Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1987 Nov 25;15(22):9451–9459. doi: 10.1093/nar/15.22.9451

The primary structure of rat ribosomal protein S8.

Y L Chan 1, A Lin 1, V Paz 1, I G Wool 1
PMCID: PMC306479  PMID: 3684599

Abstract

The amino acid sequence of rat ribosomal protein S8 was deduced from the sequence of nucleotides in a recombinant cDNA and confirmed from the NH2- and carboxyl-terminal amino acid sequences of the protein. Ribosomal protein S8 contains 207 amino acids (the NH2-terminal methionine is removed after translation of the mRNA) and has a molecular weight of 23,928. Hybridization of the cDNA to digests of nuclear DNA suggests that there are 7-9 copies of the S8 gene. Ribosomal protein S8 contains a possible internal repeat that has 12 or 13 residues, is basic, and occurs 5 times in the protein.

Full text

PDF
9451

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aoyama K., Hidaka S., Tanaka T., Ishikawa K. The nucleotide sequence of 5S RNA from rat liver ribosomes. J Biochem. 1982 Jan;91(1):363–367. doi: 10.1093/oxfordjournals.jbchem.a133696. [DOI] [PubMed] [Google Scholar]
  2. Chan Y. L., Gutell R., Noller H. F., Wool I. G. The nucleotide sequence of a rat 18 S ribosomal ribonucleic acid gene and a proposal for the secondary structure of 18 S ribosomal ribonucleic acid. J Biol Chem. 1984 Jan 10;259(1):224–230. [PubMed] [Google Scholar]
  3. Chan Y. L., Lin A., McNally J., Peleg D., Meyuhas O., Wool I. G. The primary structure of rat ribosomal protein L19. A determination from the sequence of nucleotides in a cDNA and from the sequence of amino acids in the protein. J Biol Chem. 1987 Jan 25;262(3):1111–1115. [PubMed] [Google Scholar]
  4. Chan Y. L., Olvera J., Wool I. G. The structure of rat 28S ribosomal ribonucleic acid inferred from the sequence of nucleotides in a gene. Nucleic Acids Res. 1983 Nov 25;11(22):7819–7831. doi: 10.1093/nar/11.22.7819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Collatz E., Wool I. G., Lin A., Stöffler G. The isolation of eukaryotic ribosomal proteins. The purification and characterization of the 40 S ribosomal subunit proteins S2, S3, S4, S5, S6, S7, S8, S9, S13, S23/S24, S27, and S28. J Biol Chem. 1976 Aug 10;251(15):4666–4672. [PubMed] [Google Scholar]
  6. Dingwall C., Laskey R. A. Protein import into the cell nucleus. Annu Rev Cell Biol. 1986;2:367–390. doi: 10.1146/annurev.cb.02.110186.002055. [DOI] [PubMed] [Google Scholar]
  7. Dudov K. P., Perry R. P. The gene family encoding the mouse ribosomal protein L32 contains a uniquely expressed intron-containing gene and an unmutated processed gene. Cell. 1984 Jun;37(2):457–468. doi: 10.1016/0092-8674(84)90376-3. [DOI] [PubMed] [Google Scholar]
  8. Giri L., Hill W. E., Wittmann H. G., Wittmann-Liebold B. Ribosomal proteins: their structure and spatial arrangement in prokaryotic ribosomes. Adv Protein Chem. 1984;36:1–78. doi: 10.1016/s0065-3233(08)60295-8. [DOI] [PubMed] [Google Scholar]
  9. Hadjiolov A. A., Georgiev O. I., Nosikov V. V., Yavachev L. P. Primary and secondary structure of rat 28 S ribosomal RNA. Nucleic Acids Res. 1984 Apr 25;12(8):3677–3693. doi: 10.1093/nar/12.8.3677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Itoh T., Otaka E., Matsui K. A. Primary structures of ribosomal protein YS25 from Saccharomyces cerevisiae and its counterparts from Schizosaccharomyces pombe and rat liver. Biochemistry. 1985 Dec 3;24(25):7418–7423. doi: 10.1021/bi00346a058. [DOI] [PubMed] [Google Scholar]
  11. Kalderon D., Roberts B. L., Richardson W. D., Smith A. E. A short amino acid sequence able to specify nuclear location. Cell. 1984 Dec;39(3 Pt 2):499–509. doi: 10.1016/0092-8674(84)90457-4. [DOI] [PubMed] [Google Scholar]
  12. Klein A., Meyuhas O. A multigene family of intron lacking and containing genes, encoding for mouse ribosomal protein L7. Nucleic Acids Res. 1984 May 11;12(9):3763–3776. doi: 10.1093/nar/12.9.3763. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kozak M. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell. 1986 Jan 31;44(2):283–292. doi: 10.1016/0092-8674(86)90762-2. [DOI] [PubMed] [Google Scholar]
  14. Kuwano Y., Nakanishi O., Nabeshima Y., Tanaka T., Ogata K. Molecular cloning and nucleotide sequence of DNA complementary to rat ribosomal protein S26 messenger RNA. J Biochem. 1985 Apr;97(4):983–992. doi: 10.1093/oxfordjournals.jbchem.a135175. [DOI] [PubMed] [Google Scholar]
  15. Lastick S. M., McConkey E. H. Exchange and stability of HeLa ribosomal proteins in vivo. J Biol Chem. 1976 May 25;251(10):2867–2875. [PubMed] [Google Scholar]
  16. Leer R. J., van Raamsdonk-Duin M. M., Hagendoorn M. J., Mager W. H., Planta R. J. Structural comparison of yeast ribosomal protein genes. Nucleic Acids Res. 1984 Sep 11;12(17):6685–6700. doi: 10.1093/nar/12.17.6685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lin A., McNally J., Wool I. G. The primary structure of rat liver ribosomal protein L37. Homology with yeast and bacterial ribosomal proteins. J Biol Chem. 1983 Sep 10;258(17):10664–10671. [PubMed] [Google Scholar]
  18. Lin A., McNally J., Wool I. G. The primary structure of rat liver ribosomal protein L39. J Biol Chem. 1984 Jan 10;259(1):487–490. [PubMed] [Google Scholar]
  19. Lin A., Wittmann-Liebold B., McNally J., Wool I. G. The primary structure of the acidic phosphoprotein P2 from rat liver 60 S ribosomal subunits. Comparison with ribosomal 'A' proteins from other species. J Biol Chem. 1982 Aug 10;257(15):9189–9197. [PubMed] [Google Scholar]
  20. Loreni F., Ruberti I., Bozzoni I., Pierandrei-Amaldi P., Amaldi F. Nucleotide sequence of the L1 ribosomal protein gene of Xenopus laevis: remarkable sequence homology among introns. EMBO J. 1985 Dec 16;4(13A):3483–3488. doi: 10.1002/j.1460-2075.1985.tb04107.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Monk R. J., Meyuhas O., Perry R. P. Mammals have multiple genes for individual ribosomal proteins. Cell. 1981 May;24(2):301–306. doi: 10.1016/0092-8674(81)90319-6. [DOI] [PubMed] [Google Scholar]
  22. Moreland R. B., Nam H. G., Hereford L. M., Fried H. M. Identification of a nuclear localization signal of a yeast ribosomal protein. Proc Natl Acad Sci U S A. 1985 Oct;82(19):6561–6565. doi: 10.1073/pnas.82.19.6561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Nakanishi O., Oyanagi M., Kuwano Y., Tanaka T., Nakayama T., Mitsui H., Nabeshima Y., Ogata K. Molecular cloning and nucleotide sequences of cDNAs specific for rat liver ribosomal proteins S17 and L30. Gene. 1985;35(3):289–296. doi: 10.1016/0378-1119(85)90007-1. [DOI] [PubMed] [Google Scholar]
  24. Nazar R. N., Sitz T. O., Busch H. Structural analyses of mammalian ribosomal ribonucleic acid and its precursors. Nucleotide sequence of ribosomal 5.8 S ribonucleic acid. J Biol Chem. 1975 Nov 25;250(22):8591–8597. [PubMed] [Google Scholar]
