sarver N., Gruss P., Law M. F., Khoury G., Howley P. M. Bovine papilloma virus deoxyribonucleic acid: a novel eucaryotic cloning vector. Mol Cell Biol. 1981 Jun;1(6):486–496. doi: 10.1128/mcb.1.6.486. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Schiller J. T., Vass W. C., Lowy D. R. Identification of a second transforming region in bovine papillomavirus DNA. Proc Natl Acad Sci U S A. 1984 Dec;81(24):7880–7884. doi: 10.1073/pnas.81.24.7880. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  32. Spalholz B. A., Yang Y. C., Howley P. M. Transactivation of a bovine papilloma virus transcriptional regulatory element by the E2 gene product. Cell. 1985 Aug;42(1):183–191. doi: 10.1016/s0092-8674(85)80114-8. [DOI] [PubMed] [Google Scholar]
  33. Spandidos D. A., Wilkie N. M. Host-specificities of papillomavirus, Moloney murine sarcoma virus and simian virus 40 enhancer sequences. EMBO J. 1983;2(7):1193–1199. doi: 10.1002/j.1460-2075.1983.tb01566.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Stenlund A., Bream G. L., Botchan M. R. A promoter with an internal regulatory domain is part of the origin of replication in BPV-1. Science. 1987 Jun 26;236(4809):1666–1671. doi: 10.1126/science.3037693. [DOI] [PubMed] [Google Scholar]
  35. Stenlund A., Zabielski J., Ahola H., Moreno-Lopez J., Pettersson U. Messenger RNAs from the transforming region of bovine papilloma virus type I. J Mol Biol. 1985 Apr 20;182(4):541–554. doi: 10.1016/0022-2836(85)90240-2. [DOI] [PubMed] [Google Scholar]
  36. THOMAS M., BOIRON M., TANZER J., LEVY J. P., BERNARD J. IN VITRO TRANSFORMATION OF MICE CELLS BY BOVINE PAPILLOMA VIRUS. Nature. 1964 May 16;202:709–710. doi: 10.1038/202709a0. [DOI] [PubMed] [Google Scholar]
  37. Taichman L. B., Breitburd F., Croissant O., Orth G. The search for a culture system for papillomavirus. J Invest Dermatol. 1984 Jul;83(1 Suppl):2s–6s. doi: 10.1111/1523-1747.ep12281108. [DOI] [PubMed] [Google Scholar]
  38. Yang Y. C., Okayama H., Howley P. M. Bovine papillomavirus contains multiple transforming genes. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1030–1034. doi: 10.1073/pnas.82.4.1030. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES