Membrane Topology and Identification of Critical Amino Acid Residues in the Wzx O-Antigen Translocase from Escherichia coli O157:H7

Volume 192, no. 23, p. 6160-6171, 2010. Page 6160: The title should appear as shown above.

Page 6165: Fig. 3 should appear as shown below. (The wzx_EcO157 gene was obtained by PCR cloning from genomic DNA of strain UWO934-88, a clinical isolate of E. coli O157:H7 [Marolda et al., Microbiology 150:4095-4105, 2004], and its amino acid sequence is identical to that of GenBank accession no. AE005429_8. The amino acid sequence of Wzx_EcO157 includes an N-terminal fusion to the FLAG epitope. To construct this fusion, the first Met of wild-type Wzx_EcO157 was changed to Gly [position 1/13]. The amino acid numbers in the figure correspond to the original positions in the wild-type protein [Gly-13 corresponds to Met-1]. The tyrosine residue at position 319 in the original figure was misplaced, and its normal location is at position 253. The methionine at position 405 was displaced graphically but is actually located at the end of cytosolic loop 5.)

FIG. 3.

Page 6168: Table 3 should appear as shown below (column heads have been corrected).

TABLE 3.

Plasmid	Biotinylationa		Location of amino acid residueb	O-antigen expressionc
	Whole cells	Vesicles
pML203-K144C	+	ND	P	100
pML203-K176C	ND	+	C	40
pML203-H209C	+	ND	P	90
pML203-K244C	−	+	C	60
pML203-P254C	ND	−	TM	100
pML203-F264C	ND	−	TM	100
pML203-D277C	+	+	P	100
pML203-G286C	ND	−	?	100
pML203-F293C	−	−	?	100
pML203-R298C	+	ND	P	0
pML203-P306C	−	−	TM	70
pML203-P313C	−	−	TM	50
pML203-D320C	−	+	C	30
pML203-R324C	−	+	C	90
pML203-K331C	−	+	C	70
pML203-K367C	+	+	P	90
pML203-K402C	ND	ND	C	80