Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1995 Jan 11;23(1):192–193. doi: 10.1093/nar/23.1.192

Low stringency-PCR (LS-PCR) allows entirely internally standardized DNA quantitation.

O L Caballero 1, L L Villa 1, A J Simpson 1
PMCID: PMC306649  PMID: 7870586

Full text

PDF
192

Images in this article

193Image
on p.193

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Becker-André M., Hahlbrock K. Absolute mRNA quantification using the polymerase chain reaction (PCR). A novel approach by a PCR aided transcript titration assay (PATTY). Nucleic Acids Res. 1989 Nov 25;17(22):9437–9446. doi: 10.1093/nar/17.22.9437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berumen J., Casas L., Segura E., Amezcua J. L., Garcia-Carranca A. Genome amplification of human papillomavirus types 16 and 18 in cervical carcinomas is related to the retention of E1/E2 genes. Int J Cancer. 1994 Mar 1;56(5):640–645. doi: 10.1002/ijc.2910560506. [DOI] [PubMed] [Google Scholar]
  3. Dias Neto E., Santos F. R., Pena S. D., Simpson A. J. Sex determination by low stringency PCR (LS-PCR). Nucleic Acids Res. 1993 Feb 11;21(3):763–764. doi: 10.1093/nar/21.3.763. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dias Neto E., Santos F. R., Pena S. D., Simpson A. J. Sex determination by low stringency PCR (LS-PCR). Nucleic Acids Res. 1993 Feb 11;21(3):763–764. doi: 10.1093/nar/21.3.763. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gilliland G., Perrin S., Blanchard K., Bunn H. F. Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction. Proc Natl Acad Sci U S A. 1990 Apr;87(7):2725–2729. doi: 10.1073/pnas.87.7.2725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Sanguinetti C. J., Dias Neto E., Simpson A. J. Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. Biotechniques. 1994 Nov;17(5):914–921. [PubMed] [Google Scholar]
  7. Siebert P. D., Larrick J. W. Competitive PCR. Nature. 1992 Oct 8;359(6395):557–558. doi: 10.1038/359557a0. [DOI] [PubMed] [Google Scholar]
  8. Terry G., Ho L., Jenkins D., Hills M., Singer A., Mansell B., Beverley E. Definition of human papillomavirus type 16 DNA levels in low and high grade cervical lesions by a simple polymerase chain reaction technique. Arch Virol. 1993;128(1-2):123–133. doi: 10.1007/BF01309793. [DOI] [PubMed] [Google Scholar]
  9. Yee C., Krishnan-Hewlett I., Baker C. C., Schlegel R., Howley P. M. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am J Pathol. 1985 Jun;119(3):361–366. [PMC free article] [PubMed] [Google Scholar]
  10. Zachar V., Thomas R. A., Goustin A. S. Absolute quantification of target DNA: a simple competitive PCR for efficient analysis of multiple samples. Nucleic Acids Res. 1993 Apr 25;21(8):2017–2018. doi: 10.1093/nar/21.8.2017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. de Caballero O. L., Dias Neto E., Koury M. C., Romanha A. J., Simpson A. J. Low-stringency PCR provides an internal control for negative results in PCR-based diagnosis. PCR Methods Appl. 1994 Apr;3(5):305–307. doi: 10.1101/gr.3.5.305. [DOI] [PubMed] [Google Scholar]
  12. de Caballero O. L., Dias Neto E., Koury M. C., Romanha A. J., Simpson A. J. Low-stringency PCR with diagnostically useful primers for identification of Leptospira serovars. J Clin Microbiol. 1994 May;32(5):1369–1372. doi: 10.1128/jcm.32.5.1369-1372.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. zur Hausen H. Human papillomaviruses in the pathogenesis of anogenital cancer. Virology. 1991 Sep;184(1):9–13. doi: 10.1016/0042-6822(91)90816-t. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES