Delimitation of Neonectria and Cylindrocarpon (Nectriaceae, Hypocreales, Ascomycota) and related genera with Cylindrocarpon-like anamorphs

P Chaverri; C Salgado; Y Hirooka; AY Rossman; GJ Samuels

doi:10.3114/sim.2011.68.03

. 2011;68:57–78. doi: 10.3114/sim.2011.68.03

Delimitation of Neonectria and Cylindrocarpon (Nectriaceae, Hypocreales, Ascomycota) and related genera with Cylindrocarpon-like anamorphs

P Chaverri ^1,^*, C Salgado ¹, Y Hirooka ^1,², AY Rossman ², GJ Samuels ²

PMCID: PMC3065985 PMID: 21523189

Abstract

Neonectria is a cosmopolitan genus and it is, in part, defined by its link to the anamorph genus Cylindrocarpon. Neonectria has been divided into informal groups on the basis of combined morphology of anamorph and teleomorph. Previously, Cylindrocarpon was divided into four groups defined by presence or absence of microconidia and chlamydospores. Molecular phylogenetic analyses have indicated that Neonectria sensu stricto and Cylindrocarpon sensu stricto are phylogenetically congeneric. In addition, morphological and molecular data accumulated over several years have indicated that Neonectria sensu lato and Cylindrocarpon sensu lato do not form a monophyletic group and that the respective informal groups may represent distinct genera. In the present work, a multilocus analysis (act, ITS, LSU, rpb1, tef1, tub) was applied to representatives of the informal groups to determine their level of phylogenetic support as a first step towards taxonomic revision of Neonectria sensu lato. Results show five distinct highly supported clades that correspond to some extent with the informal Neonectria and Cylindrocarpon groups that are here recognised as genera: (1) N. coccinea-group and Cylindrocarpon groups 1 & 4 (Neonectria/Cylindrocarpon sensu stricto); (2) N. rugulosa-group (Rugonectria gen. nov.); (3) N. mammoidea/N. veuillotiana-groups and Cylindrocarpon group 2 (Thelonectria gen. nov.); (4) N. radicicola-group and Cylindrocarpon group 3 (Ilyonectria gen. nov.); and (5) anamorph genus Campylocarpon. Characteristics of the anamorphs and teleomorphs correlate with the five genera, three of which are newly described. New combinations are made for species where their classification is confirmed by phylogenetic data.

Keywords: Canker-causing fungi, molecular systematics, Nectria-like fungi, phylogeny, polyphasic taxonomy, root-rotting fungi, sequence analysis, systematics, taxonomy

INTRODUCTION

Species of Neonectria sensu lato and their anamorphs in Cylindrocarpon are common in tropical and temperate regions. They are generally found on bark of recently killed woody plants and sometimes on decaying herbaceous material (Samuels 1988, Samuels & Brayford 1990, Samuels et al. 1990, Samuels & Brayford 1993, 1994, Rossman et al. 1999, Castlebury et al. 2006). Some species of this genus are plant pathogens causing cankers, root rots, and other diseases on hardwood and coniferous trees, e.g. Abies and Acer cankers caused by Neonectria castaneicola; beech (Fagus) bark disease caused by N. coccinea, N. ditissima and N. faginata; black foot disease of grapevines (Vitis) caused by N. liriodendri; root rots caused by N. radicicola; and cankers caused by N. rugulosa, among others (Samuels & Brayford 1994, Hirooka et al. 2005, Kobayashi et al. 2005, Castlebury et al. 2006, Halleen et al. 2006). According to Index Fungorum (www.indexfungorum.org), 38 species have been placed in Neonectria and 143 in Cylindrocarpon. These numbers are underestimated because several species of Nectria-like fungi with Cylindrocarpon anamorphs have not been transferred to Neonectria (> 20 spp.). To date, the most comprehensive taxonomic works of Neonectria and species of Nectria having Cylindrocarpon anamorphs are those by Booth (1959, 1966) and Samuels, Brayford and collaborators (Samuels 1988, Brayford & Samuels 1993, Samuels & Brayford 1993, 1994, Brayford et al. 2004).

Species of Neonectria sensu lato are characterised by having perithecia that are subglobose to broadly obpyriform, smooth to roughened, red, becoming dark red in 3 % potassium hydroxide (KOH), and with an acute to constricted apex that is sometimes knobby; the perithecial wall is ca. 50 μm thick and generally composed of two regions, sometimes with an outer region that forms textura epidermoidea, that may or may not be covered with another region of cells; and the ascospores are hyaline, generally bicellular, rarely multi-cellular, and smooth or finely ornamented (Rossman et al. 1999). The anamorph of N. ramulariae (type of Neonectria) is Cylindrocarpon obtusiusculum and, consequently, species with the Neonectria-like morphology described above and Cylindrocarpon anamorphs have been classified as Neonectria. However, species that have been placed in Neonectria and species of Nectria having a Cylindrocarpon anamorph vary greatly in the morphology of their perithecia, some having perithecial walls < 50 μm or > 50 μm thick, others warted, and others with various degrees of ascospore ornamentation. Some species of Neonectria are morphologically similar in perithecial morphology with differences seen only in the anamorph (Samuels et al. 2006b).

The morphological variation in Neonectria resulted in the subdivision of species into five informal groups, mostly based on perithecial characteristics: (1) N. coccinea/galligena-group (Neonectria sensu stricto) (Booth 1959); (2) N. mammoidea-group (N. mammoidea = N. discophora (Booth 1959); (3) N. rugulosa-group (Samuels & Brayford 1994); (4) N. radicicola-group (Booth 1959); and (5) N. veuillotiana-group (Brayford & Samuels 1993). Species in the N. coccinea/galligena-group are characterised by having few to numerous perithecia clustered on wood; perithecial walls are ca. 50 μm thick, composed of relatively thick-walled, small cells; and ascospores are generally smooth (Booth 1959). Species in the N. mammoidea-group were originally defined as having a distinctive perithecial wall that comprises a layer of hyphae that have thickened walls and are typically arranged radially, giving the appearance of a palisade (Booth 1959, Brayford et al. 2004). This characteristic generally results in smooth, shiny perithecia. In addition to the perithecial anatomy, the N. mammoidea-group has spinulose, often yellow-brown ascospores, and a non-microconidial anamorph. The N. rugulosa-group includes species with warted perithecia, a perithecial wall > 50 μm thick, composed of large, thick-walled cells, and striate ascospores (Samuels & Brayford 1994). The N. radicicola-group includes species that have smooth to slightly warted, usually solitary perithecia, the outer region of the perithecial wall composed of large, thin-walled cells, and smooth ascospores (Samuels & Brayford 1990). Species in the N. veuillotiana-group have perithecia with a flattened or knobby apex, perithecial walls composed of thick-walled cells, and tuberculate ascospores (Brayford & Samuels 1993). Mantiri et al. (2001) revised the informal groupings of Neonectria based on phylogenetic analyses of DNA sequence data. Group or clade I was Neonectria sensu stricto or the N. coccinea/galligena-group; clade II included the N. mammoidea-, N. rugulosa-, and N. veuillotiana-groups; and clade III was the N. radicicola-group.

Booth (1966) subdivided Cylindrocarpon into four groups based on the presence or absence of microconidia and chlamydospores. The first three Cylindrocarpon groups in Booth (1966) correlate with the three groups/clades in Mantiri et al. 2001 (Castlebury et al. 2006). Anamorphs in the N. coccinea/galligena-group (clade I in Mantiri et al. 2001) belong to Cylindrocarpon group 1, which have micro- and macroconidia but lack chlamydospores, except N. ramulariae/C. obtusiusculum, which has chlamydospores and lacks microconidia. Cylindrocarpon obtusiusculum was originally placed in Cylindrocarpon group 4 by Booth (1966). The type species of Cylindrocarpon, C. cylindroides, belongs in Cylindrocarpon group 1 (Booth 1966, Mantiri et al. 2001, Brayford et al. 2004, Halleen et al. 2004, Castlebury et al. 2006). Anamorphs in the N. mammoidea/veuillotiana-group (clade II in Mantiri et al. 2001) belong to Cylindrocarpon group 2 and are characterised by the lack of microconidia and chlamydospores. Anamorphs in Cylindrocarpon group 3 belong to the N. radicicola-group (clade III in Mantiri et al. 2001) and are characterised by the presence of microconidia and chlamydospores.

The anamorphic genus Campylocarpon was described by Halleen et al. (2004) for species resembling Cylindrocarpon with 3–5-septate, curved macroconidia and lacking microconidia. Halleen et al. (2004) segregated Campylocarpon from Cylindrocarpon based on molecular phylogenetic data that placed it more closely to N. mammoidea-group than to Cylindrocarpon sensu stricto (N. coccinea-group). Halleen et al. (2004) noted the similarity of Campylocarpon to the Cylindrocarpon anamorphs of species in the N. mammoidea-group.

Even though morphological and phylogenetic studies suggest that Neonectria/Cylindrocarpon represents more than one genus (Samuels & Brayford 1994, Mantiri et al. 2001, Brayford et al. 2004, Halleen et al. 2004, Hirooka et al. 2005, Castlebury et al. 2006, Halleen et al. 2006), formal taxonomic segregation of these groups has not been proposed. The objectives of the present study are to: (1) define Neonectria sensu stricto; (2) determine if Neonectria/Cylindrocarpon should be divided into multiple genera using phylogenetic analyses of multiple loci; and (3) recognise these species in monophyletic genera as a first step toward their taxonomic revision.

MATERIALS AND METHODS

Morphological characterisation

Specimens were obtained from U.S. National Fungus Collections (BPI), Steere Herbarium, New York Botanical Garden (NY), and Manaaki Whenua Landcare Research, New Zealand (PDD), and collected in fresh conidition from the field. Some cultures were obtained from the Centraalbureau voor Schimmelcultures (CBS), Utrecht, Netherlands. For morphological characterisation of the teleomorph, the macromorphology of the perithecia was observed and described using the following characters: distribution of perithecia on the host; perithecial shape, colour, and reaction to 3 % w/v potassium hydroxide (KOH) and 100 % lactic acid; perithecial wall structure; and colour and appearance of the perithecial apex. Colour standards are from Kornerup & Wanscher (1978). To observe internal and microscopic characteristics, the perithecia were rehydrated briefly in KOH, then supported by Tissue-Tek O.C.T. Compound 4583 (Miles Inc., Elkhart, Indiana, USA), and sectioned at a thickness of ca. 15 μm with a freezing microtome. Characteristics of asci and ascospores were observed by rehydrating the perithecia in 3 % KOH, removing part of the centrum with a fine glass needle, and placing it on a glass slide. Microscopic observations were made using an Olympus BX51 microscope and DP71 digital camera. Cultures were obtained by isolating asci containing ascospores on cornmeal-dextrose agar (CMD; Difco™ cornmeal agar + 2 % w/v dextrose supplemented with antibiotics 0.2 % each neomycin and streptomycin). Morphological observations of the colonies and anamorph in culture were based on isolates grown on Difco™ potato-dextrose agar (PDA) and SNA (low nutrient agar, Nirenberg 1976) for 3 wk in an incubator at 25 °C with alternating 12 h/12 h fluorescent light/darkness. Measurements of continuous characters such as length and width for both anamorph and teleomorph were made using the beta 4.0.2 version of Scion Image software (Scion Corporation, Frederick, Maryland, USA). Continuous measurements are based on 10–30 measured units and are reported as the extremes (maximum and minimum) in brackets separated by the mean plus and minus one standard deviation.

DNA extraction, polymerase chain reaction (PCR), and sequencing

Strains listed in Table 1 were grown in Petri dishes (6 cm diam) containing Difco™ potato-dextrose broth. Plates were incubated at 25 °C for ca. 1 wk. DNA was extracted from the mycelial mat harvested from the surface of the broth. The PowerPlant™ DNA Isolation Kit (MO BIO Laboratories, Inc., Carlsbad, California, USA) was used to extract DNA from the samples. Other sequences used in the analyses were obtained from GenBank (Table 1).

Table 1.

Isolates used in the phylogenetic analyses with their corresponding GenBank accession numbers.

