Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1995 Mar 11;23(5):856–860. doi: 10.1093/nar/23.5.856

Successful transformation of yeast mitochondria with RPM1: an approach for in vivo studies of mitochondrial RNase P RNA structure, function and biosynthesis.

P Sulo 1, K R Groom 1, C Wise 1, M Steffen 1, N Martin 1
PMCID: PMC306770  PMID: 7708503

Abstract

Mitochondrial RNase P RNA (Rpm1r) is coded by the RPM1 gene of mitochondrial DNA in many yeasts. As an initial step to developing a genetic approach to the structure and biogenesis of yeast mitochondrial RNase P, biolistic transformation has been used to introduce wild type and altered RPM1 genes into strains containing no mitochondrial DNA. The introduced wild type gene does support RNase P activity demonstrating that pre-existing RNase P activity is not necessary for the biosynthesis of the enzyme. Mutations introduced into RPM1 in vitro result in reduced accumulation of mature tRNA and in an alteration of the processing of Rpm1r in vivo.

Full text

PDF
856

Images in this article

859Image
on p.859
859Image
on p.859
859Image
on p.859

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anziano P. Q., Butow R. A. Splicing-defective mutants of the yeast mitochondrial COXI gene can be corrected by transformation with a hybrid maturase gene. Proc Natl Acad Sci U S A. 1991 Jul 1;88(13):5592–5596. doi: 10.1073/pnas.88.13.5592. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Attardi G., Schatz G. Biogenesis of mitochondria. Annu Rev Cell Biol. 1988;4:289–333. doi: 10.1146/annurev.cb.04.110188.001445. [DOI] [PubMed] [Google Scholar]
  3. Biswas T. K. In vitro transcription analysis of the region of Saccharomyces cerevisiae mitochondrial DNA containing the tRNA(fMet) gene. Nucleic Acids Res. 1991 Nov 11;19(21):5937–5942. doi: 10.1093/nar/19.21.5937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brown J. W., Pace N. R. Ribonuclease P RNA and protein subunits from bacteria. Nucleic Acids Res. 1992 Apr 11;20(7):1451–1456. doi: 10.1093/nar/20.7.1451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dang Y. L., Martin N. C. Yeast mitochondrial RNase P. Sequence of the RPM2 gene and demonstration that its product is a protein subunit of the enzyme. J Biol Chem. 1993 Sep 15;268(26):19791–19796. [PubMed] [Google Scholar]
  6. Fangman W. L., Henly J. W., Brewer B. J. RPO41-independent maintenance of [rho-] mitochondrial DNA in Saccharomyces cerevisiae. Mol Cell Biol. 1990 Jan;10(1):10–15. doi: 10.1128/mcb.10.1.10. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fox T. D., Sanford J. C., McMullin T. W. Plasmids can stably transform yeast mitochondria lacking endogenous mtDNA. Proc Natl Acad Sci U S A. 1988 Oct;85(19):7288–7292. doi: 10.1073/pnas.85.19.7288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Francisci S., Palleschi C., Ragnini A., Frontali L. Analysis of transcripts of the major cluster of tRNA genes in the mitochondrial genome of S. cerevisiae. Nucleic Acids Res. 1987 Aug 25;15(16):6387–6403. doi: 10.1093/nar/15.16.6387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Haas E. S., Morse D. P., Brown J. W., Schmidt F. J., Pace N. R. Long-range structure in ribonuclease P RNA. Science. 1991 Nov 8;254(5033):853–856. doi: 10.1126/science.1719634. [DOI] [PubMed] [Google Scholar]
  10. Hinnen A., Hicks J. B., Fink G. R. Transformation of yeast. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929–1933. doi: 10.1073/pnas.75.4.1929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hollingsworth M. J., Martin N. C. RNase P activity in the mitochondria of Saccharomyces cerevisiae depends on both mitochondrion and nucleus-encoded components. Mol Cell Biol. 1986 Apr;6(4):1058–1064. doi: 10.1128/mcb.6.4.1058. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ito H., Fukuda Y., Murata K., Kimura A. Transformation of intact yeast cells treated with alkali cations. J Bacteriol. 1983 Jan;153(1):163–168. doi: 10.1128/jb.153.1.163-168.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Johnston S. A., Anziano P. Q., Shark K., Sanford J. C., Butow R. A. Mitochondrial transformation in yeast by bombardment with microprojectiles. Science. 1988 Jun 10;240(4858):1538–1541. doi: 10.1126/science.2836954. [DOI] [PubMed] [Google Scholar]
  14. Martin N. C., Underbrink-Lyon K. A mitochondrial locus is necessary for the synthesis of mitochondrial tRNA in the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1981 Aug;78(8):4743–4747. doi: 10.1073/pnas.78.8.4743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Miller D. L., Martin N. C. Characterization of the yeast mitochondrial locus necessary for tRNA biosynthesis: DNA sequence analysis and identification of a new transcript. Cell. 1983 Oct;34(3):911–917. doi: 10.1016/0092-8674(83)90548-2. [DOI] [PubMed] [Google Scholar]
  16. Morales M. J., Dang Y. L., Lou Y. C., Sulo P., Martin N. C. A 105-kDa protein is required for yeast mitochondrial RNase P activity. Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9875–9879. doi: 10.1073/pnas.89.20.9875. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Myers A. M., Pape L. K., Tzagoloff A. Mitochondrial protein synthesis is required for maintenance of intact mitochondrial genomes in Saccharomyces cerevisiae. EMBO J. 1985 Aug;4(8):2087–2092. doi: 10.1002/j.1460-2075.1985.tb03896.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Najarian D., Shu H. H., Martin N. C. Sequence and expression of four mutant aspartic acid tRNA genes from the mitochondria of Saccharomyces cerevisiae. Nucleic Acids Res. 1986 Dec 22;14(24):9561–9578. doi: 10.1093/nar/14.24.9561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ragnini A., Grisanti P., Rinaldi T., Frontali L., Palleschi C. Mitochondrial genome of Saccharomyces douglasii: genes coding for components of the protein synthetic apparatus. Curr Genet. 1991 Mar;19(3):169–174. doi: 10.1007/BF00336483. [DOI] [PubMed] [Google Scholar]
  20. Rothstein R. Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast. Methods Enzymol. 1991;194:281–301. doi: 10.1016/0076-6879(91)94022-5. [DOI] [PubMed] [Google Scholar]
  21. Shu H. H., Wise C. A., Clark-Walker G. D., Martin N. C. A gene required for RNase P activity in Candida (Torulopsis) glabrata mitochondria codes for a 227-nucleotide RNA with homology to bacterial RNase P RNA. Mol Cell Biol. 1991 Mar;11(3):1662–1667. doi: 10.1128/mcb.11.3.1662. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Underbrink-Lyon K., Miller D. L., Ross N. A., Fukuhara H., Martin N. C. Characterization of a yeast mitochondrial locus necessary for tRNA biosynthesis. Deletion mapping and restriction mapping studies. Mol Gen Genet. 1983;191(3):512–518. doi: 10.1007/BF00425771. [DOI] [PubMed] [Google Scholar]
  23. Valens M., Rinaldi T., Daignan-Fornier B., Bolotin-Fukuhara M. Identification of nuclear genes which participate to mitochondrial translation in Saccharomyces cerevisiae. Biochimie. 1991 Dec;73(12):1525–1532. doi: 10.1016/0300-9084(91)90187-6. [DOI] [PubMed] [Google Scholar]
  24. Wise C. A., Martin N. C. Dramatic size variation of yeast mitochondrial RNAs suggests that RNase P RNAs can be quite small. J Biol Chem. 1991 Oct 15;266(29):19154–19157. [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES