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Relative spot intensity versus time following 408 nm laser damage. Plots show means and standard error of the means of 21–25 samples treated over three independent experiments for each variant. Comparisons were made using two-sided t-test: 499 wild-type (499 wt) and 499 mutant (c.1496C>T), P = 0.357, variant 302 (c.905T>C) and 499 wt, P < 0.001, variant 393 (c.1177C>T) and 499 wt, P = 0.004, variant 690 (c.2059G>C) and 499 wt, P < 0.001.