Species (sexual/asexual state)^**	Isolate	Isolate
		ITS	LSU	*tef1*	*tub*	*act*	*rpb1*
Campylocarpon fasciculare	CBS 112613		HM364313			HM352881	HM364331
Campylocarpon pseudofasciculare	CBS 112679		HM364314			HM352882	HM364332
Cosmospora coccinea / `Verticillium' olivaceaum	A.R. 2741 (= CBS 114050)	HM484537	AY489734 ^*	HM484515	HM484589	GQ505967 ^*	AY489667 ^*
Cosmospora vilior / Acremonium berkeleyanum	A.R. 4215 (= CBS 126111)	HM484854	HM484869	HM484846	HM484875	HM484838	HM484872
Cosmospora viliuscula	G.J.S. 96-6 (= CBS 455.96)	HM484855	GQ506003 ^*	HM484851	HM484876	GQ505966 ^*	GQ506032 ^*
Cosmospora sp.	G.J.S. 93-15	HM484856	GQ506006 ^*	HM484849	HM484878	GQ505968 ^*	GQ506035 ^*
Cyanonectria cyanostoma / Fusarium sp.	G.J.S. 98-127 (= CBS 101734)	FJ474076 ^*	FJ474081 ^*	HM484611		GQ505961 ^*	GQ506017 ^*
Cylindrocarpon destructans var. crassum (I)	CBS 537.92				EF607079 ^*
Cyl. destructans var. crassum (I)	CBS 605.92	EF607078 ^*			EF607065 ^*
Cyl. olidum (T)	CBS 215.67		HM364317			HM352884	HM364334
Emericellopsis glabra	A.R. 3614 (= CBS 125295)	HM484860	GQ505993 ^*	HM484843	HM484879	GQ505969 ^*	GQ506023 ^*
Gibberella fujikuroi / Fusarium moniliforme	FM 94	FJ755697 ^*
Gibberella fujikuroi / Fusarium moniliforme	PMBMDF092	FJ798606 ^*
Haematonectria haematococca / Fusarium solani	NRRL 22277	AF178401 ^*	AF178370 ^*
Haematonectria illudens / Fusarium illudens	NRRL 22090	AF178393 ^*	AF178362 ^*
Haematonectria sp.	G.J.S. 93-47 (= CBS 125113)	HM484862	HM484870	HM484850	HM484880	HM484839	HM484873
Hydropisphaera fungicola	A.R. 4170 (= CBS 122304)	HM484863	GQ505995 ^*	HM484845	HM484877	GQ505970 ^*	GQ506025 ^*
Lasionectria mantuana	A.R. 4029 (= CBS 114291)	HM484858		HM484844
Leuconectria clusiae / Gliocephalotrichum bulbilium	ATCC 22228		AY489732 ^*				AY489664 ^*
Mycoarachis inversa	A.R. 2745 (= ATCC 22107)	HM484861	GQ505991 ^*	HM484840	HM484882	GQ505972 ^*	GQ506021 ^*
Nectria antarctica	A.R. 2767 (= CBS 115033)	HM484556	HM484560	HM484516	HM484601	HM484501	HM484575
Nectria aquifolii	A.R. 4108 (= CBS 125147)	HM484538	HM484565	HM484522	HM484590
Nectria aurigera	A.R. 3717 (= CBS 109874)	HM484551	HM484573	HM484521	HM484600	HM484511	HM484586
Nectria austroamericana / Gyrostroma austroamericanum	A.R. 2808 (= CBS 126114)	HM484555	GQ505988	HM484520	HM484597
Nectria balansae	G.J.S. 86-117 (= CBS 125119)	HM484857	HM484868	HM484848	HM484874		HM484871
Nectria balsamea	A.R. 4478 (= CBS 125166)	HM484540	HM484567	HM484528	HM484591	HM484508	HM484580
Nectria berolinensis / “Tubercularia” berolinensis	A.R. 2776 (= CBS 126112)	HM484543	HM484568	HM484517	HM484594	HM484510	HM484583
Nectria cinnabarina (dematiosa) / Tubercularia vulgaris	CBS 278.48	HM484682	HM484729	HM484647	HM484800	HM484615	HM484760
Nectria coryli	Y.H. 0815 (= A.R. 4561)	HM484539	HM484566	HM484536	HM484596	HM484509
Nectria cucurbitula / Zythiostroma pinastri	CBS 259.58	HM484541	GQ505998	HM484530	HM484592	GQ505974	GQ506028
Nectria lamyi	A.R. 2779 (= CBS 115034)	HM484544	HM484569	HM484518	HM484593	HM484507	HM484582
Nectria miltina	A.R. 4391 (= CBS 121121)	HM484547			HM484609	HM484514	HM484587
Nectria pseudotrichia / Tubercularia lateritia	CBS 551.84	HM484554		HM484532	HM484602	GQ505976	GQ506030
Nectria pyrrhochlora	A.R. 2786 (= CBS 125131)	HM484545	HM484570	HM484519	HM484598
Nectria sinopica / Zythiostroma mougeotii	CBS 462.83	HM484542	GQ506001	HM484531	HM484595	GQ505973	GQ506031
Nectria zanthoxyli	A.R. 4280 (= CBS 126113)	HM484546	HM484571	HM484523	HM484599	HM484513	HM484585
Nectriopsis exigua / Verticillium rexianum	G.J.S. 98-32 (= CBS 126110)	HM484865	GQ505986 ^*	HM484852	HM484883	GQ505979 ^*	GQ506014 ^*
Neo. castaneicola / Cyl. castaneicola (R)	TPPH 1	AB233175 ^*
Neo. coprosmae / Cyl. coprosmae (I)	G.J.S. 85-39 (= CBS 119606)	HM364301
Neo. coronata / Cyl. coronatum (T)	A.R. 4505 (= CBS 125173)			HM364348	HM352862	HM352878	HM364328
Neo. discophora / Cyl. ianothele (T)	A.R. 4324 (= CBS 125153)	HM364294	HM364307	HM364345	HM352860	HM352875	HM364326
	A.R. 4499 (= CBS 125172)	HM364296	HM364309	HM364347		HM352877	HM364327
Neo. ditissima / Cyl. heteronemum	CBS 100316	HM364298	HM364311	HM364350	HM352864	HM352880	HM364330
Neo. fuckeliana / Cyl. cylindroides var. tenue	A.R. 3103 (= CBS 125133)	HM364291	HM446654	HM364342	HM352857	HM352872
	A.R. 4109 (= CBS 119723)	HM364292	HM364305	HM364343	HM352858	HM352873
	A.R. 4110 (= CBS 119200)	HM364293	HM364306	HM364344	HM352859	HM352874
	A.R. 4480 (= 126652)	HM364295	HM364308	HM364346	HM352861	HM352876
	G.J.S. 02-67 (= CBS 125109)	HM364300	HM364320	HM364354	HM352867	HM352886
Neo. jungneri / Cyl. victoriae (T)	C.T.R. 71-244	HM364299	HM364319	HM364353	HM352866	HM352885	HM364336
Neo. liriodendri / Cyl. liriodendri (I)	CBS 112602	HM364302	HM364323		HM352853
Neo. macrodidyma / Cyl. macrodydimum (I)	CBS 112615		HM364315			HM352883	HM364333
Neo. neobalansae / Cyl. sp. (R)	G.J.S. 85-219 (= CBS 125120)		HM364322		HM352869
Neo. neomacrospora / Cyl. cylindroides var. cylindroides	CBS 198.62		HM364316	HM364351	HM352865
	CBS 324.61		HM364318	HM364352	HM352854		HM364335
Neo. radicicola / Cyl. destructans (I)	A.R. 2553 (= ATCC 208837)	HM364290	HM364304	HM364341	HM352856	HM352871	HM364325
Neo. ramulariae / Cyl. obtusiusculum	ATCC 16237	HM364297	HM364310	HM364349	HM352863	HM352879	HM364329
	CBS 151.29	HM364303	HM364324	HM364340	HM352855
Neo. rugulosa / Cyl. rugulosum (R)	TPPH 32	AB233176 ^*			AB237526 ^*
Neo. trachosa / Cyl. sp. (T)	CBS 112467		HM364312	HM364356			HM364339
Neo. veuillotiana / Cyl. candidulum (T)	G.J.S. 90-48 (= CBS 125118)			HM364357	HM352870	HM352888	HM364338
Neo. westlandica / Cyl. sp. (T)	G.J.S. 83-156 (= CBS 112464)		HM364321	HM364355	HM352868	HM352887	HM364337
Neocosmospora vasinfecta / Acremonium-like	A.R. 3587	HM484864		HM484842	HM484881
Ophionectria trichospora / Antipodium spectabile	G.J.S. 01-206	HM484867		HM484847	HM484886
	CBS 109876		AF543790 ^*				AY489669 ^*
Pseudonectria rousseliana / Volutella buxi	ATCC-MYA 627		U17416 ^*				AY489670 ^*
Rubrinectria olivacea / Nalanthamala sp.	CBS 102268	AY554219 ^*	AY554244 ^*		AY554238 ^*
Selinia pulchra /	A.R. 2812	HM484859	GQ505992 ^*	HM484841	HM484884	GQ505982 ^*	GQ506022 ^*
Verrucostoma freycinetiae / Acremonium-like	MAFF240100/h523	HM484866	GQ506013 ^*	HM484853	HM484885	GQ505984 ^*	GQ506018 ^*
Viridispora diparietispora / Penicillifer furcatus	CBS 114049		AY489735 ^*				AY489668 ^*

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Sequences obtained from GenBank.

^**

Letters in parenthesis represent their classification in the newly segregated genera. I: Ilyonectria; R: Rugonectria; T: Thelonectria.

DNA sequences of partial large subunit (LSU, ca. 900 bp) and complete internal transcribed spacers 1 and 2 (ITS, ca. 600 bp), including 5.8S of the nuclear ribosomal DNA; partial β-tubulin (tub, ca. 500 bp); α-actin (act, ca. 600 bp); RNA polymerase II subunit 1 (rpb1, ca. 700 bp); and translation elongation factor 1α (tef1, ca. 700 bp) were used in the phylogenetic analyses (Table 2). The primers used and PCR protocols are listed in Table 2. Each 25 μL PCR reaction consisted of 12.5 μL Promega GreenTaq™ Master Mix 2× (Promega Corporation, Madison, Wisconsin, USA), 1.25 μL 10 mM forward primer, 1.25 μL 10 mM reverse primer, 1 μL of the DNA template, 1 μL of dimethyl sulfoxide (DMSO), and 8 μL of sterile RNAase-free water. PCR reactions were run in an Eppendorf Mastercycler ep using the parameters detailed in Table 2. PCR products were cleaned with ExoSAP-IT® (USB Corporation, Cleveland, Ohio, USA). Clean PCR products were sequenced at the DNA Sequencing Facility (Center for Agricultural Biotechnology, University of Maryland, College Park, Maryland, USA). Sequences were assembled and edited with Sequencher v. 4.9 (Gene Codes, Madison, Wisconsin, USA). Sequences were deposited in GenBank as listed in Table 1.

Table 2.

Genes/loci used in the phylogenetic analyses. Information on the primers, included bases pairs, PCR protocols, and models of nucleotide substitution are indicated.

Locus	Primers used (reference)	PCR protocol: Annealing temp. & cycles	Nucleotide substitution models	Included sites (# of excluded sites)	Phylogenetically informative sites (%)	Uninformative polymorphic sites	Invariable sites
ITS	ITS5, ITS4 (White et al. 1990)	53 °C, 1 min, 35′	GTR+G	670 (136)	230 (34 %)	95	345
LSU	LR5, LROR (Vilgalys n.d.)	53 °C, 1 min, 35′	TIM+I+G	915 (0)	142 (16 %)	44	729
Tef1	tef1-728, tef1-986 (Carbone & Kohn 1999)	66 °C, 55 s, 9′	GTR+I+G	707 (524)	200 (20 %)	39	468
	tef1-728, tef1-986 (Carbone & Kohn 1999)	56 °C, 55 s, 35′
Tub	Btub-T1, Btub-T2 (O'Donnell & Cigelnik 1997)	55 °C, 30 s, 35′	HKY+I+G	535 (127)	260 (26 %)	49	226
Act	Tact1, Tact2 (Samuels et al. 2006)	65 °C, 30 s, 15′	GTR+I+G	635 (0)	149 (15 %)	37	4498
	Tact1, Tact2 (Samuels et al. 2006)	48 °C, 30 s, 30′
Rpb1	crpb1a, rpb1c (Castlebury et al. 2004)	50 °C, 2 min, 40′	GTR+I+G	722 (52)	378 (52 %)	61	283
Total				4184	1359 (33 %)	325	2500

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Phylogenetic analyses

Sixty-nine strains and their corresponding DNA sequences were analysed. Not all strains had all six loci sequenced and some sequences were obtained from GenBank; see Table 1. Seven species in the Bionectriaceae were selected as the outgroup: Emericellopsis glabra, Hydropisphaera fungicola, Lasionectria mantuana, Mycoarachis inversa, Nectriopsis exigua, Selinia pulchra, and Verrucostoma freycinetiae. The included sequences were aligned with MAFFT v. 5 (Katoh et al. 2005) using the E-INS-i strategy. The alignment was improved by hand with Seaview v. 2.4 (Galtier et al. 1996) and MESQUITE v. 2.5 (Maddison & Maddison 2009). Gaps (insertions/deletions) were treated as missing data. Maximum Likelihood (ML) and Bayesian (BI) analyses were performed with all sequences, first with each gene/locus separately, and then with the combined data sets. A reciprocal 70 % BP threshold (Mason-Gamer & Kellogg 1996, Reeb et al. 2004) was used to determine if partitions could be combined into a single phylogeny.

JMODELTEST (Rannala & Yang 1996, Posada & Buckley 2004, Posada 2008) was used to select the models of nucleotide substitution for the ML and BI analyses. The number of substitution schemes was set to 11, base frequencies +F, rate variation +I and +G, and the base tree for likelihood calculations was set to “ML optimised.” Once the likelihood scores were calculated, the models were selected according to the Akaike Information Criterion (AIC). After jMODELTEST was run, the parameters indicated in Table 2 were used for the ML and BI analyses.

GARLI v. 0.96 (Zwickl 2006) was used for the ML and bootstrap analyses through the Grid computing (Cummings & Huskamp 2005) and The Lattice Project (Bazinet & Cummings 2008), which includes clusters and desktops in one encompassing system (Myers et al. 2008). In GARLI, the starting tree was obtained by stepwise-addition and the number of runs or search replicates was set to 50. Bootstrap (BP) analyses were replicated 2000 times. BI analysis was done with MrBayes v. 3.1.2 (Rannala & Yang 1996, Mau et al. 1999, Huelsenbeck et al. 2001, Huelsenbeck et al. 2002). In MrBayes, data were partitioned by locus and the parameters of the nucleotide substitution models for each partition were set as described in Table 2. Two independent analyses of two parallel runs and four chains were carried out for 10 000 000 generations using MrBayes. Analyses were initiated from a random tree and trees were sampled every 100^th generation. Convergence of the log likelihoods was analysed with TRACER v. 1.4.1 (beast.bio.ed.ac.uk/Tracer). The first 20 % of the resulting trees was eliminated (= “burn in”). A consensus tree (“sumt” option) and posterior probabilities (PP) were calculated in MrBayes. Phycas v. 1.1.2 (www.phycas.org) was used as another tree searching method and also to resolve possible polytomies (“Star Tree Paradox” problem), if any, as proposed by Lewis et al. (2005). Phycas uses reversible-jump MCMC to allow unresolved trees, i.e. with polytomies or very short and poorly supported branches, and fully resolved tree topologies to be sampled during a Bayesian analysis. Unresolved trees generally occur when the time between speciation events is so short or the substitution rate so low that no substitutions occurred along a particular internal edge in the true tree. The number of cycles in Phycas was set to 100 000, sampling every 100 cycles, and with a starting tree obtained randomly.

RESULTS

Molecular phylogenetic analyses

Multiple sequence alignment resulted in 4184 included base pairs, 1 359 (33 %) phylogenetically informative and 2 500 invariable sites; 325 sites presented unique non-informative polymorphic sites (Table 2). Ambiguously aligned regions were excluded from the analyses, especially in ITS, tef1, and tub loci, which possess highly variable regions, i.e. introns (Table 2). Phylogenetic analyses of six loci show high bootstrap (BP) and MrBayes posterior probabilities (PP) for most nodes in the combined cladogram, except for a few of the deeper nodes (Fig. 1). BI PPs were either 100 % (high support) or 50 % (low support). The negative log likelihoods (–Ln) for the ML, BI, and Phycas trees were 44603.27, 44959.23, and 44957.36, respectively. The reversible-jump MCMC run in Phycas resulted in a few improved posterior probabilities for some polytomies or poorly supported nodes in the ML or BI trees (Fig. 1). The reciprocal 70 % BP threshold used to determine topological conflicts between partitions resulted in complete congruence, that is, the topologies of each gene genealogy did not contradict each other (results not shown). This can be evidenced in the high BP and PP support found in most nodes (Fig. 1).

Species with Cylindrocarpon-like anamorphs are contained in two paraphyletic clades (Fig. 1): Clade A with the N. rugulosa-group, N. mammoidea/veuillotiana-groups, and Campylocarpon (72 % BP, 100 % PP) and Clade B with the N. coccinea- and N. radicicola-groups (97 % BP, 100 % PP). These clades correspond generally to those reported by Mantiri et al. (2001). Figure 1 also shows that some of the groups defined by Booth (1959) and Samuels & Brayford (1994), i.e. N. mammoidea-, N. rugulosa-, N. coccinea-, and N. radicicola-groups, are supported by high or moderately high BP and PP values. Campylocarpon, an anamorph genus with morphology similar to Cylindrocarpon especially to those anamorphs in the N. mammoidea-group (Halleen et al. 2004), clusters with the N. mammoidea/veuillotiana-group supported by BI PP (100 %).

The type species of Neonectria, N. ramulariae, and Cylindrocarpon, C. cylindroides, fall in the N. coccinea-group, i.e. Neonectria/Cylindrocarpon sensu stricto (94 % BP, 100 % PP) (Fig. 1), part of Clade B. This group also includes N. ditissima and N. fuckeliana. Morphological characteristics of the Neonectria/Cylindrocarpon sensu stricto clade include perithecia aggregated in an erumpent stroma, perithecial walls generally composed of two regions, somewhat ornamented ascospores, and macroconidia that are generally > 3-septate, cylindrical, and straight (Table 3). This clade is sister to the N. radicicola-group (Clade B). The monophyly of the N. radicicola-group is supported by 98 % BP and 100 % PP (Fig. 1). Characteristics of the teleomorph and anamorph clearly separate the N. radicicola-group from Neonectria/Cylindrocarpon sensu stricto (Table 3). Perithecia in the N. radicicola-group are superficial on the substrate and have a distinctive perithecial wall structure, smooth ascospores, and macroconidia that are straight, < 3-septate, with a prominent basal hilum.

Table 3.

Comparison of major diagnostic morphological characteristics between the newly segregated genera.

Character	Campylocarpon (Clade A)	Rugonectria (Clade A)	Thelonectria (Clade A)	Ilyonectria (Clade B)	Neonectria (Clade B)
Teleomorph groups (Booth 1959, Brayford & Samuels 1993, Samuels & Brayford 1994)	–	N. rugulosa-group	N. mammoidea /veuillotiana-groups	N. radicicola-group	N. coccinea-group
Anamorph groups (Booth 1966)	–		Group 2	Group 3	Groups 1 & 4
Arrangement of perithecia on substrate	Teleomorph unknown	Perithecia, formed on, or sometimes partially immersed within a stroma	Perithecia solitary or in groups, superficial, sometimes seated on an immersed inconspicuous stroma	Generally solitary and loosely attached to substrate	Perithecia clustered on wood, generally seated on an erumpent stroma
Perithecial apex	–	Non-papillate	Most species with a prominent, areolate (darkened) papilla, if not, then at least with a darkly pigmented apex	Broadly conical papilla	Blunt or acute apex, rarely papillate
Perithecial wall	–	Warted, 50–150 μm thick; outer region, including warts, of thick-walled (3–4 μm), globose, 10–20 μm diam; perithecial wall merging with surrounding stroma	Smooth or sometimes warted, 20–50 (–100) μm thick; outer region of intertwined hyphae or cells lacking a definite outline, i.e. textura epidermoidea	Generally smooth to slightly roughened, 35–50 μm thick; outer region of thin-walled, globose, large cells	Generally smooth and shiny, sometimes scurfy, 35–50 μm thick; outer region of small, angular to globose, thick-walled cells (textura epidermoidea in one species)
Ascospores	–	1-septate, striate	Generally 1-septate, smooth, rarely spinulose or striate	1-septate, smooth	1-septate, smooth or finely ornamented
Macroconidia shape	Fusiform, curved, often broadest at upper third, with rounded apical cells and flattened or rounded basal cells, inconspicuous hilum	Fusiform, curved, tapering towards ends (almost Fusarium-like), inconspicuous hilum	Fusiform, curved, often broadest at upper third, with rounded apical cells and flattened or rounded basal cells, inconspicuous hilum	Cylindrical, straight, rounded ends, prominent basal hilum	Cylindrical, generally straight, sometimes slightly curved toward ends, with rounded ends (except in one species, N. fuckeliana, which has fusiform straight conidia with pointed ends); inconspicuous hilum
Macroconidia septation	(1–) 3–5 (–6)-septate, average 4 septa	(3–) 5–7 (–9)-septate	(3–) 5–7 (–9)-septate, average 5	1–3-septate, rarely > 3-septate	3–7 (–9)-septate, average 5-septate
Macroconidia size	(24–) 35–60 (–62) × 6.5–9 μm	(35–) 48–85 × 5–10 μm	(35–) 40–90 (–110) × 4–8 (–11) μm	25–50 (–55) × 5–7.5 μm	35–65 (–110) × 4–7 (–8) μm
Microconidia shape	Absent	Ovoid to cylindrical, hilum inconspicuous	Microconidia rare (seen only on natural substrate)	Ellipsoidal, prominent basal hilum	Ellipsoidal to oblong, inconspicuous hilum
Microconidia size	Absent	(3–) 5–15 (–20) × 2–5 μm	–	3–15 × 2.5–5 (–6) μm	(2–) 6–10 (–15) × (1–) 2–5 (–6) μm
Chlamydospores	Uncommon	Absent	Uncommon (except in T. olida = C. olidum)	Abundant, generally intercalary, single or in chains, becoming brownish	Present in some species
Substrate	Pathogenic on roots and stems of grapevines	On bark of recently killed, dying or diseased trees, often causing cankers	On bark of recently killed, dying or diseased trees, often causing small cankers, sometimes on rotting roots	Generally a root pathogen. Anamorph common in the soil. Perithecia found mostly on decaying herbaceous material, sometimes branches or roots.	Generally on bark, sometimes causing cankers
Geographic distribution	South Africa, Uruguay	Widespread	Widespread, but more common in tropical regions	Widespread	Mostly in temperate regions

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The N. rugulosa group is sister to the N. mammoidea/veuillotiana-group and Campylocarpon (Clade A). The N. rugulosa-group is monophyletic (100 % BP, 100 % PP). It contains species with warted perithecia and a perithecial wall structure generally different that the N. radicicola-group and Neonectria/Cylindrocarpon sensu stricto, striate ascospores, microconidia, and no chlamydospores (Table 3). The clade that includes the N. mammoidea/veuillotiana-group is also supported by high BP and PP values (70 % BP and 100 % PP). Species in this clade have a perithecial wall comprised of thick-walled cells, a knobby or prominent apex, spinulose or tuberculate ascospores, and generally no microconidia or chlamydospores. Campylocarpon sequences form a distinct clade sister to the N. mammoidea-group and is supported by 100 % BP and 100 % PP. No teleomorph is known for Campylocarpon.

Morphological characterisation

Presence or absence of a stroma

In many cases perithecia are solitary, either seated directly on the substratum in the N. radicicola-group or on a minute basal stroma in the N. mammoidea/veuillotiana-group. In other cases, such as in N. discophora and N. lucida, perithecia are seated amidst erect hyphae that arise from a basal, almost inconspicuous stroma. A characteristic of N. coccinea, N. fuckeliana and other species of Neonectria sensu stricto and N. rugulosa-group is that they form in great numbers on a rather extensive, subcortical, basal stroma. An extensive stroma may also form in N. jungneri, but is not as conspicuous as in Neonectria sensu stricto and the N. rugulosa-group.