  25. Proudfoot N. J., Brownlee G. G. 3' non-coding region sequences in eukaryotic messenger RNA. Nature. 1976 Sep 16;263(5574):211–214. doi: 10.1038/263211a0. [DOI] [PubMed] [Google Scholar]
  26. Queen C. L., Korn L. J. Computer analysis of nucleic acids and proteins. Methods Enzymol. 1980;65(1):595–609. doi: 10.1016/s0076-6879(80)65062-9. [DOI] [PubMed] [Google Scholar]
  27. Tanaka T., Kuwano Y., Ishikawa K., Ogata K. Nucleotide sequence of cloned cDNA specific for rat ribosomal protein S11. J Biol Chem. 1985 May 25;260(10):6329–6333. [PubMed] [Google Scholar]
  28. Tanaka T., Kuwano Y., Kuzumaki T., Ishikawa K., Ogata K. Nucleotide sequence of cloned cDNA specific for rat ribosomal protein L31. Eur J Biochem. 1987 Jan 2;162(1):45–48. doi: 10.1111/j.1432-1033.1987.tb10539.x. [DOI] [PubMed] [Google Scholar]
  29. Tanaka T., Wakasugi K., Kuwano Y., Ishikawa K., Ogata K. Nucleotide sequence of cloned cDNA specific for rat ribosomal protein L35a. Eur J Biochem. 1986 Feb 3;154(3):523–527. doi: 10.1111/j.1432-1033.1986.tb09429.x. [DOI] [PubMed] [Google Scholar]
  30. Tas P. W., Sells B. H. Accessibility of ribosomal proteins to lactoperoxidase-catalyzed iodination following phosphorylation and during subunit interaction. Eur J Biochem. 1978 Dec 1;92(1):271–278. doi: 10.1111/j.1432-1033.1978.tb12745.x. [DOI] [PubMed] [Google Scholar]
  31. Tolan D. R., Hershey J. W., Traut R. T. Crosslinking of eukaryotic initiation factor eIF3 to the 40S ribosomal subunit from rabbit reticulocytes. Biochimie. 1983 Jul;65(7):427–436. doi: 10.1016/s0300-9084(83)80062-5. [DOI] [PubMed] [Google Scholar]
  32. Torczynski R., Bollon A. P., Fuke M. The complete nucleotide sequence of the rat 18S ribosomal RNA gene and comparison with the respective yeast and frog genes. Nucleic Acids Res. 1983 Jul 25;11(14):4879–4890. doi: 10.1093/nar/11.14.4879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Wagner M., Perry R. P. Characterization of the multigene family encoding the mouse S16 ribosomal protein: strategy for distinguishing an expressed gene from its processed pseudogene counterparts by an analysis of total genomic DNA. Mol Cell Biol. 1985 Dec;5(12):3560–3576. doi: 10.1128/mcb.5.12.3560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Westermann P., Nygård O. The spatial arrangement of the complex between eukaryotic initiation factor eIF-3 and 40 S ribosomal subunit. Cross-linking between factor and ribosomal proteins. Biochim Biophys Acta. 1983 Oct 13;741(1):103–108. doi: 10.1016/0167-4781(83)90015-5. [DOI] [PubMed] [Google Scholar]
  35. Wiedemann L. M., Perry R. P. Characterization of the expressed gene and several processed pseudogenes for the mouse ribosomal protein L30 gene family. Mol Cell Biol. 1984 Nov;4(11):2518–2528. doi: 10.1128/mcb.4.11.2518. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Wittmann-Liebold B., Geissler A. W., Lin A., Wool I. G. Sequence of the amino-terminal region of rat liver ribosomal proteins S4, S6, S8, L6, L7a, L18, L27, L30, L37, L37a, and L39. J Supramol Struct. 1979;12(4):425–433. doi: 10.1002/jss.400120403. [DOI] [PubMed] [Google Scholar]
  37. Wool I. G. The structure and function of eukaryotic ribosomes. Annu Rev Biochem. 1979;48:719–754. doi: 10.1146/annurev.bi.48.070179.003443. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES