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. 1987 Nov 11;15(21):8983–8997. doi: 10.1093/nar/15.21.8983

Overdrive is a T-region transfer enhancer which stimulates T-strand production in Agrobacterium tumefaciens.

M J van Haaren 1, N J Sedee 1, R A Schilperoort 1, P J Hooykaas 1
PMCID: PMC306417  PMID: 3684577

Abstract

Introduction of a left or right synthetic border repeat together with the overdrive sequence in an octopine Ti-plasmid deletion mutant, lacking the right border, resulted in the complete restoration of the oncogenicity of the mutant strain. However introduction of a border repeat without the overdrive, only restored oncogenicity partially. The overdrive sequence turned out to be able to stimulate the synthetic border mediated T-region transfer, independent of its orientation and position relative to the border repeat. Furthermore the distance between border repeat and overdrive could be enlarged, without a loss of overdrive activity. Here we enlarged the distance between the two sequences up to 6714bp. These results were confirmed by estimating the amount of single stranded T-DNA molecules from induced agrobacteria, containing the various border constructs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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