Perithecial wall

The perithecial wall of species of Neonectria sensu lato comprises at least two regions. The inner region is very thin, consisting of only a few layers of thin-walled, tangentially flattened cells lining the locule where the spores are formed. The outer regions vary. Essentially four distinctive types of outer perithecial walls are found among the groups studied here. In the N. radiciola-group the outer region of the perithecial wall is formed of one or two layers of large, round, thin-walled cells. This anatomy can be discerned even in whole mounts of perithecia. In species that have this anatomy, the surface of the perithecial wall, when seen in face view, is of large, round cells, mirroring what is seen in sections. The second perithecial wall anatomy consists of an outer region that is a palisade of short hyphae that are perpendicular to the locule, e.g. in the N. mammoidea-group. When seen in face view cells at the surface of the perithecium are small, < 5 μm diam. In some species, such as N. trachosa and N. westlandica, a superficial layer of large, angular cells that form warts obscure the palisade. When there is a palisade but no outer layer of large cells, the perithecial surface may be smooth and shiny. This wall anatomy typifies some members of the N. mammoidea-group, e.g. N. discophora, N. lucida, N. westlandica; and N. fuckeliana in Neonectria sensu stricto. The wall of species such as N. coronata or N. jungneri, both in the N. mammoidea/veuillotiana-group, which lacks any apparent cellular structure, is formed of intertwined hyphae having a seemingly random arrangement rather than a palisadal arrangement. The third perithecial wall type is characterised by the formation of thick-walled, round cells in the outer region that can be seen in section and in face view. This wall type characterises species of Neonectria sensu stricto. The fourth type of perithecial wall is that of the N. rugulosa-group. The perithecial wall is thick, 50–150 μm, with the outer region formed of several layers of cells, including warts, with small globose cells that are very thick-walled and merge with the surrounding stroma.

Ascospores

Although some species of Neonectria have been reported to have multiseptate ascospores (Rossman 1983, Samuels & Brayford 1993), the ascospores of the species included in the present study are bicellular. There is a tendency towards having spinulose ornamentation but there are exceptions. In species such as N. veuillotiana the ascospores may be nearly tuberculate. In N. jungneri the spores are coarsely striate. In N. coronata the spinules may be arranged in lines giving the appearance of striations. Ascospores of most species are hyaline, but, in N. discophora, N. lucida, and N. westlandica, the spores become pale yellow-brown. A species not included in the present study, Nectria viridispora, probably in the N. mammoidea-group, has green ascospores. Ascospores of species in Neonectria sensu stricto and the N. radicicola-group are smooth. Species in the N. rugulosa-group have striate ascospores, sometimes inconspicuous; cotton blue may be needed to observe these striations.

Paraphyses

The Nectria-type centrum (Luttrell 1951) is characterised by the formation of “apical paraphyses,” filaments that originate in a meristem situated at the top of the locule. Typically these filaments have dissolved by the time the ascospores form but often chains of saccate cells may persist among maturing asci. Most species of Neonectria sensu stricto have filaments that appear to be free at the apex and thus resemble paraphyses. These paraphyses are septate and constricted at each septum. The paraphyses are abundant especially in N. fuckeliana.

Conidiophores and phialides

Most conidiophores, especially those that give rise to the macroconidia, are formed laterally from hyphae; they are irregularly branched or form fascicles. In the case of Neonectria sensu stricto and the N. rugulosa-group, the macroconidia are produced from irregularly branched conidiophores or fascicles, and the microconidia from simple, generally unbranched, conidiophores. In the case of the N. radicicola-group, macro- and microconidia apparently originate from the same type of conidiophore. These are simple, unbranched or sparsely branched, irregularly or verticillately branched, or rarely densely branched. The N. mammoidea/veuillotiana-group and Campylocarpon produce only macroconidia that originate from irregularly branched conidiophores or fascicles. The morphology of the phialides is highly conserved. Phialides are generally long and cylindrical or somewhat flask-shaped, but mostly long.

Macro- and microconidia

Although the average size of the macroconidia varies among the groups, there is significant overlap. Campylocarpon, the N. radicicola-group, and Neonectria sensu stricto have macroconidia 25–65 × 4–9 μm, smaller than those of the N. mammoidea/veuillotiana- and N. rugulosa-groups that are 40–90 × 4–10 μm. With respect to shape, species in Clade A (Fig. 1) have curved macroconidia and species in Clade B have straight macroconidia. Within Clade A, macroconidia of the N. rugulosa-group can be easily distinguished from those in Campylocarpon and the N. mammoidea/veuillotiana-group. Species in N. rugulosa-group have curved, fusoid macroconidia with tapering ends that are almost Fusarium-like. Campylocarpon and the N. mammoidea/veuillotiana-group also have curved macroconidia but with rounded ends. Even though the macroconidia of Campylocarpon and the N. mammoidea/veuilotiana-group are similar, they can be distinguished on the basis of septation. Campylocarpon has 3–5-septate macroconidia while the N. mammoidea/veuilotiana-group has 5–7-septate macroconidia. Regarding septation of macroconidia, most species have on average five septa, with exceptions. On average species of Campylocarpon have four septa, the N. radicicola-group have up to three septa with exceptions, and N. jungneri (N. mammoidea-group) has generally > 5 septa. Microconidial morphology is highly conserved. They are generally ellipsoidal, 0–1-septate and measure 3–15 × 2–5 μm. Only the N. radicicola-group has microconidia with a prominent hilum or abscission scar. No microconidia are formed in the N. mammoidea/veuillotina-group and Campylocarpon. Some species in Neonectria sensu stricto may produce microconidia, but not as abundantly as in the N. radicicola- and N. rugulosa-groups. The only exception is N. fuckeliana in which microconidia are abundant and macroconidia are infrequently seen.

Chlamydospores

Chlamydospores are formed in the N. radicicola-group, in a few species in Neonectria sensu stricto, and are rarely formed in Campylocarpon. Species in the N. rugulosa-group rarely produce swollen and slightly pigmented hyphae that resemble chlamydospores. Most species in the N. mammoidea/veuillotiana-group do not produce chlamydospores, except in Cylindrocarpon olidum. The chlamydospores of the N. radicicola-group are generally intercalary, single or in chains, and yellow-brown. When produced in Campylocarpon, they are mostly terminal, single, or in chains of 2–3, and also yellow-brown.

Ecology

Species of Neonectria sensu lato and Cylindrocarpon sensu lato are either saprobes or plant pathogens. The only two known Campylocarpon species cause black foot disease of grapevines. Species in the N. mammoidea/veuillotiana-group are only known as saprobes growing on bark of recently killed woody trees. The only exception is Cylindrocarpon olidum, which has been reported as a root pathogen. Members of the N. rugulosa-group and Neonectria sensu stricto also grow on bark of recently killed trees and many species, e.g. N. castaneicola, N. ditissima, N. faginata, N. rugulosa among others, can cause cankers. In contrast, species in the N. radicicola-group are generally found in the soil and cause many root diseases. Based on the present study, the species that are commonly found in the soil causing root rots are the ones that produce chlamydospores. On the other hand, the species that grow on bark do not produce chlamydospores. Members of the N. rugulosa- and N. radicicola-groups are widespread, N. mammoidea/veuillotiana-group are mostly tropical and subtropical, Neonectria sensu stricto occur in temperate regions, and Campylocarpon is known only from South Africa and Uruguay (Abreo et al. 2010).

DISCUSSION

Genus concept

Several morphological characteristics of the teleomorphs and anamorphs have been used in defining informal groups in Nectria sensu lato. In the case of Neonectria and Cylindrocarpon groups (e.g. Booth 1959, 1966), they have been distinguished by the anatomy of the outer regions of the perithecial wall and presence or absence of microconidia and chlamydospores. Results from this study confirm previous suggestions that Neonectria/Cylindrocarpon is paraphyletic, comprising five independent lineages that may be interpreted as distinct genera. These segregate genera usually cannot be distinguished based on a single morphological or ecological character. However, the lineages or segregate genera correlate strongly with a combination of ecology and morphological characters of the perithecia and anamorphs (Table 3). Thus, the following genera are recognised: (1) Neonectria/Cylindrocarpon sensu stricto (N. coccinea-group); (2) N. rugulosa-group, hereafter Rugonectria gen. nov.; (3) N. mammoidea/veuillotiana-group, hereafter Thelonectria gen. nov.; (4) N. radicicola-group, hereafter Ilyonectria gen. nov.; and (5) Campylocarpon. The Neonectria and Cylindrocarpon groups defined by Booth (1959, 1966) based on morphological characters generally agree with the clades observed in the multilocus phylogeny (Fig. 1).

Based on the morphological similarity between Campylocarpon and Thelonectria, it could be argued that these two are congeneric. However, phylogenetic analyses do not support the monophyly of these two genera (short branch length, and low BP and PP supports, Fig. 1). Therefore, Campylocarpon and Thelonectria are recognised as separate. Several morphological and ecological traits aid in distinguishing these two genera (Table 3).

Although Clades A and B (Fig. 1) could be recognised as two genera, the multiple morphological and ecological traits of each of the five segregate genera are distinctive enough to justify their taxonomic subdivision. There are other similar cases in the Ascomycota. For example, although genera with fast-growing Fusarium anamorphs form a monophyletic group, they are still recognised as separate genera and have morphologically different teleomorphs (e.g. Albonectria, Cyanonectria, Gibberella, and Haematonectria) (O'Donnell 1996, Samuels et al. 2009, Luo & Zhuang 2010a). Another example is Calonectria/Cylindrocladium and related genera. Glionectria/Gliocladiopsis, Nectricladiella/Cylindrocladiella, and Xenocalonectria/Xenocylindrocladium all have similar anamorph and teleomorph morphology and were previously classified in Calonectria. Later they were segregated from Calonectria/Cylindrocladium based mostly on anamorph characteristics even though they form a monophyletic group (Rossman 1983, 1993, Schoch et al. 2000, Crous 2002, Samuels et al. 2009, Luo & Zhuang 2010a). A third example is Botryosphaeria sensu lato. Many recognised monophyletic anamorphic genera, e.g. Fusicoccum, Lasiodiplodia, and Neofusicoccum among others, are associated with Botryosphaeria teleomorphs, yet, Botryosphaeria s. l. forms a monophyletic group (Crous et al. 2006).

Results from thepresent study show that Neonectria fuckeliana clusters with Neonectria/Cylindrocarpon sensu stricto, and T. jungneri with Thelonectria. The branch lengths (substitutions/site) that separate these species from Neonectria/Cylindrocarpon sensu stricto and Thelonectria, respectively, are similar to the branch lengths between Thelonectria and Rugonectria (Fig. 1). This could be interpreted as evidence that N. fuckeliana or T. jungneri should be recognised as distinct genera. However, these species are not separated due to the lack of additional morphologically similar species and to avoid monotypic genera with further splitting of genera. It is possible that the addition of morphologically similar species will support the establishment of new genera.

Neonectria/Cylindrocarpon sensu stricto

Neonectria/Cylindrocarpon sensu stricto is characterised by having few to numerous perithecia clustered on wood and seated on an erumpent stroma; perithecial walls are generally composed of two regions with the outer region comprising small, thick-walled cells; generally septate paraphyses; smooth or finely ornamented ascospores; generally straight, typically 5-septate macroconidia with rounded ends; either microconidia or chlamydospores formed, generally not both; and, if microconidia are present, they are produced from simple, generally unbranched, conidiophores and lack a prominent abscission scar. Anamorphs of Neonectria/Cylindrocarpon belong in Booth's groups 1 and 4 (Booth 1966).

Neonectria/Cylindrocarpon sensu stricto species are mostly found in temperate regions on woody substrata, e.g. bark, often causing cankers, and rarely found in soil. This genus includes species such as N. coccinea/C. candidum, N. ditissima/C. heteronemum, N. faginata/C. faginatum, N. fuckeliana/C. cylindroides var. tenue, N. hederae/C. hederae, N. major/Cylindrocarpon sp., N. neomacrospora/C. cylindroides, N. punicea/C. album, and N. ramulariae/C. obtusiusculum (Castlebury et al. 2006). The monophyly of the N. coccinea-group was shown in Castlebury et al. (2006). Although some authors suggested that N. fuckeliana belongs in the N. mammoidea-group based on the morphology of the perithecia (Booth 1959, Brayford et al. 2004), this study supports more recent accounts that place this species close to Neonectria sensu stricto (Halleen et al. 2004, Castlebury et al. 2006, Luo & Zhuang 2010b).

The teleomorph of the type species of Neonectria, N. ramulariae, apparently has not been collected again since it was described by Wollenweber (1917) (Rossman et al. 1999). Rossman et al. (1999) examined the type specimen and noted that it had only immature perithecia along with its anamorph, C. obtusiusculum (= C. magnusianum Wollenw. 1928 non Wollenw. 1926). Domsch et al. (1980) followed Wollenweber (1928) in recognising N. ramulariae to be the teleomorph of C. obtusiusculum (then known as C. magnusianum), based on the anamorph present in the type specimen of N. ramulariae. Although Rossman et al. (1999) designated an iconotype for N. ramulariae, new collections of the anamorph and teleomorph are needed to better describe N. ramulariae/C. obtusiusculum. The morphology of C. obtusiusculum is similar to the anamorphs in Ilyonectria. However, the lignicolous habit, straight macroconidia, absence of microconidia, absence of a prominent basal abscission scar or hilum, and molecular phylogenetic analyses place C. obtusiusculum in Neonectria/Cylindrocarpon sensu stricto.

The segregate genera: Campylocarpon, Ilyonectria, Rugonectria, and Thelonectria

A sister clade to Neonectria/Cylindrocarpon, Ilyonectria (N. radicicola-group), is described here based on Ilyonectria radicicola comb. nov. (anamorph C. destructans). Anamorphs in Ilyonectria belong in Booth's group 3 (Booth 1966). Contrary to Neonectria/Cylindrocarpon, Ilyonectria and its anamorphs are common in the soil and rhizosphere or as agents causing root rots. Chlamydospores are generally present in species of Ilyonectria, possibly as an adaptation for survival in soil. Chlamydospores are generally absent in species that are associated with bark or cankers, e.g. Neonectria, Rugonectria and Thelonectria. Perithecia in Ilyonectria are not as commonly encountered as the anamorphs, and, if found, they are mostly on herbaceous substrata. The species of this genus are cosmopolitan and are found on a wide range of hosts.

Neonectria-like species included here in Ilyonectria are: I. coprosmae/C. coprosmae, I. liriodendri/C. liriodendri, I. macrodydima/C. macrodydimum, and I. radicicola/C. destructans (Samuels & Brayford 1990, Seifert et al. 2003, Halleen et al. 2004, 2006). The monophyly of species in Ilyonectria, viz. the N. radicicola-group, has also been shown in previous studies (Seifert et al. 2003, Halleen et al. 2004, 2006). These studies suggest that C. destructans is a species complex. Thus, defining C. destructans sensu stricto through the examination of many cultures derived from ascospores as well as cultures isolated directly from diverse substrata is a necessary future endeavour. Many other species have been described that may fit in Ilyonectria.

Rugonectria gen. nov. (N. rugulosa-group) is described here based on Rugonectria rugulosa comb. nov. (anamorph C. rugulosum). Members of the genus occur on recently killed or dying woody substrata, mostly bark, and are sometimes found causing cankers. Some species of Neonectria now included in Rugonectria are: R. castaneicola/C. castaneicola, R. neobalansae, and R. rugulosa/C. rugulosum. Another species that may fit in Rugonectria is Nectria pulcherrima (Samuels & Brayford 1994). This species has multiseptate, curved macroconidia with tapering ends, microconidia, and warted perithecia that are caespitose, somewhat immersed in an erumpent stroma, all characteristics of Rugonectria. This species is morphologically similar to R. neobalansae. A new combination has not been made due to the lack of DNA data to confirm its phylogenetic placement.

The new genus Thelonectria is established here to accommodate species in the N. mammoidea- and N. veuillotiana-groups. Species of Thelonectria are mostly tropical and subtropical, and are found on bark of recently killed or dying trees, often causing small cankers, rarely in soil except in one species, C. olidum. Some species included in this genus are: T. coronata/C. coronatum, T. discophora/C. ianothele, T. jungneri/C. victoriae, T. lucida/C. lucidum, T. olida, T. trachosa, T. veuillotiana/C. candidulum, T. viridispora, and T. westlandica (Mantiri et al. 2001, Brayford et al. 2004). Anamorphs in Thelonectria belong in Booth's group 2 (Booth 1966).

Although Thelonectria can generally be recognised by perithecia with prominent or darkened papilla, macroconidia that are curved with rounded ends, > 3-septate (average 5-septate), and absence of microconidia, some species deviate from this trend. For example, 3-septate macroconidia have been reported for T. lucida and T. trachosa (Booth 1966, Brayford et al. 2004). Thelonectria trachosa mostly forms 3-septate macroconidia, but > 3-septate macroconidia can be found in the same culture (Brayford et al. 2004). Brayford et al. (2004) reported that the majority of the T. lucida cultures formed > 3-septate macroconidia. Brayford et al. (2004) also suggested that T. lucida might comprise a species complex, thus, further taxonomic studies are needed to explain the morphological variation within this species. Thelonectria lucida and T. trachosa can be easily classified in Thelonectria based on the anatomy of the perithecia and curved macroconidia with rounded ends and absence of microconidia and chlamydospores. A similar case is T. olida, which produces 3–5-septate macroconidia and chlamydospores although Booth (1966) reports many > 3-septate macroconidia. This species is classified in Thelonectria based on the curved macroconidia with rounded ends and absence of microconidia. However, T. olida is difficult to distinguish from Campylocarpon based on morphology and ecology.

Conidia in Campylocarpon are similar to those in Thelonectria, as also reported by Halleen et al. (2004). The only morphological difference is the average number of septa in the macroconidia: four in Campylocarpon and five in Thelonectria. Despite the morphological similarity of the conidia, phylogenetic analysis distinguishes the two genera. Campylocarpon species were collected from diseased roots and stems of grapevines in South Africa. This is in contrast to most species of Thelonectria, which are found on above ground parts of woody plants. Thelonectria olida, associated with roots, is the exception.

Previous molecular phylogenetic studies (Mantiri et al. 2001, Brayford et al. 2004) did not show that the N. rugulosa-group was distinct from N. mammoidea-group, as suggested by Samuels & Brayford (1994). This was probably due to the few phylogenetically informative loci and few taxa that were used in those studies. The monophyly of the N. rugulosa-group (= Rugonectria) and its close relationship to the N. mammoidea/veuillotiana-group (= Thelonectria) are shown here (Fig. 1). Rugonectria is distinguished from Thelonectria by perithecial anatomy, presence of microconidia in Rugonectria, and morphology of the macroconidia (Table 3).

As has been the case with several groups of fungi (Chaverri et al. 2003, Frisvad & Samson 2004, Schmidt et al. 2004, Samuels et al. 2006a, Chaverri et al. 2008, Degenkolb et al. 2008, Andersen et al. 2009), a multiphasic approach, i.e. using a combination of independently derived characters such as morphological, ecological, and molecular phylogenetic, is necessary to identify monophyletic groups with Neonectria/Cylindrocarpon-like morphology. For example, the presence of microconidia alone is not useful to identify groups with Cylindrocarpon-like morphology, because microconidia are always present in Ilyonectria and Rugonectria, sometimes present in Neonectria, and absent in Thelonectria, and their morphology is highly conserved. However, if characters are combined such as the presence of 3-septate, straight macroconidia with a prominent abscission scar, presence of chlamydospores, and perithecia with a particular wall anatomy, they can be used to classify a particular specimen as Ilyonectria. Thus, in this study our genus concept is based on a multilocus phylogenetic analyses correlated with a combination of multiple morphological and ecological characters. Each of the proposed genera is further described in the Taxonomy section.

Species of Neonectria/Cylindrocarpon of uncertain classification

In this study we present a general overview of genera with Neonectria/Cylindrocarpon-like morphology. There are still species classified in Neonectria and Cylindrocarpon that have teleomorph and anamorph morphology different than those presented here and also quite distinct from Neonectria/Cylindrocarpon sensu stricto. Additional specimens, cultures, and DNA sequences are needed to infer their phylogenetic position within the Nectriaceae. For example, Neonectria macroconidialis has morphological characteristics of both Neonectria sensu stricto and Ilyonectria. This species is not formally included in Ilyonectria because phylogenetic studies including this species in the ITS tree (Seifert et al. 2003, Halleen et al. 2004) show low bootstrap support for the clade with N. macroconidialis and other species in the N. radicicola-group. In contrast, the β-tubulin tree places N. macroconidialis basal and outside the N. radicicola complex. Therefore, the phylogenetic position of this species is uncertain. The straight macroconidia, prominent basal hilum, and anatomy of the perithecia suggest that N. macroconidialis belongs in Ilyonectria. However, the > 4-septate macroconidia, a characteristic of Neonectria sensu stricto, would be an exception if this species were included in Ilyonectria. This species and others previously placed in the N. radicicola-group (Samuels & Brayford 1990) are morphologically atypical of this group, specifically N. austroradicicola/C. austrodestructans and N. radicicola variant ex Gahnia.

Brayford & Samuels (1993) described three species of Nectria with Cylindrocarpon-like anamorphs and mentioned that they could not be classified in any of the then recognised groups of Nectria. Nectria neblinensis and N. verrucospora are distinct because they have macroconidia that are torpedo-like, viz. straight, wider near the middle or towards the base, and tapering and truncated at the ends. The perithecial wall anatomy somewhat resembles Rugonectria, but the ascospores in these two species are warted and not striate as in Rugonectria. Other species that have been placed in Cylindrocarpon that have torpedo-like macroconidia are C. fusiforme, C. supersimplex, and N. laetidiscoides; however, these are straight in the middle and the terminal cells taper almost to a point (Matsushima 1975, Samuels & Brayford 1993).

Several species previously classified in Neonectria/Cylindrocarpon are distinct from those treated here because they have phragmosporous ascospores, e.g. N. fusispora, N. laetidisca, N. laetidiscoides, N. phaeodisca, N. philodendri, N. septospora and N. vermispora among others (Rossman 1983, Samuels & Brayford 1993). Most of the above appear to belong in Thelonectria, or at least they are closely related, except N. laetidiscoides and N. septospora, which have distinct macroconidia.

Another species with uncertain affinity is N. cinnamomea. The perithecia do not change colour in 3 % KOH, a typical characteristic of members of the Nectriaceae (Brayford & Samuels 1993). In addition, the perithecial wall is completely different from the genera treated in this study or any other genus in Nectriaceae, and the ascospores have a conspicuous wrinkled sheath. The macroconidia are also distinct; they are curved, fusiform, and 3-septate.

Luo & Zhuang (2010b) described Neonectria shennongjiana based mostly on the distinctive macroconidia that are cylindrical-clavate to clove-shaped. The phylogenetic analysis in Luo & Zhuang (2010b) shows that N. shennongjiana may be closely related to Neonectria sensu stricto. Their parsimony cladogram reveals that N. shennongjiana clusters within Neonectria sensu stricto (BP 72 % if N. fuckeliana is included). However, in their phylogenetic tree based on parsimony analysis of two loci (ITS nrDNA and tub), the position of N. shennongjiana is not clear. The bootstrap value supporting the clade of N. shennongjiana and C. obtusisporum is low (62 %). Additional phylogenetic and taxonomic studies are needed to confirm if N. shennongjiana and other species with odd-shaped macroconidia belong in Neonectria s.str. Another species with clove-shaped macroconidia is described in the literature, i.e. Nectria lugdunensis (Webster 1959), the teleomorph of Heliscus lugdunensis.

