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. 1995 Mar 25;23(6):995–1002. doi: 10.1093/nar/23.6.995

Protein binding interactions at the STB locus of the yeast 2 microns plasmid.

C Hadfield 1, R C Mount 1, A M Cashmore 1
PMCID: PMC306797  PMID: 7731815

Abstract

The cis-acting STB locus has been shown to be a multiple protein binding site. STB-specific binding activity was detected in a normally insoluble yeast cell protein fraction, suggesting association with a subcellular structure. Both 2 microns-encoded and host-encoded STB-binding activities were identified. The 2 microns proteins showed contrasting STB-binding activities: C (REP2) protein acted cooperatively with the host factor to promote STB binding; B (REP1) protein also acted in association with the host factor, but showed a dual action, opposing or facilitating binding, depending upon concentration; D (RAF) exhibited rapid binding and antagonism to host factor binding. FLP did not bind, but promoted host factor dissociation. The implications of these activities for the molecular mechanism of 2 microns plasmid inheritance are considered.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beggs J. D., Guerineau M., Atkins J. F. A map of the restriction targets in yeast 2 micron plasmid DNA cloned on bacteriophage lambda. Mol Gen Genet. 1976 Nov 17;148(3):287–294. doi: 10.1007/BF00332903. [DOI] [PubMed] [Google Scholar]
  2. Berman J., Eisenberg S., Tye B. K. An agarose gel electrophoresis assay for the detection of DNA-binding activities in yeast cell extracts. Methods Enzymol. 1987;155:528–537. doi: 10.1016/0076-6879(87)55034-0. [DOI] [PubMed] [Google Scholar]
  3. Bondeson K., Frostell-Karlsson A., Fägerstam L., Magnusson G. Lactose repressor-operator DNA interactions: kinetic analysis by a surface plasmon resonance biosensor. Anal Biochem. 1993 Oct;214(1):245–251. doi: 10.1006/abio.1993.1484. [DOI] [PubMed] [Google Scholar]
  4. Broach J. R., Hicks J. B. Replication and recombination functions associated with the yeast plasmid, 2 mu circle. Cell. 1980 Sep;21(2):501–508. doi: 10.1016/0092-8674(80)90487-0. [DOI] [PubMed] [Google Scholar]
  5. Buchman A. R., Kimmerly W. J., Rine J., Kornberg R. D. Two DNA-binding factors recognize specific sequences at silencers, upstream activating sequences, autonomously replicating sequences, and telomeres in Saccharomyces cerevisiae. Mol Cell Biol. 1988 Jan;8(1):210–225. doi: 10.1128/mcb.8.1.210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Futcher A. B., Cox B. S. Maintenance of the 2 microns circle plasmid in populations of Saccharomyces cerevisiae. J Bacteriol. 1983 May;154(2):612–622. doi: 10.1128/jb.154.2.612-622.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Guerineau M., Grandchamp C., Slonimski P. P. Circular DNA of a yeast episome with two inverted repeats: structural analysis by a restriction enzyme and electron microscopy. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3030–3034. doi: 10.1073/pnas.73.9.3030. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hartley J. L., Donelson J. E. Nucleotide sequence of the yeast plasmid. Nature. 1980 Aug 28;286(5776):860–865. doi: 10.1038/286860a0. [DOI] [PubMed] [Google Scholar]
  9. Jayaram M., Sutton A., Broach J. R. Properties of REP3: a cis-acting locus required for stable propagation of the Saccharomyces cerevisiae plasmid 2 microns circle. Mol Cell Biol. 1985 Sep;5(9):2466–2475. doi: 10.1128/mcb.5.9.2466. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jönsson U., Fägerstam L., Ivarsson B., Johnsson B., Karlsson R., Lundh K., Löfås S., Persson B., Roos H., Rönnberg I. Real-time biospecific interaction analysis using surface plasmon resonance and a sensor chip technology. Biotechniques. 1991 Nov;11(5):620–627. [PubMed] [Google Scholar]
  11. Kikuchi Y. Yeast plasmid requires a cis-acting locus and two plasmid proteins for its stable maintenance. Cell. 1983 Dec;35(2 Pt 1):487–493. doi: 10.1016/0092-8674(83)90182-4. [DOI] [PubMed] [Google Scholar]
  12. Livingston D. M. A sequence of the yeast 2 micron DNA plasmid chromosome near the origin of replication is exposed to restriction endonuclease digestion. J Mol Biol. 1982 Sep 25;160(3):397–410. doi: 10.1016/0022-2836(82)90304-7. [DOI] [PubMed] [Google Scholar]
  13. Murray J. A., Cesareni G. Functional analysis of the yeast plasmid partition locus STB. EMBO J. 1986 Dec 1;5(12):3391–3399. doi: 10.1002/j.1460-2075.1986.tb04655.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Murray J. A., Scarpa M., Rossi N., Cesareni G. Antagonistic controls regulate copy number of the yeast 2 mu plasmid. EMBO J. 1987 Dec 20;6(13):4205–4212. doi: 10.1002/j.1460-2075.1987.tb02768.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Som T., Armstrong K. A., Volkert F. C., Broach J. R. Autoregulation of 2 micron circle gene expression provides a model for maintenance of stable plasmid copy levels. Cell. 1988 Jan 15;52(1):27–37. doi: 10.1016/0092-8674(88)90528-4. [DOI] [PubMed] [Google Scholar]
  16. Taketo M., Jazwinski S. M., Edelman G. M. Association of the 2-micron DNA plasmid with yeast folded chromosomes. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3144–3148. doi: 10.1073/pnas.77.6.3144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Veit B. E., Fangman W. L. Chromatin organization of the Saccharomyces cerevisiae 2 microns plasmid depends on plasmid-encoded products. Mol Cell Biol. 1985 Sep;5(9):2190–2196. doi: 10.1128/mcb.5.9.2190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Volkert F. C., Broach J. R. Site-specific recombination promotes plasmid amplification in yeast. Cell. 1986 Aug 15;46(4):541–550. doi: 10.1016/0092-8674(86)90879-2. [DOI] [PubMed] [Google Scholar]
  19. Wu L. C., Fisher P. A., Broach J. R. A yeast plasmid partitioning protein is a karyoskeletal component. J Biol Chem. 1987 Jan 15;262(2):883–891. [PubMed] [Google Scholar]

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