Table 2.

Average sequencing depth for all genomic regions (high complexity and microsatellite regions) as a function of average read length

			Pilot 2 (Full Genome)			Pilot 3 (Exons of ~1,000 Genes)
Genome	Family	Relation	Short Reads	Long Reads (45bp+)	Total	Short Reads	Long Reads (45bp+)	Total
NA12878	Utah	daughter	23.8×	21.1×	44.9×	410.6×	52.6×	463.2×
NA12891	Utah	father	0.8×	21.3×	22.0×	-	233.7×	233.7×
NA12892	Utah	mother	2.6×	15.9×	18.4×	0.8×	228.4×	229.2×
NA19238	Nigeria	mother	11.5×	2.2×	13.7×	-	503.0×	503.0×
NA19239	Nigeria	father	15.2×	2.6×	17.7×	2.7×	482.6×	485.3×
NA19240	Nigeria	daughter	52.7×	11.5×	64.2×	-	791.6×	791.6×

Coverage is shown for the two kindreds for different read sizes with long reads as those which are at least 45 base pairs in length. Since pilot three was a target enrichment study, the coverage was calculated as the average coverage over the target regions. The pilot two coverage was calculated as the average effective coverage based on the size of the reads.