TAXONOMY

Many of the species of Neonectria sensu lato, including those considered here, are known in both their teleomorph and anamorph states. Although Article 59 of the International Code of Botanical Nomenclature (ICBN) allows the use of two scientific names for some groups of pleomorphic fungi including ascomycetes, a trend exists toward the use of just one scientific name for each species regardless of the state manifested (Rossman & Samuels 2005, Rossman 2009). Additionally, generic names of asexual fungi are now being used in a narrower, phylogenetic sense rather than as broad form-genera that encompass unrelated fungi. For example, the genus Verticillium sensu lato, which traditionally included many species with verticillate branching, has been segregated into distinct phylogenetic genera in spite of morphological similarities. Recently, Verticillium sensu stricto was conserved with a different type so that it represents the plant pathogenic species such as V. alboatrum and V. dahliae (Zare et al. 2004). Moreover, other genera separated from Verticillium sensu stricto are now recognised based on distinctive morphological and ecological characteristics, e.g. Lecanicillium and Pochonia (Gams & Van Zaayen 1982, Zare et al. 2000, Gams & Zare 2001, Zare & Gams 2001a, b, Zare et al. 2001).

The anamorphs of Neonectria sensu lato have been classified in the genus Cylindrocarpon. Just as Neonectria is now conceived in a narrow sense, the genus Cylindrocarpon is herein defined phylogenetically and restricted to only anamorphs of Neonectria sensu stricto. Thus, the anamorph name in Cylindrocarpon is listed for only those species that belong in Neonectria sensu stricto. However, for species in genera segregated from Neonectria sensu lato with an anamorph name in Cylindrocarpon, the scientific name of the anamorph is listed in quotes, e.g. “Cylindrocarpon” destructans, or as Cylindrocarpon-like, if no epithet exists, to indicate that it does not belong in Cylindrocarpon sensu stricto.

In this paper, some species described in Cylindrocarpon have no known teleomorph, but, phylogenetically, they fall into a recognised genus (e.g. “C.” olidum = Thelonectria olida comb. nov). As permitted by the ICBN this scientific name is recombined in the new genus. Recent examples in the literature include Lombard et al. (2009), in which species are described in Calonectria despite the lack of known teleomorphs. Although it would be possible and correct according to ICBN Art. 59 to place these taxa into newly described or existing anamorph genera, this has not been done to avoid separating anamorph names from holomorph genera, which is redundant, confusing, and unnecessary. If and when a teleomorph were discovered for this species and a new name were proposed for it, at present, priority would be given to that teleomorph name rather than the anamorph name. Alternatively, the anamorph name could be epitypified with an element that represents the teleomorph in accordance with ICBN Art. 59.7. Given the confusion that has arisen because of the dual nomenclature associated with pleomorphic fungi and the usefulness of molecular systematics in determining the accurate taxonomic placement of asexually reproducing fungi, it would seem expedient to move toward the use of only one scientific name for all fungi.

KEY TO SEGREGATE GENERA OF NEONECTRIA/CYLINDROCARPON

1. Perithecia generally on herbaceous material, rarely on bark or woody parts; perithecia superficial, loosely attached to substratum; perithecial wall of two regions, outer region of thin-walled (ca. 1 μm), globose, large cells; ascospores smooth; anamorph in soil, generally associated with diseased roots; microconidia generally with a prominent abscission scar; chlamydospores present; macroconidia straight, generally < 3-septate, generally with a prominent abscission scar............................................................................................ Ilyonectria 1. Perithecia and macroconidia not as above............................................................................................................................................... 2
2. Perithecia smooth to slightly roughened, generally red, with a prominent papilla or non-papillate; ascospores generally smooth or slightly ornamented; microconidia present or absent; chlamydospores present or absent; macroconidia curved or almost straight, with rounded ends, generally 3–5-septate; on bark or roots........................................................................................................................................... 3 2. Perithecia conspicuously warted, orange-red, generally aggregated, with an inconspicuous papilla, perithecial wall 50–150 μm thick; ascospores striate; microconidia present; chlamydospores absent; macroconidia fusiform with tapering ends; generally on bark of recently killed trees or causing small cankers......................................................................................................................... Rugonectria
3. Perithecia clustered on wood, generally seated on an erumpent stroma, generally smooth and shiny, sometimes scurfy with a blunt or acute apex, rarely papillate; perithecial walls of 2–3 regions, outer region of small, angular to globose, thick-walled cells, rarely of textura epidermoidea; many species with septate paraphyses; ascospores ellipsoidal, smooth or finely ornamented; either microconidia or chlamydospores present; macroconidia generally straight or slightly curved toward ends, rarely clove-shaped, with rounded ends, rarely tapering, 5–7-septate; chlamydospores rare; on bark of recently killed trees or forming cankers.................................. Neonectria 3. Perithecia mostly aggregated, generally smooth and shiny, with a prominent papilla; ascospores generally ornamented; microconidia and chlamydospores absent; macroconidia curved, often broadest at upper third, with rounded apical cells and flattened or rounded basal cells, 3–7-septate; on bark of recently killed trees, on small cankers, or diseased roots.......................................................................... 4
4. Teleomorph unknown; macroconidia on average 4-septate; on diseased roots and stems of grapevines; generally pathogenic; macroconidia generally 3–5-septate (average 4); known from South Africa and Uruguay................................................ Campylocarpon 4. Teleomorph common, on bark of recently killed trees or causing small cankers; perithecia superficial, most species with a prominent, darkened papilla, if not, then at least with a darkly pigmented apex; perithecial walls of 2–3 regions; outer region of intertwined hyphae or cells lacking a definite outline i.e. textura epidermoidea, with thickened and pigmented walls; ascospores mostly ornamented, becoming brownish at maturity; anamorphs rarely encountered apart from their teleomorph; macroconidia (4–)5–7(–9)-septate (average 5) (except T. olida; see section on Description of Genera)....................................................................................................................... Thelonectria

DESCRIPTION OF GENERA

In this paper five genera are described that have neonectria- and Cylindrocarpon-like morphology: Campylocarpon (teleomorph unknown); Ilyonectria gen. nov. (anam. Cylindrocarpon-like); Neonectria sensu stricto (anam. Cylindrocarpon sensu stricto); Rugonectria gen. nov. (anam. Cylindrocarpon-like); and Thelonectria (anam. Cylindrocarpon-like). New combinations are made only for those species that are confirmed to belong to the new genera based on molecular phylogenetic data presented here or in previous studies (Seifert et al. 2003, Brayford et al. 2004, Halleen et al. 2004, 2006, Castlebury et al. 2006).

CAMPYLOCARPON Halleen, Schroers & Crous, Stud. Mycol. 50: 449. 2004. Fig. 2.

Type: Campylocarpon fasciculare Schroers, Halleen & Crous, Stud. Mycol. 50: 449. 2004.

Teleomorph: Unknown.

Anamorph: Cylindrocarpon-like; microconidia not observed; chlamydospores rarely observed; conidiophores arising laterally from hyphae, irregularly branched conidiophores or forming fascicles; phialides cylindrical, (13–)15–20(–25) × (2–)3.5–4 μm; macroconidia curved, often broadest at upper third, with rounded apical cells and flattened or rounded basal cells, (1–)3–5(–6)-septate (average 4), with inconspicuous hilum, (24–)35–60(–62) × 6.5–9 μm.

Habitat: On roots and stems of grapevines; generally pathogenic.

Distribution: Known from South Africa and Uruguay (Abreo et al. 2010).

Campylocarpon fasciculare Schroers, Halleen & Crous, Stud. Mycol. 50: 449. 2004.

Teleomorph: Unknown.

Habitat: On diseased roots, rootstock and stems of grapevines.

Distribution: South Africa.

Description and illustrations: Halleen et al. (2004).

Campylocarpon pseudofasciculare Halleen, Schroers & Crous, Stud. Mycol. 50: 451. 2004.

Teleomorph: Unknown.

Habitat: On asymptomatic grapevine roots.

Distribution: South Africa.

Description and illustrations: Halleen et al. (2004).

ILYONECTRIA P. Chaverri & C. Salgado, gen. nov. MycoBank MB518558. Fig. 3.

Type: Ilyonectria radicicola (Gerlach & L. Nilsson) Chaverri & C. Salgado.

Etymology: “ilyo” = Greek for “mud” or “dirt”. The name is given because most species are found as soil inhabitants.

Ascomata superficialia, globosa vel sublobosa, verrucata vel squamosa, rubra, KOH+ phaeorubra, papilla conica vel subconica. Ascosporae ellipsoidea, 1-septatae, hyalinae, glabra. Anamorphosis cylindrocarpon-similis. Microconidia et chlamydosporae abundans. Phialide cylindrici. Macroconidia cylindrici, recte, hyaline, 1–3-septatae, hilum conspicue. Microconidia ellipsoidea vel oblonga, hyaline, 0–1-septatae, hilum conspicue. Typus: Ilyonectria radicicola.

Teleomorph: Perithecia superficial, loosely attached to substrate, red, KOH+, globose to subglobose, 175–350 μm diam, with a broadly conical papilla, scaly or slightly warted; perithecial wall of two regions, 35–50 μm thick: outer region 25–30 μm thick, of thin-walled, ca. 1 μm, globose, large cells; inner region of compressed, flattened cells. Ascospores ellipsoidal, 1-septate, smooth, hyaline.

Anamorph: Cylindrocarpon-like; microconidia and chlamydospores abundant; macro- and microconidia apparently originating from same conidiophores. Conidiophores 40–160 um long, generally simple, unbranched or sparsely branched, irregularly or verticillately branched, rarely densely branched. Phialides cylindrical, 15–40 (–50) × 1.5–3 μm. Macroconidia straight, hyaline, 1–3-septate, rarely > 3-septate, 25–50(–55) × 5–7.5 μm, generally with a prominent basal or lateral abscission scar or hilum. Microconidia ellipsoidal to ovoid, hyaline, 0–1-septate, with a lateral or basal hilum, 3–15 × 2.5–5(–6) μm. Chlamydospores abundant, generally intercalary, globose, single or in chains, becoming brownish.

Habitat: On roots, soil, woody and herbaceous plants, often pathogenic.

Notes: One potential existing generic name for this group is Coleomyces Moreau & M. Moreau that Booth (1966) listed as a synonym of Cylindrocarpon. The illustration in the original description of Coleomyces, based on C. rufus (Moreau & Moreau 1937), suggests that it belongs in the N. radicicola-group. However, in the original description the authors refer to this name as “ad interim.” Ad interim means it is a provisional name and, according to the ICBN (Art. 34.1, Ex. 6), it is not validly published. The authors of the present study were not able to find a later publication validating this name. Therefore, Coleomyces cannot be used for species in the N. radicicola-group.

Ilyonectria coprosmae (Dingley) P. Chaverri & C. Salgado, comb. nov. MycoBank MB518559. Basionym: Nectria coprosmae Dingley, Trans. Roy. Soc. New Zealand 79: 200. 1951.

≡ Nectria radicicola var. coprosmae (Dingley) Samuels & Brayford, Mycol. Res. 94: 438. 1990.
≡ Neonectria coprosmae (Dingley) Seifert, Phytopathology 93: 1541. 2003.

Anamorph: “Cylindrocarpon” coprosmae C. Booth, Mycol. Pap. 104: 16. 1966. Basionym: Cylindrocarpon destructans var. coprosmae (C. Booth) Brayford & Samuels, Mycol. Res. 94: 438. 1990.

Habitat: On various decaying woody and herbaceous plants.

Distribution: New Zealand.

Descriptions and illustrations: Booth (1966) and Samuels & Brayford (1990).

Notes: Brayford & Samuels (1990) accepted this species as a variety of Cylindrocarpon destructans. However, Seifert et al. (2003) recognised it as a separate species. To better elucidate the taxonomic and phylogenetic relationship of I. coprosmae/'C.' coprosmae to I. radicicola/'C.' destructans sensu stricto, further detailed taxonomic studies are needed.

Ilyonectria radicicola (Gerlach & L. Nilsson) P. Chaverri & C. Salgado, comb. nov. MycoBank MB518560. Basionym: Nectria radicicola Gerlach & L. Nilsson, Phytopath. Z. 48: 225. 1963.

≡ Neonectria radicicola (Gerlach & L. Nilsson) Mantiri & Samuels, Canad. J. Bot. 79: 339. 2001.

Anamorph: “Cylindrocarpon” destructans (Zinssm.) Scholten var. destructans, Netherl. J. Plant Path. 70 suppl. (2): 9. 1964. Basionym: Ramularia destructans Zinssm., Phytopathology 8: 570. 1918.

