Figure 5.

Ca²⁺ loading of red blood cells (RBCs) from sickle cell patients. RBCs were loaded with the Ca²⁺ fluorophore fluo-4 (see Methods). They were then incubated for 30 min in the absence (left—thin line) or presence (right—thick line) of bromo-A23187 at an extracellular [Ca²⁺] of 1 μM. Results are presented as histogram of fluorescence of a single experiment representative of 3.