= Cylindrocarpon radicicola Wollenw., Fus. Autogr. Delin. 2: 651. 1924.
[= Ramularia macrospora Wollenw. Phytopathology 3: 222. 1913 non Fresen., Beitr. Mykol. 3: 88. 1863. hom. illeg.]
[= Fusarium polymorphum Marchal, Bull. Soc. Roy. Bot. Belgique 34: 145-148. 1895 non Matruchot, Rech. Dével. Mucéd. 84: 1892. hom. illeg.]

Habitat: On soil, roots, wood, and herbaceous debris.

Distribution: Cosmopolitan.

Descriptions and illustrations: Booth (1966, 1967), Samuels & Brayford (1990).

Ilyonectria liriodendri (Halleen et al.) P. Chaverri & C. Salgado, comb. nov. MycoBank MB518561. Basionym: Neonectria liriodendri Halleen, Rego & Crous, Stud. Mycol. 55: 232. 2006.

Anamorph: “Cylindrocarpon” liriodendri J.D. MacDon. & E.E. Butler, Pl. Dis. 65: 156. 1981.

Habitat: On diseased roots and rootstocks.

Distribution: France, Portugal, New Zealand, South Africa, USA.

Description and illustrations: Halleen et al. (2006).

Ilyonectria macrodidyma (Halleen, Schroers & Crous) P. Chaverri & C. Salgado, comb. nov. MycoBank MB518562. Basionym: Neonectria macrodidyma Halleen, Schroers & Crous, Stud. Mycol. 50: 446. 2004.

Anamorph: “Cylindrocarpon” macrodidymum Schroers, Halleen & Crous, Stud. Mycol. 50: 447. 2004.

Habitat: On diseased roots and rootstocks.

Distribution: Australia, Canada, New Zealand, South Africa.

Description and illustrations: Halleen et al. (2004).

NEONECTRIA Wollenw., Ann. Mycol. 15: 52. 1917. Fig. 4.

Type: Neonectria ramulariae Wollenw.

= Chitinonectria Morelet, Bull. Soc. Sci. Nat. Archéol. Toulon Var 178: 6. 1969. Type: Ch. coccinea (Pers.: Fr.) Morelet (≡ Sphaeria coccinea Pers.: Fr., ≡ Neonectria coccinea (Pers.: Fr.) Rossman & Samuels).

Anamorph: Cylindrocarpon Wollenw., Phytopathology 3: 225. 1913. Type species Cylindrocarpon cylindroides Wollenw.

[= Fusidium Link: Fr., Syst. Mycol. 1: x1. 1821: 3(2): 480. 1832 nomen rejiciendum]

Teleomorph: Perithecia clustered on wood, generally seated on an erumpent stroma, red, KOH+ dark red, yellow in lactic acid, generally smooth and shiny, sometimes scurfy, subglobose to broadly obpyriform, 200–400 μm diam, generally not collapsing when dry, with a blunt or acute apex, rarely papillate. Perithecial walls of 2–3 regions, generally 35–50 μm thick: outer region of small, angular to globose, thick-walled cells, rarely of textura epidermoidea; inner region of flattened thin-walled cells. Paraphyses when present, septate, slightly constricted at each septum. Ascospores ellipsoidal, smooth or finely ornamented, 1-septate, hyaline, sometimes becoming pale brown at maturity.

Anamorph: Either microconidia or chlamydospores present. Macroconidia produced from irregularly branched conidiophores or fascicles. Phialides cylindrical, typically 10–20(–30) μm long. Macroconidia hyaline, smooth, generally straight, sometimes slightly curved toward ends, with rounded ends except in one species, N. fuckeliana, which has fusiform conidia with pointed ends, 3–7(–9)-septate, mostly 5-septate, lacking a prominent scar or basal hilum, 35–65(–110) × 4–7(–8) μm. Microconidia produced from simple, generally unbranched conidiophores, short or long; microconidia hyaline, smooth, ellipsoidal to oblong, 0–1-septate, mostly unicellular, (2–)6–10(–15) × (1–)2–5(–6) μm. When present, chlamydospores globose to subglobose, hyaline.

Habitat: Generally on bark, sometimes causing cankers. Mostly in temperate regions.

Representative species: N. coccinea/C. candidum, N. ditissima/C. heteronemum, N. faginata/C. faginatum, N. fuckeliana/C. cylindroides var. tenue, N. hederae/C. hederae, N. major/Cylindrocarpon sp., N. neomacrospora/C. cylindroides, N. punicea/C. album, and N. ramulariae/C. obtusiusculum.

Notes: Three names exist that could be considered synonyms of Cylindrocarpon, i.e. Allantospora, Cylindrodendrum, and Heliscus (Booth, 1966). The protologue and illustrations of Allantospora suggest that it is probably not congeneric with Cylindrocarpon (Wakker 1895). Wakker (1895) illustrated this genus based on the type species, A. radicicola, as having Verticillium-like conidiophores and small, allantoid conidia. Therefore, this synonymy is doubtful. Cylindrodendrum could also be considered a synonym of Cylindrocarpon, based on the cylindrical conidia although many other genera in the Hypocreales have cylindrical conidia. The original description and illustration of this genus based on the type species, Cylindrodendrum album, shows that most characteristics are quite distinct from Cylindrocarpon (Bonorden 1851). In Cylindrodendrum, the conidiophore branches have sterile, terminal elongations that are generally hooked, phialides with a swollen base and narrow neck, and relatively small conidia that are not septate. Regarding Heliscus as a possible synonym of Cylindrocarpon, Luo & Zhuang (2010b) placed the morphologically similar species, Neonectria shennongjiana, close to Neonectria/Cylindrocarpon sensu stricto. However, more studies are needed to confirm if Heliscus is congeneric with Cylindrocarpon. If it is congeneric, then, Heliscus is an older name (1880) and thus would have priority over Cylindrocarpon. Due to the extensive use of the name Cylindrocarpon, its economic importance, and some doubts about the phylogenetic placement of Heliscus, the authors of the present study would argue for conservation of Cylindrocarpon over Heliscus.

RUGONECTRIA P. Chaverri & Samuels, gen. nov. MycoBank MB518563. Fig. 5.

Etymology: “rugo” = Latin for “wrinkled”. The perithecial wall surface for species of this genus is warted or rugose.

Type: Rugonectria rugulosa(Pat. & Gaill.) Chaverri, C. Salgado & Samuels.

Ascomata superficialia vel gregaria in stromatae, ascomata globosa vel sublobosa, verrucata vel tuberculata, rubra, KOH+ phaeorubra, non papillata. Ascosporae ellipsoidea vel oblongata, 1-septatae, hyalinae vel pallide brunneae, striatae. Anamorphosis Cylindrocarpon-similis. Phialide cylindrici. Macroconidia fusiformes, hyaline, (3–)5–7(–9)-septatae, hilum inconspicue. Microconidia ellipsoidea vel cylindrici, hyaline, 0–1-septatae, hilum inconspicue. Chlamydosporae absens. Typus: R. rugulosa.

Teleomorph: Perithecia solitary or in groups, formed on or sometimes partially immersed within a stroma. Perithecia globose to subglobose, warted, non-papillate, orange to red, dark red in KOH+, yellow in lactic acid. Perithecial wall 50–150 μm thick, generally of two indistinct regions: outer region including warts with cells circular, 10–20 μm diam, cell walls 3–4 μm thick, merging with surrounding stroma; inner region with cells becoming progressively flattened, thinner, and less pigmented toward locule. Ascospores ellipsoidal to oblong, striate, hyaline, or sometimes yellowish, bicellular.

Anamorph: Cylindrocarpon-like; microconidia present; chlamydospores lacking. Macroconidia arising laterally from hyphae, irregularly branched conidiophores or in fascicles, generally with a short base. Phialides from macroconidiophores cylindrical, 15–25 × 3–5 μm. Macroconidia curved, fusiform, tapering towards ends, (3–)5–7(–9)-septate, with inconspicuous hilum, (35–)48–85 × 5–10 μm. Microconidia produced from simple monophialidic or sparsely branched conidiophores, scattered, ca. 20–100 μm long. Phialides from microconidiophores cylindrical, 20–40 × 3–4 μm. Microconidia ovoid to cylindrical, with rounded ends, generally blunt, 0–1-septate, hyaline, (3–)5–15(–20) × 2–5 μm, lacking a prominent basal hilum.

Habitat: On bark of recently killed, dying or diseased trees, often causing cankers.

Rugonectria castaneicola(W. Yamam. & Oyasu) Hirooka & P. Chaverri, comb. nov. MycoBank MB518564. Basionym: Nectria castaneicola W. Yamam. & Oyasu, Sci. Rep. Hyogo Univ. Agric. Biol. 3: 17. 1957.

≡ Neonectria castaneicola (W. Yamam. & Oyasu) Tak. Kobay. & Hirooka, J. Gen. Plant Pathol. 71: 126. 2005.

Anamorph: “Cylindrocarpon” castaneicola Tak. Kobay. & Hirooka, J. Gen. Plant Pathol. 71: 126. 2005.

Habitat: On bark of conifers, generally causing cankers.

Distribution: Japan.

Description and illustrations: Kobayashi et al. (2005).

Rugonectria neobalansae (Samuels) P. Chaverri & Samuels, comb. nov. MycoBank MB518565. Basionym: Nectria neobalansae Samuels, Mem. N. Y. Bot. Gard. 59: 60. 1990.

Anamorph: Cylindrocarpon-like.

Habitat: On bark of living and recently killed trees.

Distribution: Indonesia, known only from the type locality.

Description and illustrations: Samuels et al. (1990).

Notes: Rugonectria neobalansae is distinct in being almost completely immersed in an orange-red stroma and having, large striate ascospores.

Rugonectria rugulosa (Pat. & Gaill.) Samuels, P. Chaverri & C. Salgado, comb. nov. MycoBank MB518566. Basionym: Nectria rugulosa Pat. & Gaill., Bull. Soc. Mycol. France 4: 115. 1888 [1889].

≡ Neonectria rugulosa (Pat. & Gaill.) Mantiri & Samuels, Canad. J. Bot. 79: 339. 2001.
= Nectria congoensis Sydow in Hennings in Wildeman Mycetes. Ann. Mus. Congo. Bot. V. Études Syst. Geog. Bot. Flore du Baset du Moyen Congo 14. 1909.

Anamorph: “Cylindrocarpon” rugulosum Brayford & Samuels, Sydowia 46: 146. 1994.

Habitat: On bark of living and recently killed trees, sometimes causing cankers.

Distribution: Pantropical.

Descriptions and illustrations: Samuels et al. (1990), Samuels & Brayford (1994).

THELONECTRIA P. Chaverri & C. Salgado, gen. nov. MycoBank MB518567. Fig. 6.

Etymology: “thelo” –Greek for “nipple”. Many species in this genus have a raised, papilla that is sometimes darkened, and thus resembles a nipple.

Type species: Thelonectria discophora (Mont.) P. Chaverri & C. Salgado (new combination made below).

Ascomata superficialia vel gregaria, ascomata globosa vel sublobosa, glabra, rubra, KOH+ phaeorubra, atropapillata. Ascosporae ellipsoidea vel oblongata, 1-septatae, hyalinae, glabra. Anamorphosis Cylindrocarpon-similis. Phialide cylindrici. Macroconidia fusiformes, curva, saepe triente apicali latiore, cellulis apicalibus rotundatis et cellulis basalibus rotundatis vel complanatis, hyaline, (3–)5–7(–9)-septatae, hilum inconspicue. Microconidia absens. Chlamydosporae absens. Typus: T. discophora.

Teleomorph: Perithecia superficial, sometimes seated on an immersed inconspicuous stroma, smooth or sometimes warted, sometimes shiny, globose, subglobose, or pyriform to elongated, 300–600 μm diam, most species with a prominent, areolate (darkened) papilla, if not, then at least with a darkly pigmented apex; perithecial walls of 2 or 3 regions, 20–50(–100) μm thick: outer region of intertwined hyphae or cells lacking a definite outline i.e. textura epidermoidea, with thickened, pigmented walls; inner region of thin-walled, non-pigmented, flattened cells. Ascospores mostly smooth, rarely spinulose or striate, hyaline, becoming brownish at maturity, generally 1-septate.

Anamorph: Cylindrocarpon-like; microconidia rare, sometimes seen on natural substrata; chlamydospores rare, abundant in one species; conidiophores arising laterally from hyphae, irregularly branched conidiophores or forming fascicles; phialides cylindrical, 10–25 × 3–6 μm; macroconidia curved, often broadest at upper third, with rounded apical cells and flattened or rounded basal cells, (3–)5–7(–9)-septate, with inconspicuous hilum, (35–)40–90(–110) × 4–8(–11) μm.

Habitat: On bark of recently killed, dying or diseased trees, often causing small cankers, sometimes on rotting roots.

Thelonectria coronata (Penz. & Sacc.) P. Chaverri & C. Salgado, comb. nov. MycoBank MB518568. Basionym: Nectria coronata Penz. & Sacc., Malpighia 11: 510. 1897.

≡ Neonectria coronata(Penz. & Sacc.) Mantiri & Samuels,Canad. J. Bot. 79: 339. 2001.

Anamorph: “Cylindrocarpon” coronatum Brayford & Samuels, Sydowia 46: 91. 1993.

Habitat: On bark, often associated with small cankers.

Distribution: Probably pantropical.

Descriptions and illustrations: Brayford & Samuels (1993); Samuels & Brayford (1994)

Thelonectria discophora (Mont.) P. Chaverri & C. Salgado, comb. nov. MycoBank MB518569. Basionym: Sphaeria discophora Mont., Ann. Sci. Nat. Bot. II 3: 353. 1835.

≡ Neonectria discophora (Mont.) var. discophora Mantiri & Samuels,
= Nectria tasmanica Berk. in Hooker, Flora Tasmaniae 2: 279. 1860.
= Nectria mammoidea W. Phillips & Plowr. Grevillea 3: 126. 1875.
≡ Creonectria mammoidea (W. Phillips & Plowr.) Seaver, Mycologia 1: 188. 1909 (as Creonectria mammoides).
= Nectria nelumbicola Henn., Verh. Bot. Vereins. Prov. Brandenburg 40: 151. 1898.
= Nectria umbilicata Henn., Hedwigia 41: 3. 1902.
= Nectria mammoidea var. rugulosa Weese, Akad. Wiss. Wien Math.-Naturw. Kl., Abt. 1, 125: 552. 1916.
= Nectria mammoidea var. minor Reinking, Zentralbl. Bakteriol., Abt. 2, 94: 135. 1936.
= Creonectria discostiolata Chardón, Bol. Soc. Venez. Ci. Nat. 5: 341. 1939.
= Nectria pinea Dingley, Trans. Roy. Soc. New Zealand 79: 198. 1951.
Anamorph: “Cylindrocarpon” ianothele var. majus Wollenw., Z. Parasitenk. (Berlin) 1: 161. 1928.
= Cylindrocarpon ianthothele var. minus Reinking, Zentralbl. Bakteriol., Abt. 2, 94: 135. 1936.
= Cylindrocarpon ianthothele var. rugulosum C. Booth, Mycol. Pap. 104: 25. 1966.
= Cylindrocarpon pineum C. Booth, Mycol. Pap. 104: 26. 1966.

Habitat: On bark and twigs of recently killed trees, rarely on palm trunks.

Distribution: Cosmopolitan.

Description and illustrations: Brayford et al. (2004).

Thelonectria jungneri (Henn.) P. Chaverri & C. Salgado, comb. nov. MycoBank MB518570. Basionym: Nectria jungneri Henn., Bot. Jahrb. Syst. 22: 75. 1897.

= Nectria eustoma Penz. & Sacc., Malpighia 11: 509. 1898 [1897]
= Nectria leucoloma Starbäck, Bih. Kongl. Svenska Vetensk.-Akad. Handl. 25: 28. 1899.
= Nectria cinereopapillata Henn. & Nyman in Warburg, Monsunia 1: 161. 1900 [1899]
= Nectria striatospora Zimm., Centralbl. Bakteriol. II, 7: 105. 1901.
= Nectria azureostiolata Doi, Mem. Nat. Sci. Mus. Tokyo 10: 23. 1977.

Anamorph: “Cylindrocarpon” victoriae Wollenw., Z. Parasitenk. (Berlin) 1: 161. 1928.

Habitat: On bark of recently killed or dying trees.

Distribution: Pantropical.

Description and illustrations: Samuels et al. (1990).

Thelonectria lucida (Höhn.) P. Chaverri & C. Salgado, comb. nov. MycoBank MB518571. Basionym: Nectria lucida Höhn., Akad. Wiss. Wien math. Naturw. Kl., Abt. 1, 118: 289. 1909.

≡ Neonectria lucida (Höhn.) Samuels & Brayford, Mycologia 96: 590. 2004.

Anamorph: “Cylindrocarpon” lucidum Booth, Mycol. Pap. 104: 21. 1966.

Habitat: On bark of recently killed or dying trees, rarely on vines.

Distribution: Asia, New Zealand, South America, North America, probably cosmopolitan.

Description and illustrations: Brayford et al. (2004).

Thelonectria olida (Wollenw.) P. Chaverri & C. Salgado, comb. nov. MycoBank MB518572. Basionym: Ramularia olida Wollenw., Phytopathology 3: 223. 1913.

≡ Cylindrocarpon olidum var. olidum (Wollenw.) Wollenw., Fus. Autogr. Del., ed. 1: 471. 1916.
= Cylindrocarpon curvatum Hochapfel in Wollenw., Z. Parasitenk. 3: 495. 1931.

Teleomorph: Unknown.

Habitat: On rotting roots of various plants.

Distribution: Probably widespread.

Descriptions and illustrations: Booth (1966), Brayford (1987).

Notes: This species is somewhat different from the rest of Thelonectria in having shorter macroconidia, fewer septa, and abundant chlamydospores. However, it also has similarities with Thelonectria. Thelonectria olida has short conidiophores, lacks microconidia, and has curved macroconidia with rounded ends. Molecular phylogenetic data presented here also places this species in Thelonectria.

Thelonectria trachosa(Samuels & Brayford) Samuels, P. Chaverri & C. Salgado, comb. nov. MycoBank MB518573. Basionym: Neonectria trachosa Samuels & Brayford, Mycologia 96: 592. 2004.

Anamorph:Cylindrocarpon-like

Habitat: On bark of unknown conifer.

Distribution: Scotland, only known from the type locality.

Description and illustrations: Brayford et al. (2004).

Thelonectria veuillotiana (Sacc. & Roum.) P. Chaverri & C. Salgado, comb. nov. MycoBank MB518574. Basionym: Nectria veuillotiana Sacc. & Roum. in Désmazières, Rev. Mycol. (Toulouse) 2: 189. 1880.

≡ Dialonectria veuillotiana (Sacc. & Roum.) Cooke, Grevillea 12: 110. 1884.
≡ Cucurbitaria veuillotiana (Sacc. & Roum.) Kuntze, Revis. Gen. Pl. (Leipzig) 3: 462. 1898.
≡ Neonectria veuillotiana (Sacc. & Roum.) Mantiri & Samuels, Canad. J. Bot. 79: 339. 2001.
= Sphaerostilbe sanguinea Fuckel, Symb. Myc. App. 3: 22. 1877.

Anamorph: “Cylindrocarpon” candidulum (Sacc.) Wollenw., Z. Parasitenk. 1: 160. 1928.

≡ Atractium candiduli Sacc., Syll. Fung. (Abellini) 2: 512.1883.

Habitat: On bark of recently killed trees, rarely on wood or leaves.

Distribution: Probably widespread.

Description and illustrations: Brayford & Samuels (1993).

Thelonectria viridispora (Samuels & Brayford) P. Chaverri, C. Salgado, & Samuels, comb. nov. MycoBank MB518575. Basionym: Neonectria viridispora Samuels & Brayford, Mycologia 96: 592. 2004.

Anamorph: Cylindrocarpon-like.

Habitat: On bark of Ochroma.

Distribution: Ecuador, only known from the type locality.

Description and illustrations: Brayford et al. (2004).

Thelonectria westlandica (Dingley) P. Chaverri & C. Salgado, comb. nov. MycoBank MB518576. Basionym: Nectria westlandica Dingley, Trans. Roy. Soc. New Zealand 79: 201. 1951.

≡Neonectria westlandica (Dingley) Samuels & Brayford, Mycologia 96: 595. 2004.

Anamorph: Cylindrocarpon-like.

Habitat: On bark of dicotyledonous trees, sometimes gymnosperms.

Distribution: New Zealand.

Description and illustrations: Brayford et al. (2004)

Acknowledgments

We appreciate the nomenclatural advice given by Drew Minnis (USDA). We also greatly appreciate the assistance of Adam Bazinet (UMD) in the use of the GRID-computing system. This study was funded by a grant from United States National Science Foundation (PEET program) DEB-0925696: “Monographic Studies in the Nectriaceae, Hypocreales: Nectria, Cosmospora, and Neonectria” to University of Maryland (P. Chaverri, G.J. Samuels & A.Y. Rossman).

Taxonomic novelties: Ilyonectria P. Chaverri & C. Salgado, gen. nov.; Ilyonectria coprosmae (Dingley) P. Chaverri & C. Salgado, comb. nov.; Ilyonectria liriodendri (Halleen et al.) P. Chaverri & C. Salgado, comb. nov.; Ilyonectria macrodydima (Halleen, Schroers & Crous) P. Chaverri & C. Salgado, comb. nov.; Ilyonectria radicicola (Gerlach & L. Nilsoon) P. Chaverri & C. Salgado, comb. nov.; Rugonectria P. Chaverri & Samuels, gen. nov.; Rugonectria castaneicola (W. Yamam. & Oyasu) Hirooka & P. Chaverri, comb. nov; Rugonectria neobalansae (Samuels) P. Chaverri & Samuels, comb. nov.; Rugonectria rugulosa (Pat. & Gaill.) Samuels, P. Chaverri & C. Salgado, comb. nov.; Thelonectria P. Chaverri & C. Salgado, gen. nov.; Thelonectria coronata (Penz. & Sacc.) P. Chaverri & C. Salgado, comb. nov.; Thelonectria discophora (Mont.) P. Chaverri & C. Salgado, comb. nov.; Thelonectria jungneri (Henn.) P. Chaverri & C. Salgado, comb. nov.; Thelonectria lucida (Höhnel) P. Chaverri & C. Salgado, comb. nov.; Thelonectria olida (Wollenw.) P. Chaverri & C. Salgado, comb. nov; Thelonectria trachosa (Samuels & Brayford) Samuels, P. Chaverri & C. Salgado, comb. nov.; Thelonectria veuillotiana (Sacc. & Roum.) P. Chaverri & C. Salgado, comb. nov.; Thelonectria viridispora (Samuels & Brayford) P. Chaverri, C. Salgado, & Samuels, comb. nov.; Thelonectria westlandica (Dingley) P. Chaverri & C. Salgado, comb. nov.

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PERMALINK

Delimitation of Neonectria and Cylindrocarpon (Nectriaceae, Hypocreales, Ascomycota) and related genera with Cylindrocarpon-like anamorphs

P Chaverri

C Salgado

Y Hirooka

AY Rossman

GJ Samuels

Abstract

INTRODUCTION

MATERIALS AND METHODS

Morphological characterisation

DNA extraction, polymerase chain reaction (PCR), and sequencing

Table 1.

Table 2.

Phylogenetic analyses

RESULTS

Molecular phylogenetic analyses

Fig. 1.

Table 3.

Morphological characterisation

Presence or absence of a stroma

Perithecial wall

Ascospores

Paraphyses

Conidiophores and phialides

Macro- and microconidia

Chlamydospores

Ecology

DISCUSSION

Genus concept

Neonectria/Cylindrocarpon sensu stricto

The segregate genera: Campylocarpon, Ilyonectria, Rugonectria, and Thelonectria

Species of Neonectria/Cylindrocarpon of uncertain classification

TAXONOMY

KEY TO SEGREGATE GENERA OF NEONECTRIA/CYLINDROCARPON

DESCRIPTION OF GENERA

Fig. 2.

Fig. 3.

Fig. 4.

Fig. 5.

Fig. 6.

Acknowledgments

References

ACTIONS

PERMALINK

RESOURCES

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